FastQCFastQC Report
Thu 26 May 2016
SRR1546433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546433_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1332230
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71840.5392462262522237No Hit
GAATCTATCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT49250.3696809109538143No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44520.33417653107946826No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC33700.252959323840478No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.22713795665913541No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.2244357205587624No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG26460.1986143533774198No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT23370.17542016018255105No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC21130.1586062466691187No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC19080.14321851331977212No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG17490.1312836372097911No Hit
GAATATGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT14690.11026624531800064No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG14200.10658820173693731No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG254.4432236E-544.01
GTCGAAA207.856934E-444.01
CGTTGCG207.856934E-444.012
TGTGATC254.4432236E-544.042
CACTCGA351.4463149E-744.025
TGCGATA900.044.01
CGCTAAT302.5280224E-644.021
CGCTAAG207.856934E-444.02
GAACGTA254.4432236E-544.044
GTATCGA207.856934E-444.09
ACGTCGA408.314601E-944.011
AATAGTA207.856934E-444.01
CACGTAT254.4432236E-544.030
GCTGTAT254.4432236E-544.02
ACGAGCC302.5280224E-644.029
CCGTTGC207.856934E-444.011
GCCCGTA207.856934E-444.027
GTCGGAT254.4432236E-544.040
ATTTCGC454.802132E-1044.011
TCTATAC408.314601E-944.028
Produced by FastQC (version 0.11.5)