##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546433_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1332230
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447642674312995	31.0	31.0	33.0	30.0	34.0
2	31.631998979155252	31.0	31.0	34.0	30.0	34.0
3	31.667279673930178	31.0	31.0	34.0	30.0	34.0
4	35.5686045202405	37.0	35.0	37.0	33.0	37.0
5	27.055919022991525	35.0	25.0	37.0	0.0	37.0
6	31.276392965178687	35.0	26.0	37.0	17.0	37.0
7	15.060626168154148	0.0	0.0	32.0	0.0	37.0
8	25.010052318293386	17.0	17.0	35.0	17.0	37.0
9	32.91722450327646	32.0	32.0	37.0	27.0	39.0
10	35.902100237946904	37.0	35.0	39.0	32.0	39.0
11	36.91834668187926	39.0	37.0	39.0	33.0	39.0
12	36.794515211337384	39.0	35.0	39.0	33.0	39.0
13	36.23900602748775	38.0	35.0	39.0	32.0	39.0
14	37.59744788812743	40.0	36.0	41.0	33.0	41.0
15	37.83338162329328	40.0	36.0	41.0	33.0	41.0
16	37.9988275297809	40.0	36.0	41.0	33.0	41.0
17	37.962543254543135	40.0	36.0	41.0	33.0	41.0
18	37.94657529105335	40.0	36.0	41.0	33.0	41.0
19	37.90999301922341	40.0	36.0	41.0	34.0	41.0
20	37.78015432770618	40.0	35.0	41.0	33.0	41.0
21	37.69639401604828	40.0	35.0	41.0	33.0	41.0
22	37.55578541242878	39.0	35.0	41.0	33.0	41.0
23	37.46326610269998	39.0	35.0	41.0	33.0	41.0
24	37.29979658167133	39.0	35.0	41.0	33.0	41.0
25	37.17773807825976	39.0	35.0	41.0	33.0	41.0
26	36.840675408900864	38.0	35.0	40.0	32.0	41.0
27	36.563176778784445	38.0	35.0	40.0	32.0	41.0
28	36.612181830464706	38.0	35.0	40.0	32.0	41.0
29	36.60492932901976	38.0	35.0	40.0	32.0	41.0
30	36.36731645436599	38.0	35.0	40.0	31.0	41.0
31	36.2077058766129	38.0	35.0	40.0	31.0	41.0
32	35.81908078935319	38.0	35.0	40.0	30.0	41.0
33	35.50777268189427	38.0	34.0	40.0	29.0	41.0
34	35.111300601247535	38.0	34.0	40.0	26.0	41.0
35	34.8003407819971	38.0	34.0	40.0	24.0	41.0
36	34.606013225944466	38.0	34.0	40.0	23.0	41.0
37	34.383900677810885	38.0	34.0	40.0	23.0	41.0
38	34.259955863477025	37.0	33.0	40.0	23.0	41.0
39	34.19230538270419	37.0	33.0	40.0	22.0	41.0
40	33.96203733589545	37.0	33.0	40.0	21.0	40.0
41	33.81598222529143	37.0	33.0	40.0	21.0	40.0
42	33.75329935521644	37.0	33.0	40.0	20.0	40.0
43	33.580700029274226	36.0	33.0	40.0	18.0	40.0
44	33.41382043641113	36.0	33.0	40.0	18.0	40.0
45	33.18110461406814	36.0	33.0	39.0	18.0	40.0
46	33.137858327766224	36.0	33.0	39.0	18.0	40.0
47	33.02636706874939	35.0	32.0	39.0	18.0	40.0
48	32.973368712609684	35.0	32.0	39.0	18.0	40.0
49	32.8585904836252	35.0	32.0	39.0	18.0	40.0
50	32.8608678681609	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	12.0
14	15.0
15	31.0
16	88.0
17	265.0
18	708.0
19	1493.0
20	2799.0
21	4842.0
22	7879.0
23	11595.0
24	16839.0
25	23553.0
26	29694.0
27	30884.0
28	29735.0
29	30826.0
30	35758.0
31	46005.0
32	63258.0
33	117541.0
34	114652.0
35	136714.0
36	164800.0
37	297424.0
38	134117.0
39	30697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.1331376714231	22.517733424408696	27.72516757616928	18.62396132799892
2	31.048917979628143	28.154522867673	29.117794975342093	11.678764177356763
3	26.753713698085164	25.97066572588817	34.85817013578736	12.417450440239298
4	23.462540252058577	25.958055290753094	36.99488827004346	13.584516187144862
5	18.451843900827935	46.174008992441244	24.66203283216862	10.7121142745622
6	19.392747498555053	40.55936287277722	30.498937871088327	9.548951757579397
7	35.498224781006286	55.84808929389069	7.581123379596616	1.0725625455064065
8	80.01396155318527	6.541513102091981	11.380317212493338	2.0642081322294197
9	76.04347597637044	5.485689407985108	12.440419447092468	6.0304151685519765
10	53.44565127643125	20.280206871185907	16.64292201796987	9.631219834412978
11	48.7769379161256	18.066925380752572	21.4444202577633	11.711716445358535
12	41.3635032989799	18.93982270328697	26.000315260878377	13.696358736854746
13	22.096785089661694	37.5922325724537	26.53175502728508	13.77922731059952
14	15.16254700764883	42.16816915997988	28.851099284658055	13.818184547713233
