FastQCFastQC Report
Thu 26 May 2016
SRR1546435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546435_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2657020
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208300.7839609788409572No Hit
GAATCTATCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT183740.6915265974663344No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC165220.6218244499476858No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC93450.3517098102385379No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91610.34478475886519483No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG88460.33292937200322165No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA58000.21828966285537935No Hit
GAATATGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT54590.20545573612543375No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG47690.17948679347539725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA302.5289773E-644.030
GTATTCG302.5289773E-644.012
ACGTTGC207.8583485E-444.039
ACGCAAC207.8583485E-444.029
ATTCGTT207.8583485E-444.030
CGCGATA254.4444234E-544.020
TAACGTT207.8583485E-444.035
ACGGGTA600.044.05
TATTCGA700.044.013
CGTACAC254.4444234E-544.034
ACGTCAC207.8583485E-444.021
TCGACTA302.5289773E-644.01
AGTACGC207.8583485E-444.028
CGTTATT76550.043.6263851
CGTTTTA132900.043.304741
GAATCTA22450.042.432071
GTTTTAT139950.042.286532
TAATACG31150.042.234354
AGGCGAT16400.041.8536577
ATAATAC42700.041.7330253