##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546436_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1023118
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.778059813237572	31.0	30.0	31.0	30.0	33.0
2	30.87273510973319	31.0	30.0	31.0	28.0	34.0
3	30.80461784466699	31.0	30.0	33.0	28.0	34.0
4	34.80224372946229	35.0	35.0	37.0	32.0	37.0
5	32.731096510861896	35.0	35.0	37.0	28.0	37.0
6	33.83131075789889	35.0	35.0	37.0	29.0	37.0
7	18.734433369366975	30.0	0.0	35.0	0.0	37.0
8	26.249857787664766	30.0	17.0	35.0	17.0	37.0
9	32.70483951997717	32.0	32.0	37.0	27.0	39.0
10	35.03575247429915	37.0	34.0	37.0	30.0	39.0
11	35.949322561034016	37.0	35.0	39.0	32.0	39.0
12	35.65886144120228	37.0	35.0	39.0	30.0	39.0
13	35.175627835694414	37.0	34.0	39.0	29.0	39.0
14	36.407579575376445	38.0	35.0	40.0	30.0	41.0
15	36.72030107964086	38.0	35.0	40.0	31.0	41.0
16	37.10263332284253	38.0	35.0	40.0	32.0	41.0
17	36.12651131150073	38.0	34.0	40.0	29.0	41.0
18	36.450293123569324	38.0	35.0	40.0	30.0	41.0
19	36.49056218344316	38.0	35.0	40.0	30.0	41.0
20	35.692835039555554	38.0	34.0	40.0	27.0	41.0
21	35.94270651088144	38.0	34.0	40.0	30.0	41.0
22	35.55500734030679	38.0	34.0	40.0	28.0	41.0
23	35.485387804730244	37.0	34.0	40.0	27.0	41.0
24	35.66922681450234	37.0	34.0	40.0	29.0	41.0
25	35.98795544599938	37.0	34.0	40.0	30.0	41.0
26	35.471565352188115	37.0	34.0	40.0	29.0	41.0
27	34.749820646298865	37.0	33.0	40.0	25.0	41.0
28	34.609070507996144	36.0	33.0	40.0	24.0	41.0
29	34.54759959261786	36.0	33.0	40.0	24.0	41.0
30	34.30469603701626	36.0	33.0	40.0	23.0	41.0
31	34.646352620127885	37.0	33.0	40.0	24.0	41.0
32	33.42367938009105	36.0	32.0	40.0	18.0	41.0
33	32.863918922353044	36.0	31.0	40.0	15.0	41.0
34	32.13192026726145	36.0	31.0	40.0	10.0	41.0
35	31.431552372258135	36.0	29.0	40.0	7.0	41.0
36	31.455116614114893	36.0	28.0	40.0	9.0	41.0
37	31.309369007289483	36.0	28.0	40.0	8.0	41.0
38	31.491838673544986	35.0	28.0	40.0	10.0	41.0
39	31.29032623802924	35.0	27.0	40.0	10.0	41.0
40	30.958418286062802	35.0	26.0	40.0	8.0	41.0
41	31.222464075502533	35.0	27.0	40.0	10.0	41.0
42	30.712197420043434	35.0	26.0	40.0	8.0	41.0
43	30.372158441157325	35.0	24.0	40.0	7.0	41.0
44	30.556585848357667	35.0	25.0	40.0	8.0	41.0
45	30.675116653211067	35.0	26.0	39.0	9.0	40.0
46	30.44637079984909	35.0	25.0	39.0	8.0	40.0
47	30.433254033259114	35.0	26.0	39.0	8.0	40.0
48	30.60929433359593	35.0	26.0	39.0	8.0	40.0
49	30.473426330100732	35.0	26.0	39.0	8.0	40.0
50	30.098089369945598	35.0	24.0	39.0	7.0	40.0
51	30.113874450454396	35.0	24.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	6.0
14	16.0
15	52.0
16	177.0
17	487.0
18	1179.0
19	2665.0
20	5611.0
21	10757.0
22	18291.0
23	26992.0
24	34873.0
25	40202.0
26	41840.0
27	38757.0
28	33640.0
29	31535.0
30	33125.0
31	39741.0
32	50211.0
33	70829.0
34	84907.0
35	86696.0
36	92885.0
37	131803.0
38	120935.0
39	24900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.610201364847455	17.380888616953275	21.472303292484344	16.536606725714922
2	27.611771076259046	40.189205937145076	21.990132125522177	10.208890861073698
3	21.348270678455467	38.7647368143264	30.153706610576688	9.733285896641442
4	19.251640573228112	19.64221135782969	49.804910088572385	11.30123798036981