15	13.676842587240943	21.13028531109493	51.242803419829904	13.95006868183422
16	14.276213566726467	16.49227235537407	48.40380414793242	20.827709929967046
17	14.723808951907705	18.852375340594342	31.75787964540657	34.665936062091376
18	19.45377299715515	22.638733552014294	35.97742131613911	21.930072134691457
19	28.693543907583525	22.093031983966732	27.64500123852488	21.568422869924863
20	32.20877776359938	21.17757444285146	27.280949986113505	19.332697807435654
21	21.577880696276168	28.748189126502183	28.62253514783483	21.051395029386818
22	21.007333568527955	23.35520142918265	28.628465054832873	27.00899994745652
23	20.71917011326873	29.617408405455514	27.019583705516315	22.64383777575944
24	20.908101453953147	22.333305810558237	39.230313084077075	17.528279651411545
25	16.955630784474153	25.70629696073501	37.14088408157751	20.197188173213334
26	16.40324868828956	32.06098046133175	31.251285438700528	20.284485411678162
27	19.088971123604782	32.23174677045255	30.229164633734417	18.45011747220825
28	15.143556292832319	28.278975852517956	40.19838916703572	16.379078687614
29	16.74147857351959	23.06823896774581	39.11621867095021	21.074063787784393
30	18.2647140508771	30.108990189381714	33.48918730249281	18.137108457248374
31	29.550077689287885	23.7711956644123	25.898380910203194	20.78034573609662
32	31.630424176005643	25.489817824249567	26.580995774002986	16.2987622257418
33	28.644828595662915	25.567281925793594	26.06644498322362	19.721444495319876
34	20.82035384280492	24.75233255518942	29.29546699894162	25.131846603064034
35	20.82553312866397	25.192496791094634	31.26292006635491	22.71905001388649
36	29.83681496438303	25.891325071496663	25.826996839885002	18.444863124235304
37	20.512148803134593	31.63387703324501	28.671700832438844	19.182273331181555
38	21.22186109005202	31.405688206991282	24.53172500243952	22.84072570051718
39	21.96467576919901	30.566343649369855	25.948897712857388	21.520082868573745
40	23.243509003700563	24.888194981347063	27.202059704405396	24.666236310546978
41	16.887699571395327	24.647695968413863	27.004346096394766	31.46025836379604
42	22.16246443932354	25.08808539066077	25.110754149058344	27.638696020957344
43	22.18430751446822	26.411730707160174	25.740225036217467	25.663736742154132
44	20.857359464957252	29.27294836477185	28.364621724477008	21.505070445793894
45	18.15234606636992	34.969487250699956	23.20650338154823	23.671663301381894
46	22.091830990144345	32.6580245152864	25.021430233518238	20.22871426105102
47	22.38599941451551	25.690458854702264	28.628314930605075	23.295226800177147
48	23.474700314510258	22.3571755627782	31.14604835501377	23.02207576769777
49	20.573099239620788	23.06726316026512	33.072742694579766	23.286894905534332
50	18.829931768538465	31.5345698565563	27.12834870855633	22.5071496663489
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	523.0
1	929.0
2	1335.0
3	11774.0
4	22213.0
5	16564.5
6	10916.0
7	11337.0
8	11758.0
9	12285.5
10	12813.0
11	12734.0
12	12655.0
13	11996.0
14	11337.0
15	10676.0
16	10015.0
17	9304.0
18	8593.0
19	8202.0
20	7811.0
21	7428.0
22	7045.0
23	7298.0
24	7551.0
25	9251.0
26	10951.0
27	13037.0
28	15123.0
29	16456.0
30	17789.0
31	20098.5
32	22408.0
33	23189.0
34	23970.0
35	24608.0
36	25246.0
37	33041.5
38	40837.0
39	61721.5
40	82606.0
41	99238.5
42	115871.0
43	119259.5
44	122648.0
45	123337.0
46	124026.0
47	118483.5
48	112941.0
49	103568.5
50	94196.0
51	87432.5
52	80669.0
53	71902.5
54	63136.0
55	60039.0
56	56942.0
57	54240.5
58	51539.0
59	48378.5
60	45218.0
61	39672.0
62	34126.0
63	28092.5
64	22059.0
65	19042.5
66	16026.0
67	13533.0
68	11040.0
69	9307.0
70	7574.0
71	6091.5
72	4609.0
73	3783.0
74	2957.0
75	2295.0
76	1633.0
77	1200.5
78	768.0
79	602.5
80	437.0
81	322.5
82	208.0
83	144.0
84	80.0
85	51.0
86	22.0
