FastQCFastQC Report
Thu 26 May 2016
SRR1546434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546434_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1116309
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG100410.8994821326353187No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG61420.5502060809327883No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57190.5123133469317187No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC55570.49780123603769205No Hit
GAATCTATCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC54240.48588697215555904No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51860.46456671047174214No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC49740.445575553005485No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC28700.25709727324602777No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT24470.21920453924495817No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22960.20567781859682222No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA12880.11538023970065636No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT11250.1007785478751851No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT11230.10059938601229587No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.0331985E-445.0000042
CTCGTAG207.0331985E-445.0000042
CGAGTCA207.0331985E-445.00000434
ACGTTGC207.0331985E-445.00000439
TTAGATC302.165445E-645.00000413
CTAAACG207.0331985E-445.00000445
GACGGTC600.045.00000429
CGTACTG207.0331985E-445.00000413
ATGCGTA207.0331985E-445.00000411
CGGTATA207.0331985E-445.00000431
TAAATCG207.0331985E-445.0000041
CGGTAAG302.165445E-645.0000042
TACGTAA207.0331985E-445.0000041
TATTTCG253.890734E-545.020
CGTATTA253.890734E-545.014
CGACGGT502.1827873E-1145.028
TGTTGCG253.890734E-545.01
CCCGTAA253.890734E-545.041
TCGGAAT253.890734E-545.024
ACTCGAT351.2118835E-745.020