5	34.33240349597993	28.043783805973504	25.609655973211304	12.014156724835258
6	15.941269726463613	48.254062581246735	27.6819487097285	8.122718982561151
7	28.043588325100334	46.8427884173673	24.19505863448791	0.9185646230444582
8	61.95248250934887	21.87020460982995	13.605859734654263	2.5714531461669132
9	59.0526214962497	4.542584530816582	13.411063044536405	22.99373092839731
10	52.77035493462142	18.164669177944283	18.76323161160296	10.301744275831332
11	48.896803692242734	14.510349734830196	24.627462325948716	11.965384246978354
12	32.61891590217355	15.541120379076508	26.22952582204594	25.610437896703996
13	29.353310175365895	29.736648167660036	29.046502944919357	11.86353871205472
14	11.918175616106842	46.58778361831187	26.082230984109362	15.411809781471932
15	9.722827669926636	18.919909531451896	46.661186686188685	24.69607611243278
16	10.74255364483862	16.215529391526687	40.26778924816101	32.774127715473675
17	11.39839197433727	29.26456185894491	31.624211479027835	27.712834687689984
18	14.358167875064265	20.862598449054754	34.07788739910743	30.70134627677355
19	20.536047650417643	21.408283306519873	26.17058833878399	31.88508070427849
20	34.91816193244572	19.397273823742715	30.309602606932923	15.37496163687864
21	16.433490565115658	38.36927900789547	27.90557882863951	17.291651598349357
22	16.452647690686707	19.73682410044589	37.9418600787006	25.868668130166807
23	27.20546408136696	27.33858655599843	27.618710647256716	17.837238715377893
24	16.432122199003437	30.75959957697939	35.12146204054664	17.686816183470526
25	13.312638424893317	38.24006615072748	32.179377158841895	16.26791826553731
26	12.910729749647645	25.777574043267737	42.095437671900996	19.216258535183623
27	27.71430079423879	26.988578052580447	28.02149898643167	17.275622166749095
28	10.72896772415303	35.54018207088527	37.4230538412969	16.3077963636648
29	15.264710424408523	21.12170834644684	44.74185773293012	18.871723496214514
30	15.523038398307918	38.90176890642135	29.94014375663413	15.6350489386366
31	21.80843265390698	20.999044098530177	24.318700286770444	32.8738229607924
32	35.806720241457974	22.63785799878411	25.546711132049282	16.00871062770863
33	32.010286203546414	22.909380931622746	23.395053161023462	21.68527970380738
34	16.70804345148849	23.992149488133332	27.315031110780964	31.98477594959721
35	25.905125313013748	20.72869405093059	31.90932033255206	21.456860303503603
36	21.337323749557726	31.614437435369137	26.068449582550596	20.979789232522545
37	18.615448071483446	26.56282071080755	32.05436714044714	22.76736407726186
38	15.972937627917796	30.568419283015253	24.044636102580544	29.414006986486406
39	17.65983982297252	28.709689400440613	26.073923046999465	27.5565477295874
40	18.44977803146851	24.821379352137292	28.680562750337696	28.048279866056507
41	13.602047857627369	22.168997124476356	27.953373902130547	36.275581115765725
42	17.304162374232494	23.372866081918215	30.721285325837293	28.601686218012002
43	19.68404426468892	26.143025535666464	26.740806045832443	27.432124153812172
44	19.45640678787784	25.32053976178701	29.634998113609573	25.588055336725578
45	15.881257098399207	29.346468344804805	26.633878008206285	28.138396548589707