87	15.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1332230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.989304865582056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61756067816683	16.501279488624398
2	6.121377365528201	2.569669114446899
3	2.18122060456044	1.3734691274462485
4	1.2861202816167345	1.0797908273864758
5	0.8645237579821687	0.9072876359913209
6	0.6754730315513238	0.8506625632625797
7	0.5677853980335502	0.8342194572286428
8	0.4988115947100884	0.8375766905485762
9	0.4364244357676179	0.824422097980455
>10	6.696279292712533	33.991578115541834
>50	1.5701966569090615	22.868550898704267
>100	0.47580556502733296	13.636559770700933
>500	0.00267951645630839	0.3486150197675743
>1k	0.00535903291261678	2.7991239164974693
>5k	3.8278806518691284E-4	0.5771952758723793
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7184	0.5392462262522237	No Hit
GAATCTATCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	4925	0.3696809109538143	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4452	0.33417653107946826	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	3370	0.252959323840478	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3026	0.22713795665913541	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2990	0.2244357205587624	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	2646	0.1986143533774198	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	2337	0.17542016018255105	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	2113	0.1586062466691187	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	1908	0.14321851331977212	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	1749	0.1312836372097911	No Hit
GAATATGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	1469	0.11026624531800064	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	1420	0.10658820173693731	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013961553185260803	0.0
2	0.0	0.0	0.0	0.04788962866772254	0.0
3	0.0	0.0	0.0	0.06335242413096838	0.0
4	0.0	0.0	0.0	0.13353550062676864	0.0
5	0.0	0.0	0.0	0.36510212200595993	0.0
6	0.0	0.0	0.0	0.47799554131043437	0.0
7	0.0	0.0	0.0	0.583457811338883	0.0
8	0.0	0.0	0.0	1.0344309916455867	0.0
9	0.0	0.0	0.0	1.2091755928030445	0.0
10	0.0	0.0	0.0	2.0548253679920134	0.0
11	0.0	0.0	0.0	2.2580185103172874	0.0
12	0.0	0.0	0.0	2.9364298957387236	0.0
13	0.0	0.0	0.0	3.051274930004579	0.0
14	0.0	0.0	0.0	3.1053947141259393	0.0
15	0.0	0.0	0.0	3.2221163012392755	0.0
16	0.0	0.0	0.0	3.3411648138834886	0.0
17	0.0	0.0	0.0	3.4464769596841385	0.0
18	0.0	0.0	0.0	3.6029814671640783	0.0
19	0.0	0.0	0.0	3.718426998341127	0.0
20	0.0	0.0	0.0	3.800620013060808	0.0
21	0.0	0.0	0.0	3.8872416925005444	0.0
22	0.0	0.0	0.0	3.9771661049518476	0.0
23	0.0	0.0	0.0	4.072870300173394	0.0
24	0.0	0.0	0.0	4.149133407895033	0.0
25	0.0	0.0	0.0	4.219916981302028	0.0
26	0.0	0.0	0.0	4.2813928525855145	0.0
27	0.0	0.0	0.0	4.379048662768441	0.0
28	0.0	0.0	0.0	4.44037440982413	0.0
29	0.0	0.0	0.0	4.50650413216937	0.0
30	0.0	0.0	0.0	4.595903109823379	0.0
31	0.0	0.0	0.0	4.674718329417593	0.0
32	0.0	0.0	0.0	4.764567679754998	0.0
33	0.0	0.0	0.0	4.83955473154035	0.0
34	0.0	0.0	0.0	4.930980386269638	0.0
35	0.0	0.0	0.0	5.042597749637825	0.0
36	0.0	0.0	0.0	5.1277181867995765	0.0
37	0.0	0.0	0.0	5.225073748526906	0.0
38	0.0	0.0	0.0	5.30899319186627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	4.4432236E-5	44.0	1
GTCGAAA	20	7.856934E-4	44.0	1
CGTTGCG	20	7.856934E-4	44.0	12
TGTGATC	25	4.4432236E-5	44.0	42
CACTCGA	35	1.4463149E-7	44.0	25
TGCGATA	90	0.0	44.0	1
CGCTAAT	30	2.5280224E-6	44.0	21
CGCTAAG	20	7.856934E-4	44.0	2
GAACGTA	25	4.4432236E-5	44.0	44
GTATCGA	20	7.856934E-4	44.0	9
ACGTCGA	40	8.314601E-9	44.0	11
AATAGTA	20	7.856934E-4	44.0	1
CACGTAT	25	4.4432236E-5	44.0	30
GCTGTAT	25	4.4432236E-5	44.0	2
ACGAGCC	30	2.5280224E-6	44.0	29
CCGTTGC	20	7.856934E-4	44.0	11
GCCCGTA	20	7.856934E-4	44.0	27
GTCGGAT	25	4.4432236E-5	44.0	40
ATTTCGC	45	4.802132E-10	44.0	11
TCTATAC	40	8.314601E-9	44.0	28
>>END_MODULE