##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546432_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	540866
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.898854799525207	31.0	31.0	31.0	30.0	33.0
2	31.011559240181487	31.0	31.0	33.0	30.0	34.0
3	30.974844046399664	31.0	30.0	34.0	28.0	34.0
4	34.964534653685014	35.0	35.0	37.0	32.0	37.0
5	32.80933909692974	35.0	35.0	37.0	28.0	37.0
6	33.93147655796445	35.0	35.0	37.0	28.0	37.0
7	16.65319506125362	0.0	0.0	35.0	0.0	37.0
8	25.38467014010864	17.0	17.0	35.0	17.0	37.0
9	32.64218678933414	32.0	32.0	37.0	27.0	39.0
10	35.306029589584114	37.0	34.0	37.0	32.0	39.0
11	36.25710989413274	37.0	35.0	39.0	32.0	39.0
12	35.967385637107895	37.0	35.0	39.0	31.0	39.0
13	35.497467025104186	37.0	35.0	39.0	30.0	39.0
14	36.59583519762751	38.0	35.0	40.0	31.0	41.0
15	36.96902005302607	39.0	35.0	40.0	31.0	41.0
16	37.194963262619574	39.0	35.0	40.0	32.0	41.0
17	36.75402225320135	38.0	35.0	40.0	31.0	41.0
18	36.86700032910185	38.0	35.0	40.0	31.0	41.0
19	36.8689638468678	38.0	35.0	40.0	31.0	41.0
20	36.44018666360984	38.0	35.0	40.0	30.0	41.0
21	36.482193371371096	38.0	35.0	40.0	30.0	41.0
22	36.338120717515984	38.0	35.0	40.0	30.0	41.0
23	36.25996087755562	38.0	35.0	40.0	30.0	41.0
24	36.28625759430247	38.0	34.0	40.0	30.0	41.0
25	36.2954021883424	38.0	35.0	40.0	31.0	41.0
26	35.946317572189784	38.0	34.0	40.0	30.0	41.0
27	35.52767968406223	37.0	34.0	40.0	29.0	41.0
28	35.44428564561278	37.0	34.0	40.0	27.0	41.0
29	35.425186645120974	37.0	34.0	40.0	27.0	41.0
30	35.22805833607585	37.0	34.0	40.0	27.0	41.0
31	35.27093771839975	37.0	34.0	40.0	27.0	41.0
32	34.61878542929302	37.0	33.0	40.0	24.0	41.0
33	34.15387730047738	37.0	33.0	40.0	22.0	41.0
34	33.719830789881414	37.0	33.0	40.0	18.0	41.0
35	33.09583149985394	37.0	33.0	40.0	12.0	41.0
36	33.01234131929166	37.0	32.0	40.0	13.0	41.0
37	32.76681839864218	36.0	32.0	40.0	12.0	41.0
38	32.775500770985786	36.0	31.0	40.0	12.0	41.0
39	32.65812234453635	36.0	31.0	40.0	11.0	41.0
40	32.56324302137683	36.0	31.0	40.0	12.0	41.0
41	32.61903318012225	36.0	31.0	40.0	14.0	41.0
42	32.47944962338176	36.0	31.0	40.0	12.0	41.0
43	32.20107938010524	36.0	31.0	40.0	11.0	41.0
44	32.17627101722053	35.0	30.0	40.0	11.0	41.0
45	32.19445481875363	35.0	30.0	40.0	12.0	41.0
46	32.03780603698513	35.0	30.0	40.0	11.0	41.0
47	31.999036729984876	35.0	30.0	40.0	11.0	41.0
48	32.14122721709259	35.0	30.0	40.0	12.0	41.0
49	32.04913416631846	35.0	30.0	40.0	11.0	41.0
50	31.788041770050253	35.0	30.0	39.0	10.0	40.0
51	31.6966919717638	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	13.0
15	20.0
16	85.0
17	218.0
18	514.0
19	1057.0
20	2174.0
21	4200.0
22	7233.0
23	10172.0
24	12936.0
25	15069.0
26	16085.0
27	15721.0
28	14630.0
29	14864.0
30	16694.0
31	20545.0
32	26557.0
33	40021.0
34	49407.0
35	49956.0
36	54775.0
37	81257.0
38	73160.0
39	13496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.233495912111316	21.79430764736552	26.247906135715688	16.724290304807475
2	28.217895005417237	32.79962134798638	27.952025085695904	11.030458560900481
3	25.60911575140608	30.57411632456098	32.063209741414695	11.753558182618246
4	22.604304947990812	24.756409166041127	39.671933528822294	12.967352357145762
5	27.735335554462658	32.25956151801	27.522713574157	12.482389353370335
6	18.349092011699756	44.91463689712425	27.92225800845311	8.814013082722893
7	32.158982076891505	53.19912880454678	13.867760221570594	0.7741288969911216
8	75.60560286651408	12.641208728224736	9.819252827872338	1.9339355773888542
9	71.85291735845847	5.329970824566529	10.628880351140578	12.18823146583442
10	56.74621810208074	19.431985001830398	14.76983208410216	9.051964811986704
11	52.55922908816601	17.076133460043707	19.330666005997788	11.033971445792488
12	39.45099155798294	18.141092248357264	23.654657530700764	18.753258662959034
13	26.917203151982193	36.273309840145245	24.04181442353559	12.76767258433697
14	14.200929620275632	46.58344210950587	26.121257390924924	13.094370879293576
15	12.69186822614104	20.102206461489537	48.261306867135296	18.944618445234124
16	13.249122703220392	16.01857021887122	45.14648729999667	25.585819777911716
17	13.660684901620733	23.807375579163786	29.29376222576387	33.23817729345161
18	18.327090258955085	21.753262360732602	33.56857336197876	26.35107401833356
19	27.675616511298546	20.989672118417502	25.180358905902757	26.154352464381198
20	36.59168814456816	20.17283393668672	25.243405945280344	17.99207197346478
21	20.470689597793168	33.48759212078408	26.177648437875554	19.8640698435472
22	19.60725946907367	22.37005099229754	31.573624520676102	26.449065017952687
