##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546435_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2657020
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.466769915168122	31.0	31.0	33.0	30.0	34.0
2	31.63900648094482	31.0	31.0	34.0	30.0	34.0
3	31.62659370271959	31.0	31.0	34.0	30.0	34.0
4	35.55438009499364	37.0	35.0	37.0	33.0	37.0
5	26.87463398845323	35.0	19.0	37.0	0.0	37.0
6	31.182243265011177	35.0	25.0	37.0	17.0	37.0
7	15.26782372733363	0.0	0.0	32.0	0.0	37.0
8	25.07955604398913	17.0	17.0	35.0	17.0	37.0
9	32.86056484332071	32.0	32.0	37.0	27.0	39.0
10	35.85522201564158	37.0	35.0	39.0	32.0	39.0
11	36.89709900565295	39.0	37.0	39.0	33.0	39.0
12	36.81335556375187	39.0	35.0	39.0	33.0	39.0
13	36.32951200969507	39.0	35.0	39.0	32.0	39.0
14	37.791182603066595	40.0	36.0	41.0	33.0	41.0
15	37.970907633363694	40.0	36.0	41.0	33.0	41.0
16	38.13886007632611	40.0	36.0	41.0	33.0	41.0
17	38.0343843855146	40.0	36.0	41.0	33.0	41.0
18	37.975181594417805	40.0	36.0	41.0	33.0	41.0
19	37.942151357535884	40.0	36.0	41.0	33.0	41.0
20	37.79751225056643	40.0	36.0	41.0	33.0	41.0
21	37.790086638414465	40.0	35.0	41.0	33.0	41.0
22	37.615523029559434	39.0	35.0	41.0	33.0	41.0
23	37.49654199065118	39.0	35.0	41.0	33.0	41.0
24	37.34008626205298	39.0	35.0	41.0	33.0	41.0
25	37.25119570044637	39.0	35.0	40.0	33.0	41.0
26	36.882474350964614	38.0	35.0	40.0	33.0	41.0
27	36.58719693491204	38.0	35.0	40.0	32.0	41.0
28	36.63567229452545	38.0	35.0	40.0	32.0	41.0
29	36.65860475269287	38.0	35.0	40.0	32.0	41.0
30	36.37536412973933	38.0	35.0	40.0	31.0	41.0
31	36.18697262346539	38.0	35.0	40.0	31.0	41.0
32	35.71250122317483	38.0	35.0	40.0	30.0	41.0
33	35.334697141910866	38.0	34.0	40.0	27.0	41.0
34	34.86049747461442	38.0	34.0	40.0	24.0	41.0
35	34.55985013285561	38.0	34.0	40.0	22.0	41.0
36	34.33237198063996	38.0	34.0	40.0	21.0	41.0
37	34.12841002325914	38.0	34.0	40.0	20.0	41.0
38	33.980547380147684	37.0	33.0	40.0	19.0	41.0
39	33.89166848574719	37.0	33.0	40.0	18.0	41.0
40	33.623250107263026	37.0	33.0	40.0	18.0	40.0
41	33.39914678850742	37.0	33.0	40.0	18.0	40.0
42	33.381554523488724	37.0	33.0	40.0	17.0	40.0
43	33.22383534937637	36.0	33.0	40.0	15.0	40.0
44	33.05432778074685	36.0	33.0	40.0	15.0	40.0
45	32.885357656321744	36.0	32.0	39.0	15.0	40.0
46	32.80372898961995	36.0	32.0	39.0	15.0	40.0
47	32.6660220848921	35.0	32.0	39.0	15.0	40.0
48	32.61561824901582	35.0	32.0	39.0	15.0	40.0
49	32.487626363369486	35.0	32.0	39.0	15.0	40.0
50	32.49721003229182	35.0	32.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	8.0
13	9.0
14	16.0
15	50.0
16	190.0
17	576.0
18	1451.0
19	3247.0
20	6129.0
21	11122.0
22	17400.0
23	25383.0
24	37377.0
25	53663.0
26	66616.0
27	67962.0
28	62765.0
29	62137.0
30	69104.0
31	89154.0
32	122184.0
33	218695.0
34	223043.0
35	268301.0
36	326705.0
37	590719.0
38	267171.0
39	65839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.29846218696133	19.798345514900152	25.837592490835597	20.06559980730292
2	33.82134872902726	28.32003522743525	26.33167985186412	11.526936191673379
3	24.98682734793114	27.54345093375285	35.20108994286832	12.268631775447682
4	22.98048189324883	23.431212410896418	40.34290295142679	13.245402744427967
5	19.01859978472123	44.85739663231741	25.214149686490885	10.909853896470482
6	18.585031350911922	39.94275541772361	32.00559273170695	9.46662049965751
7	34.30975303159178	54.62623540658331	10.026458212584023	1.0375533492408788
8	76.13834295564203	7.563699181790126	14.301209625821409	1.9967482367464304
9	72.35376474396128	5.010011215572333	14.434516864758262	8.201707175708124
10	47.746723773249734	21.553018042769718	19.815771051779812	10.884487132200736
11	43.38616194082092	16.441464497820867	26.415909552807282	13.756464008550934
12	36.57992788913896	17.935318514727026	30.090816026977592	15.393937569156424
