##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546434_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1116309
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.881951144351607	31.0	30.0	31.0	30.0	33.0
2	30.992539700029294	31.0	31.0	33.0	28.0	34.0
3	30.912825212373992	31.0	30.0	33.0	28.0	34.0
4	34.9299405451358	35.0	35.0	37.0	32.0	37.0
5	32.81802529586342	35.0	35.0	37.0	28.0	37.0
6	33.937316639030946	35.0	35.0	37.0	29.0	37.0
7	17.559526081040286	25.0	0.0	35.0	0.0	37.0
8	25.76597429564753	23.0	17.0	35.0	17.0	37.0
9	32.69540960432998	32.0	32.0	37.0	27.0	39.0
10	35.25605455120401	37.0	34.0	37.0	32.0	39.0
11	36.21481238617623	37.0	35.0	39.0	32.0	39.0
12	35.923776481243095	37.0	35.0	39.0	31.0	39.0
13	35.52616255893305	37.0	35.0	39.0	30.0	39.0
14	36.73319484121332	38.0	35.0	40.0	31.0	41.0
15	37.04384897013282	39.0	35.0	40.0	31.0	41.0
16	37.28974593952033	39.0	35.0	40.0	32.0	41.0
17	36.65833564004232	38.0	35.0	40.0	30.0	41.0
18	36.77922958607339	38.0	35.0	40.0	31.0	41.0
19	36.77161610270991	38.0	35.0	40.0	31.0	41.0
20	36.20986035228597	38.0	34.0	40.0	29.0	41.0
21	36.41370803245338	38.0	35.0	40.0	30.0	41.0
22	36.24357861488172	38.0	35.0	40.0	30.0	41.0
23	36.069806836637525	38.0	35.0	40.0	30.0	41.0
24	36.163874876938195	38.0	34.0	40.0	30.0	41.0
25	36.282551694916016	38.0	35.0	40.0	30.0	41.0
26	35.90977139842105	38.0	34.0	40.0	30.0	41.0
27	35.353177301266946	37.0	34.0	40.0	27.0	41.0
28	35.25953297877201	37.0	34.0	40.0	27.0	41.0
29	35.444432500320254	37.0	34.0	40.0	27.0	41.0
30	35.04220605585013	37.0	34.0	40.0	25.0	41.0
31	35.06790951250953	37.0	34.0	40.0	25.0	41.0
32	34.22743792265403	37.0	33.0	40.0	22.0	41.0
33	33.796171131828196	37.0	33.0	40.0	20.0	41.0
34	33.06489690578505	37.0	32.0	40.0	13.0	41.0
35	32.62279082225441	37.0	32.0	40.0	10.0	41.0
36	32.38841754388794	37.0	31.0	40.0	11.0	41.0
37	32.254882832620716	36.0	31.0	40.0	11.0	41.0
38	32.22897602724694	36.0	30.0	40.0	10.0	41.0
39	32.13670139719379	36.0	30.0	40.0	11.0	41.0
40	31.978099253880423	36.0	30.0	40.0	10.0	41.0
41	31.825350328627646	36.0	30.0	40.0	10.0	41.0
42	31.68627145351332	35.0	30.0	40.0	9.0	41.0
43	31.455947233248143	35.0	29.0	40.0	8.0	41.0
44	31.558810329398042	35.0	29.0	40.0	10.0	41.0
45	31.612507827133886	35.0	29.0	40.0	10.0	41.0
46	31.474724292288247	35.0	29.0	40.0	10.0	41.0
47	31.485642416212716	35.0	29.0	40.0	10.0	40.0
48	31.66758397540466	35.0	29.0	40.0	10.0	41.0
49	31.564290890783823	35.0	29.0	39.0	10.0	40.0
50	31.17498559986527	35.0	28.0	39.0	9.0	40.0
51	31.147438567636737	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	9.0
14	30.0
15	58.0
16	170.0
17	492.0
18	1209.0
19	2949.0
20	6492.0
21	12206.0
22	18091.0
23	23315.0
24	28852.0
25	33831.0
26	36339.0
27	34281.0
28	31665.0
29	30983.0
30	33508.0
31	41187.0
32	53988.0
33	77842.0
34	96738.0
35	100294.0
36	110988.0
37	163564.0
38	147555.0
39	29669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86503736868555	18.841736472607494	23.977321691395485	17.315904467311473
2	29.955505151351463	34.76340332291507	24.574109856679467	10.706981669053999
3	23.38465424895795	33.824505580444125	31.41567433389859	11.375165836699336
4	21.60315826531901	22.091105598897794	43.939536454512144	12.366199681271047
5	30.39803495268783	29.688912299372305	27.49821062089439	12.414842127045468
6	17.163168979198414	45.7722727309374	28.44131866714324	8.623239622720948
7	30.891894627741962	50.15161572647	18.15832354661657	0.798166099171466
8	70.48872668768236	15.555549583493459	12.02534423712431	1.9303794916998789
9	66.92179315942091	4.770184599425428	12.093157002227878	16.214865238925782
10	52.17757807202128	20.277539641801688	17.25095829201413	10.293923994162906
11	48.40675834379191	15.349423860239414	23.513202885580963	12.730614910387716
12	34.24687967220545	16.9091174576215	26.689832295538242	22.1541705746348
13	27.176525496076803	32.20174700732503	28.725111057959758	11.896616438638405
14	13.21892056769228	44.34282980787578	28.570136046560584	13.86811357787136
