FastQCFastQC Report
Thu 26 May 2016
SRR1546437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546437_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2319612
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT247781.0681958879329818No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC232221.0011157038332272No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199730.861049175465552No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC148860.6417452573964956No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC145570.6275618508612647No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG125430.5407369853234075No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT120470.5193540988751567No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87070.37536450061475796No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG84320.3635090696202641No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84280.36333662698761693No Hit
GAATATGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT84150.36277618843151355No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG73670.3175962186779513No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61400.26469944111342764No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC42900.18494472351410493No Hit
GAACTGACTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC41020.1768399197796873No Hit
GCTGTCACTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG35850.15455170951003877TruSeq Adapter, Index 13 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT35650.15368949634680282No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC27890.12023562561324912No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC27130.11695921559295261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGT207.858141E-444.00000440
ATATACG408.3182385E-944.0000041
ATCGCCT207.858141E-444.00000436
CGAAGTA207.858141E-444.0000044
GTCGATC351.4469333E-744.041
GTTCGAC254.4442462E-544.016
TACGAGT254.4442462E-544.03
TTGCGAC502.7284841E-1144.030
TATACGC254.4442462E-544.022
TCGGTAT302.5288355E-644.030
CGTTTTA132800.043.834341
CGTTATT73200.043.6393431
ATAATAC55350.043.4037933
GTTATTT75450.042.8045042
TCGATAG1750.042.742862
GTTTTAT140100.042.7280542
AAACGGC27150.042.70356
TAGGCCG1400.042.4285746
TAATACG41150.042.2357254
TGAAACG27050.042.1293874
Produced by FastQC (version 0.11.5)