46	24.73136041004068	32.11995097339701	23.330739953749223	19.817948662813087
47	17.1096589054244	29.355069503224456	32.62321648138338	20.912055109967763
48	20.69614648554712	23.744377481385335	28.441489642445934	27.117986390621613
49	20.249179469034853	21.711376400376107	34.96967114252706	23.06977298806198
50	18.02558453668101	28.23916693871088	29.762353902482413	23.972894622125697
51	17.940843578160095	29.07680248026132	26.293741288883588	26.688612652695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1246.0
1	1491.5
2	1737.0
3	10183.5
4	18630.0
5	13882.5
6	9135.0
7	9262.5
8	9390.0
9	9529.0
10	9668.0
11	9635.0
12	9602.0
13	9187.5
14	8773.0
15	8255.0
16	7737.0
17	7222.0
18	6707.0
19	6193.0
20	5679.0
21	5535.0
22	5391.0
23	5139.0
24	4887.0
25	5717.0
26	7740.5
27	8934.0
28	10642.5
29	12351.0
30	13893.0
31	15435.0
32	18131.5
33	20828.0
34	22078.0
35	23328.0
36	25431.0
37	27534.0
38	34582.0
39	41630.0
40	52343.0
41	63056.0
42	69149.5
43	75243.0
44	81728.5
45	88214.0
46	109765.0
47	131316.0
48	102741.5
49	74167.0
50	70678.5
51	67190.0
52	65331.5
53	63473.0
54	60766.0
55	58059.0
56	52701.0
57	47343.0
58	41839.5
59	36336.0
60	30530.0
61	24724.0
62	20069.5
63	15415.0
64	12034.0
65	8653.0
66	6795.5
67	4938.0
68	4164.0
69	3390.0
70	3052.5
71	2715.0
72	2227.0
73	1739.0
74	1280.0
75	666.5
76	512.0
77	404.5
78	297.0
79	219.5
80	142.0
81	106.5
82	71.0
83	67.0
84	63.0
85	48.5
86	34.0
87	28.5
88	23.0
89	17.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1023118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.276653695908756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.61247198270665	22.511743001302342
2	5.991581104965453	3.3884372799211686
3	2.371559150949534	2.011792704922901
4	1.4850295621581138	1.6796666662932793
5	1.0084208641557937	1.4257383777731214
6	0.7920944398621411	1.343866810226195
7	0.6635568695885683	1.3134217466228062
8	0.5722798871367326	1.2945728148559308
9	0.488969170813665	1.2443770719966292
>10	6.407170616928711	40.82270207074175
>50	0.5134478623296098	9.150212036120372
>100	0.08032574571188865	4.08161390588376
>500	0.004600152838125731	0.9738759596481267
>1k	0.006369442391251012	3.5057733482165223
>5k	0.001769289553125281	3.716598031970617
>10k+	3.538579106250562E-4	1.5356081735045517
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	15347	1.500022480300415	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	8829	0.8629503146264653	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	8673	0.8477028065188961	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	7299	0.7134074466483827	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	7132	0.6970847937383567	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5211	0.5093254150547639	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4917	0.4805897266981912	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	3975	0.38851823543325403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	2760	0.26976360498007074	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2531	0.24738104500165184	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	2022	0.1976311627788779	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1890	