SRR1546436_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546436_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1023118
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	15347	1.500022480300415	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	8829	0.8629503146264653	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	8673	0.8477028065188961	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	7299	0.7134074466483827	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	7132	0.6970847937383567	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5211	0.5093254150547639	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4917	0.4805897266981912	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	3975	0.38851823543325403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	2760	0.26976360498007074	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2531	0.24738104500165184	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	2022	0.1976311627788779	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1890	0.18472942514939625	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1835	0.17935370113711224	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1760	0.17202316839308857	No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1676	0.16381297171978207	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGCA	1549	0.15139993627323536	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1548	0.15130219583664836	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1463	0.1429942587267549	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGCTGACCCAGCCACTTT	1281	0.12520549926792413	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	1274	0.12452131621181525	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGACTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1258	0.12295746922642355	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGGCAGCCACTTTCGTATGC	1223	0.11953655394587917	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCCTC	1065	0.10409356496513598	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	7.032924E-4	45.0	22
ATCCGGC	40	6.8139343E-9	45.0	28
ATCCGAT	20	7.032924E-4	45.0	26
ACGTCAA	20	7.032924E-4	45.0	10
CGCGTTC	20	7.032924E-4	45.0	24
CGGTATA	50	2.1827873E-11	45.0	31
CACGTAA	20	7.032924E-4	45.0	1
TGCGTTA	35	1.2117562E-7	45.0	1
TACCGTT	20	7.032924E-4	45.0	13
AATCGCG	25	3.8905047E-5	45.0	21
GTACGAG	45	3.8380676E-10	45.0	2
CTTACGT	25	3.8905047E-5	45.0	41
TACCGCT	45	3.8380676E-10	45.0	29
ACGGTAC	40	6.8139343E-9	45.0	30
CATTGAC	65	0.0	44.999996	26
TGCGCAA	30	2.1652668E-6	44.999996	1
CGTTTTA	3280	0.0	44.79421	1
CGTTATT	780	0.0	44.711536	1
GATGAAT	11170	0.0	44.536705	20
CCGATGA	10890	0.0	44.380165	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content