13	20.902213758270545	34.13613747732422	31.975897810328863	12.985750954076373
14	14.265154195301502	38.75616291936079	32.280901159946104	14.697781725391604
15	12.861363482397573	19.731466078539114	52.369496654146374	15.037673784916938
16	14.126013353305582	17.1588847656397	46.88395269888822	21.831149182166488
17	14.507305176475901	18.889583066743945	34.88069340840491	31.722418348375246
18	19.28024629095754	21.12050342112592	37.277814995747114	22.321435292169422
19	25.560176438265426	21.834649344001928	29.973955785052425	22.63121843268022
20	28.878668583601176	20.919752203596513	31.881769802259676	18.319809410542636
21	20.272786806271686	27.5372409692061	32.24495863787251	19.945013586649704
22	20.41350836651587	21.764006292764073	31.9935868002499	25.828898540470153
23	20.13556540786294	27.286094948476112	31.020504173848902	21.557835469812044
24	19.744977455946888	21.77928656916395	39.972939609035684	18.502796365853474
25	16.17296821250875	26.972510556939728	37.555118139870984	19.299403090680535
26	16.35490135565408	29.05495630443128	33.9578550406094	20.632287299305236
27	19.918329557173074	28.967000624760068	32.642020007376686	18.47264981069017
28	14.002717329941062	26.706460621297545	41.796260472258396	17.494561576503
29	16.588471294909336	21.731300479484535	41.55851292048987	20.121715305116258
30	18.647733174759693	28.68890712151207	35.25159765451521	17.411762049213028
31	26.49611218583225	23.10483172877886	29.81467960346554	20.584376481923357
32	29.695260103424136	24.127067165471093	29.107609276557948	17.070063454546826
33	26.56882522525235	26.035596269504936	27.21360772594862	20.181970779294097
34	18.919767258056016	26.80540605640906	31.958660454193044	22.31616623134188
35	21.930395706468147	24.14844449797141	31.607966819971246	22.313192975589192
36	27.97570210235527	25.05938984275617	29.67798511113955	17.286922943749012
37	20.924117996853617	29.559581787114887	30.27545897283423	19.240841243197266
38	19.596126487568778	31.663291958660455	25.97853986797239	22.762041685798376
39	21.725692693318077	30.823930568832754	27.357603631135635	20.092773106713537
40	22.433779196242405	27.02937877772843	27.829372755944632	22.70746927008453
41	18.604715056717676	25.53910019495525	27.504346975182724	28.35183777314435
42	20.667401826105937	25.378544384310242	28.016311506876125	25.937742282707692
43	22.957260389458867	25.590360629577496	26.703299184801015	24.74907979616262
44	20.424761574997554	28.43012096258214	28.626882748342126	22.51823471407818
45	17.261367998735423	32.94510391340675	25.979668952435436	23.81385913542239
46	22.5880874061919	31.54485099848703	26.101346621402925	19.76571497391815
47	20.5161045080579	26.154338318868508	31.636118659249835	21.693438513823757
48	22.614846707966066	23.606747408751158	30.85904509563345	22.91936078764932
49	20.02224296392199	22.8586160435375	34.27313305884036	22.84600793370016
50	18.867377738970724	29.83823983259441	29.435495404626234	21.858887023808627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1193.0
1	2307.5
2	3422.0
3	32652.0
4	61882.0
5	45039.5
6	28197.0
7	28811.5
8	29426.0
9	30388.5
10	31351.0
11	30766.0
12	30181.0
13	28419.0
14	26657.0
15	24660.5
16	22664.0
17	20989.0
18	19314.0
19	17798.0
20	16282.0
21	16049.0
22	15816.0
23	16931.0
24	18046.0
25	22533.5
26	27021.0
27	30584.0
28	34147.0
29	34738.0
30	35329.0
31	39035.0
32	42741.0
33	48582.5
34	54424.0
35	58494.0
36	62564.0
37	73352.0
38	84140.0
39	113338.0
40	142536.0
41	198319.5
42	254103.0
43	271568.0
44	289033.0
45	258594.0
46	228155.0
47	210099.0
48	192043.0
49	178661.5
50	165280.0
51	157998.5
52	150717.0
53	134867.5
54	119018.0
55	109077.0
56	99136.0
57	96284.0
58	93432.0
59	88219.5
60	83007.0
61	74531.0
62	66055.0
63	56108.5
64	46162.0
65	38132.0
66	30102.0
67	25174.0
68	20246.0
69	17092.0
70	13938.0
71	11573.5
72	9209.0
73	7246.0
74	5283.0
75	3991.0