15	11.77057606809584	18.59942005304983	47.998448458267376	21.631555420586952
16	12.958777542777133	16.429949055324286	42.289455697302444	28.321817704596132
17	13.283508419263843	25.56165004492484	31.524515165603788	29.630326370207534
18	17.770975599050086	20.140928721348658	33.88371857612901	28.204377103472243
19	23.948566212401765	20.79710904418042	26.59156201374351	28.662762729674313
20	34.39522569467773	19.723033676159556	29.03936096546745	16.84237966369527
21	18.985603448507536	33.84699039423672	28.627736585479468	18.539669571776273
22	18.924688415125203	20.627353179092886	35.56999002964233	24.87796837613958
23	25.834871885830896	26.14070118578279	27.93993419384776	20.084492734538557
24	18.11335391903138	27.90257894543536	36.48201349267989	17.50205364285337
25	14.784616087481153	33.43966589895808	33.89034756505591	17.88537044850485
26	15.018153575757252	27.45610758311543	38.2624345051415	19.263304335985826
27	25.83236361975045	27.714011084744456	29.104038397970456	17.349586897534643
28	12.793948628919056	32.69121721673838	38.30812078017825	16.206713374164323
29	15.66985485201678	20.424631531233736	45.09423466083315	18.81127895591633
30	17.308021345344347	34.93862362482073	31.61526064915718	16.13809438067775
31	24.92535668887378	21.780886833305114	26.610553171209762	26.683203306611343
32	35.30187430182862	22.69407484845146	26.192299802294883	15.811751047425041
33	31.9356020600031	24.372552760929096	24.101122538651932	19.59072264041587
34	18.67081605541118	25.185947618446146	29.638388654037545	26.504847672105125
35	25.48120636848758	22.869474312220003	29.608647784797938	22.040671534494482
36	26.615659284302108	28.32145938087035	27.728791938432817	17.334089396394724
37	20.727773403242292	28.91439556610222	29.766220643209003	20.591610387446487
38	18.74570571409887	31.215371371188443	24.192674250588322	25.84624866412436
39	20.517616538073238	31.89439483153858	25.16229825254477	22.42569037784341
40	21.165286672417764	26.92883422063246	25.41007910892056	26.495799998029216
41	17.893611894197754	24.931627354074905	24.97202835415642	32.20273239757092
42	19.4921836158268	25.985278269726393	26.531811532469952	27.990726581976855
43	22.582725750665812	25.166239813528335	25.863985688550393	26.387048747255466
44	20.493429686583195	26.993959557792692	28.594412478982072	23.91819827664204
45	16.803680701311198	31.80535138568264	26.105047975067837	25.285919937938328
46	24.22116098678771	31.276465566433664	24.32480612446912	20.177567322309503
47	19.482956779888006	28.72072159231897	30.5273002367624	21.26902139103062
48	21.685035236659385	24.711258262721163	30.071602038503677	23.532104462115775
49	20.79173418829374	23.033138673969304	33.54393810315961	22.631189034577343
50	19.66614978469223	29.876226027022984	28.29915372894064	22.158470459344144
51	18.32019628973698	29.181436322738598	28.13754972861457	24.360817658909852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	510.0
1	873.5
2	1237.0
3	10516.0
4	19795.0
5	14644.0
6	9493.0
7	9686.5
8	9880.0
9	10069.5
10	10259.0
11	10103.0
12	9947.0
13	9390.0
14	8833.0
15	8197.5
16	7562.0
17	7097.0
18	6632.0
19	6225.5
20	5819.0
21	5776.5
22	5734.0
23	6115.5
24	6497.0
25	7810.5
26	10804.5
27	12485.0
28	12993.5
29	13502.0
30	14563.5
31	15625.0
32	18436.5
33	21248.0
34	23337.0
35	25426.0
36	27641.5
37	29857.0
38	38454.0
39	47051.0
40	60865.0
41	74679.0
42	85911.0
43	97143.0
44	110925.5
45	124708.0
46	103602.5
47	82497.0
48	78065.5
49	73634.0
50	72892.0
51	72150.0
52	70431.5
53	68713.0
54	64082.5
55	59452.0
56	55642.5
57	51833.0
58	46569.0
59	41305.0
60	35952.5
61	30600.0
62	26305.5
63	22011.0
64	18452.0
65	14893.0
66	12125.0
67	9357.0
68	7736.5
69	6116.0
70	5306.5
71	4497.0
72	3717.0
73	2937.0
74	2301.0
75	1268.5
76	872.0
77	645.5
78	419.0
79	307.0
80	195.0
81	136.0
82	77.0
83	47.5
84	18.0
85	16.5
86	15.0
87	8.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1116309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.367499189383146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80313206416703	19.990383887586106