0.18472942514939625	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1835	0.17935370113711224	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1760	0.17202316839308857	No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1676	0.16381297171978207	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCTATACACATCTGACGCA	1549	0.15139993627323536	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1548	0.15130219583664836	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1463	0.1429942587267549	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGCTGACCCAGCCACTTT	1281	0.12520549926792413	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	1274	0.12452131621181525	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGACTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1258	0.12295746922642355	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGGCAGCCACTTTCGTATGC	1223	0.11953655394587917	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCCTC	1065	0.10409356496513598	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0246305900199195	0.0
2	0.0	0.0	0.0	0.19401476662515957	0.0
3	0.0	0.0	0.0	0.21991598232070983	0.0
4	0.0	0.0	0.0	0.4593800519588161	0.0
5	0.0	0.0	0.0	1.2995568448605146	0.0
6	0.0	0.0	0.0	1.4147928195965667	0.0
7	0.0	0.0	0.0	1.668722473849546	0.0
8	0.0	0.0	0.0	2.2912313144720353	0.0
9	0.0	0.0	0.0	2.4222034994985915	0.0
10	0.0	0.0	0.0	5.275344583909187	0.0
11	0.0	0.0	0.0	5.467600022675781	0.0
12	0.0	0.0	0.0	7.09282800224412	0.0
13	0.0	0.0	0.0	7.241100244546573	0.0
14	0.0	0.0	0.0	7.340795489865294	0.0
15	0.0	0.0	0.0	7.671744608148816	0.0
16	0.0	0.0	0.0	7.862142978620257	0.0
17	0.0	0.0	0.0	7.996145117181009	0.0
18	0.0	0.0	0.0	8.1583942419154	0.0
19	0.0	0.0	0.0	8.583760621941947	0.0
20	0.0	0.0	0.0	8.701440107592672	0.0
21	0.0	0.0	0.0	8.79673703326498	0.0
22	0.0	0.0	0.0	8.927220516108601	0.0
23	0.0	0.0	0.0	9.049982504461852	0.0
24	0.0	0.0	0.0	9.174210599363905	0.0
25	0.0	0.0	0.0	9.252402948633492	0.0
26	0.0	0.0	0.0	9.364022527215825	0.0
27	0.0	0.0	0.0	9.589802935731754	0.0
28	0.0	0.0	0.0	9.669852353296491	0.0
29	0.0	0.0	0.0	9.753909128761297	0.0
30	0.0	0.0	0.0	9.860837166387455	0.0
31	0.0	0.0	0.0	9.95847986253785	0.0
32	0.0	0.0	0.0	10.067167228022575	0.0
33	0.0	0.0	0.0	10.155426842260619	0.0
34	0.0	0.0	0.0	10.271444740489367	0.0
35	0.0	0.0	0.0	10.392056439237702	0.0
36	0.0	0.0	0.0	10.485496296614858	0.0
37	0.0	0.0	0.0	10.589101159397059	0.0
38	0.0	0.0	0.0	10.68332294026691	0.0
39	0.0	0.0	0.0	10.780281453361196	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	7.032924E-4	45.0	22
ATCCGGC	40	6.8139343E-9	45.0	28
ATCCGAT	20	7.032924E-4	45.0	26
ACGTCAA	20	7.032924E-4	45.0	10
CGCGTTC	20	7.032924E-4	45.0	24
CGGTATA	50	2.1827873E-11	45.0	31
CACGTAA	20	7.032924E-4	45.0	1
TGCGTTA	35	1.2117562E-7	45.0	1
TACCGTT	20	7.032924E-4	45.0	13
AATCGCG	25	3.8905047E-5	45.0	21
GTACGAG	45	3.8380676E-10	45.0	2
CTTACGT	25	3.8905047E-5	45.0	41
TACCGCT	45	3.8380676E-10	45.0	29
ACGGTAC	40	6.8139343E-9	45.0	30
CATTGAC	65	0.0	44.999996	26
TGCGCAA	30	2.1652668E-6	44.999996	1
CGTTTTA	3280	0.0	44.79421	1
CGTTATT	780	0.0	44.711536	1
GATGAAT	11170	0.0	44.536705	20
CCGATGA	10890	0.0	44.380165	18
>>END_MODULE