76	2699.0
77	1966.5
78	1234.0
79	898.5
80	563.0
81	371.0
82	179.0
83	113.5
84	48.0
85	38.0
86	28.0
87	17.5
88	7.0
89	7.0
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2657020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.36396716056599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.44058845218566	16.788174359380445
2	6.0540932200256945	2.465707110392169
3	1.9967527556901	1.2198542263392853
4	0.995698871950202	0.8110551652082606
5	0.6504103289138335	0.6622467289447115
6	0.4861801727559509	0.594033424327229
7	0.3649126064114896	0.5201747833408081
8	0.2996127211273909	0.4881042891140804
9	0.26254428987552536	0.4811798967497385
>10	3.903792238009249	20.1787316154754
>50	1.6516751923266377	24.04015616598064
>100	0.8845890187372335	25.52813187090475
>500	0.00434631269575458	0.677413575623031
>1k	0.0029737928970952395	1.1505130305829747
>5k	0.0011437664988827843	1.7986257439403692
>10k+	6.862598993296707E-4	2.595898013696123
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20830	0.7839609788409572	No Hit
GAATCTATCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	18374	0.6915265974663344	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	16522	0.6218244499476858	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	9345	0.3517098102385379	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9161	0.34478475886519483	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG	8846	0.33292937200322165	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5800	0.21828966285537935	No Hit
GAATATGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	5459	0.20545573612543375	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG	4769	0.17948679347539725	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01742553687966218	0.0
2	0.0	0.0	0.0	0.08129408133924472	0.0
3	0.0	0.0	0.0	0.10007451957456097	0.0
4	0.0	0.0	0.0	0.19469179757773747	0.0
5	0.0	0.0	0.0	0.5688703886308721	0.0
6	0.0	0.0	0.0	0.6785797622900844	0.0
7	0.0	0.0	0.0	0.8261134654613063	0.0
8	0.0	0.0	0.0	1.2138410700709819	0.0
9	0.0	0.0	0.0	1.3261473379951976	0.0
10	0.0	0.0	0.0	2.8895905939736997	0.0
11	0.0	0.0	0.0	3.0615877938442315	0.0
12	0.0	0.0	0.0	4.005163679610993	0.0
13	0.0	0.0	0.0	4.1146848725263645	0.0
14	0.0	0.0	0.0	4.183709569367186	0.0
15	0.0	0.0	0.0	4.357287487485981	0.0
16	0.0	0.0	0.0	4.505159915996116	0.0
17	0.0	0.0	0.0	4.64279531204131	0.0
18	0.0	0.0	0.0	4.85242866068001	0.0
19	0.0	0.0	0.0	5.028866926105185	0.0
20	0.0	0.0	0.0	5.107865202369572	0.0
21	0.0	0.0	0.0	5.182158960037937	0.0
22	0.0	0.0	0.0	5.27850750088445	0.0
23	0.0	0.0	0.0	5.36687717819211	0.0
24	0.0	0.0	0.0	5.441923658835838	0.0
25	0.0	0.0	0.0	5.496646619144756	0.0
26	0.0	0.0	0.0	5.569284386267322	0.0
27	0.0	0.0	0.0	5.672557978487178	0.0
28	0.0	0.0	0.0	5.733377994896538	0.0
29	0.0	0.0	0.0	5.793482924479304	0.0
30	0.0	0.0	0.0	5.871013390941732	0.0
31	0.0	0.0	0.0	5.942860798940166	0.0
32	0.0	0.0	0.0	6.0155362022115	0.0
33	0.0	0.0	0.0	6.0813618264070275	0.0
34	0.0	0.0	0.0	6.168000240871352	0.0
35	0.0	0.0	0.0	6.266268225305041	0.0
36	0.0	0.0	0.0	6.345567590759573	0.0
37	0.0	0.0	0.0	6.42373787175106	0.0
38	0.0	0.0	0.0	6.4981069017169615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	30	2.5289773E-6	44.0	30
GTATTCG	30	2.5289773E-6	44.0	12
ACGTTGC	20	7.8583485E-4	44.0	39
ACGCAAC	20	7.8583485E-4	44.0	29
ATTCGTT	20	7.8583485E-4	44.0	30
CGCGATA	25	4.4444234E-5	44.0	20
TAACGTT	20	7.8583485E-4	44.0	35
ACGGGTA	60	0.0	44.0	5
TATTCGA	70	0.0	44.0	13
CGTACAC	25	4.4444234E-5	44.0	34
ACGTCAC	20	7.8583485E-4	44.0	21
TCGACTA	30	2.5289773E-6	44.0	1
AGTACGC	20	7.8583485E-4	44.0	28
CGTTATT	7655	0.0	43.626385	1
CGTTTTA	13290	0.0	43.30474	1
GAATCTA	2245	0.0	42.43207	1
GTTTTAT	13995	0.0	42.28653	2
TAATACG	3115	0.0	42.23435	4
AGGCGAT	1640	0.0	41.853657	7
ATAATAC	4270	0.0	41.733025	3
>>END_MODULE