2	5.392281883404883	2.7357741261239763
3	2.271495838603018	1.7286650653334776
4	1.4061022534340468	1.42677191096712
5	1.0529598910037992	1.335547959074592
6	0.8051868028110306	1.2255345340566497
7	0.7241291065020272	1.2858541165539243
8	0.595140439075054	1.2077779684644452
9	0.5583333130990233	1.2747167880700576
>10	7.439166897272016	43.8772445586857
>50	0.8466505708718739	13.684560120919977
>100	0.09228891957899392	3.6135898302561515
>500	0.006566010088624019	1.106442692898327
>1k	0.004377340059082679	1.9844161201977604
>5k	0.00182389169128445	2.593574959986143
>10k+	3.6477833825688996E-4	0.9291453608256336
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	10041	0.8994821326353187	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	6142	0.5502060809327883	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5719	0.5123133469317187	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	5557	0.49780123603769205	No Hit
GAATCTATCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	5424	0.48588697215555904	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5186	0.46456671047174214	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	4974	0.445575553005485	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	2870	0.25709727324602777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT	2447	0.21920453924495817	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2296	0.20567781859682222	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA	1288	0.11538023970065636	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	1125	0.1007785478751851	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1123	0.10059938601229587	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018901576534812493	0.0
2	0.0	0.0	0.0	0.1098262219510906	0.0
3	0.0	0.0	0.0	0.13025067432046145	0.0
4	0.0	0.0	0.0	0.2625617100641489	0.0
5	0.0	0.0	0.0	0.8000472987318028	0.0
6	0.0	0.0	0.0	0.8842533742897352	0.0
7	0.0	0.0	0.0	1.0307181972016708	0.0
8	0.0	0.0	0.0	1.4126912888814835	0.0
9	0.0	0.0	0.0	1.5121261227849996	0.0
10	0.0	0.0	0.0	3.2750788536149043	0.0
11	0.0	0.0	0.0	3.4135709736282696	0.0
12	0.0	0.0	0.0	4.415085787178998	0.0
13	0.0	0.0	0.0	4.520343381626414	0.0
14	0.0	0.0	0.0	4.589141536975873	0.0
15	0.0	0.0	0.0	4.796163069544365	0.0
16	0.0	0.0	0.0	4.947644424617199	0.0
17	0.0	0.0	0.0	5.070280719764868	0.0
18	0.0	0.0	0.0	5.250517553831421	0.0
19	0.0	0.0	0.0	5.587610598857485	0.0
20	0.0	0.0	0.0	5.671547931621084	0.0
21	0.0	0.0	0.0	5.733000450592085	0.0
22	0.0	0.0	0.0	5.821954315516582	0.0
23	0.0	0.0	0.0	5.897291878861498	0.0
24	0.0	0.0	0.0	5.972181537549191	0.0
25	0.0	0.0	0.0	6.017957393517387	0.0
26	0.0	0.0	0.0	6.093115794999413	0.0
27	0.0	0.0	0.0	6.207510644454179	0.0
28	0.0	0.0	0.0	6.257944708857494	0.0
29	0.0	0.0	0.0	6.3090954207123655	0.0
30	0.0	0.0	0.0	6.372518720175148	0.0
31	0.0	0.0	0.0	6.433612915420372	0.0
32	0.0	0.0	0.0	6.4999923856208275	0.0
33	0.0	0.0	0.0	6.553113877967481	0.0
34	0.0	0.0	0.0	6.638126181908414	0.0
35	0.0	0.0	0.0	6.722063514672013	0.0
36	0.0	0.0	0.0	6.790413765364249	0.0
37	0.0	0.0	0.0	6.860555634685379	0.0
38	0.0	0.0	0.0	6.924874743462608	0.0
39	0.0	0.0	0.0	6.986327262433609	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.0331985E-4	45.000004	2
CTCGTAG	20	7.0331985E-4	45.000004	2
CGAGTCA	20	7.0331985E-4	45.000004	34
ACGTTGC	20	7.0331985E-4	45.000004	39
TTAGATC	30	2.165445E-6	45.000004	13
CTAAACG	20	7.0331985E-4	45.000004	45
GACGGTC	60	0.0	45.000004	29
CGTACTG	20	7.0331985E-4	45.000004	13
ATGCGTA	20	7.0331985E-4	45.000004	11
CGGTATA	20	7.0331985E-4	45.000004	31
TAAATCG	20	7.0331985E-4	45.000004	1
CGGTAAG	30	2.165445E-6	45.000004	2
TACGTAA	20	7.0331985E-4	45.000004	1
TATTTCG	25	3.890734E-5	45.0	20
CGTATTA	25	3.890734E-5	45.0	14
CGACGGT	50	2.1827873E-11	45.0	28
TGTTGCG	25	3.890734E-5	45.0	1
CCCGTAA	25	3.890734E-5	45.0	41
TCGGAAT	25	3.890734E-5	45.0	24
ACTCGAT	35	1.2118835E-7	45.0	20
>>END_MODULE