23	25.092721672281122	28.607825228429963	24.903210776791294	21.396242322497624
24	19.531824888234794	27.061416321232983	36.80523456826645	16.601524222265773
25	15.811310010242833	30.511439062540447	34.70545384623918	18.97179708097754
26	15.202656480533072	30.99769628706556	34.6043197390851	19.195327493316274
27	23.66427174198415	31.42756246464004	27.69484493386532	17.21332085951049
28	14.162472775142088	32.84935640251005	37.615046980213215	15.37312384213465
29	15.994719579341279	22.032629153986385	41.96085536898234	20.011795897690003
30	17.067073914795902	35.150296006774326	30.889721298805988	16.892908779623788
31	28.588227028506136	22.605229391383446	23.609359804461732	25.19718377564868
32	36.10136336911546	24.1875067022146	24.53934985745083	15.171780071219118
33	33.221537312384214	24.106710349698446	23.729907222861115	18.941845115056225
34	20.817725647387707	23.568314517828814	27.101352275794742	28.512607558988734
35	23.084645734803075	24.22448443791993	30.030913387049658	22.65995644022734
36	30.121693728206246	27.876035838821444	23.782970273598266	18.21930015937404
37	19.50538580720548	32.1332825505763	28.23398032044906	20.12735132176916
38	20.95546771289007	31.12915213749801	22.951340997585355	24.964039152026565
39	21.36222280564872	31.011008271919476	24.049025081998128	23.577743840433673
40	22.442342465601463	24.1292667684787	25.461389697263275	27.96700106865656
41	16.089012805389874	24.02461977643261	24.850147726054143	35.036219692123375
42	21.118354638672056	24.664519492813376	23.945117644666148	30.272008223848424
43	21.795232090758155	26.34182958440723	24.773049147108527	27.08988917772609
44	19.993676807194387	28.43476942532901	28.57288126818842	22.99867249928818
45	18.339662689094897	34.068697237393366	22.78531096426841	24.806329109243325
46	23.608250472390573	32.75321428967619	23.28635928307566	20.35217595485758
47	21.037188508798852	27.352800878590998	28.301094910754237	23.308915701855913
48	22.876830860139112	22.651266672336586	30.322113055729144	24.149789411795158
49	21.18306567615639	22.58193341788909	32.678149486194364	23.556851419760164
50	19.28093095147412	32.0951954827998	26.14030092481317	22.483572640912904
51	19.49503204120799	31.764059859558557	24.410112671160693	24.330795428072758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	328.5
2	457.0
3	3604.0
4	6751.0
5	5225.0
6	3699.0
7	3793.0
8	3887.0
9	4032.0
10	4177.0
11	4111.5
12	4046.0
13	3891.0
14	3736.0
15	3545.0
16	3354.0
17	3192.5
18	3031.0
19	2895.0
20	2759.0
21	2676.5
22	2594.0
23	2488.5
24	2383.0
25	3120.5
26	4451.5
27	5045.0
28	6022.5
29	7000.0
30	7651.0
31	8302.0
32	9006.5
33	9711.0
34	9684.0
35	9657.0
36	11777.5
37	13898.0
38	20465.0
39	27032.0
40	34322.0
41	41612.0
42	43979.5
43	46347.0
44	44826.0
45	43305.0
46	42418.5
47	41532.0
48	43200.0
49	44868.0
50	39630.0
51	34392.0
52	32484.0
53	30576.0
54	29673.0
55	28770.0
56	29019.0
57	29268.0
58	26605.0
59	23942.0
60	20525.5
61	17109.0
62	14404.5
63	11700.0
64	9503.0
65	7306.0
66	6131.0
67	4956.0
68	4244.0
69	3532.0
70	2988.5
71	2445.0
72	2029.0
73	1613.0
74	1281.5
75	716.0
76	482.0
77	383.0
78	284.0
79	230.0
80	176.0
81	126.0
82	76.0
83	52.0
84	28.0
85	22.0
86	16.0
87	8.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.584882729378542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12214466126521	22.520647229123202
2	6.283795001038096	3.7181067640233443
3	2.8606261470199312	2.53893867276536
4	1.8843096938870898	2.229883252779229
5	1.3684873762572207	2.02432692716024
6	1.1417708340098223	2.0267493736803197
7	0.9128578524108812	1.8904754758517783
8	0.7573849830525632	1.7925716723681948
9	0.6996634876376605	1.8629516008609361
>10	7.522984152528661	46.66982014017659
>50	0.38742591393208065	7.225082710899584
>100	0.051075442029631206	2.5415308291350502
>500	0.002491484977055181	0.4717458615563899
>1k	0.004982969954110362	2.487169489619763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	2087	0.3858626720851376	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1909	0.3529524873073922	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1835	0.3392707250964194	No Hit
GAATCTATCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1791	0.33113562324124646	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	1667	0.3082094271039407	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1613	0.2982254384635011	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1390	0.2569952631520561	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	1205	0.2227908576246242	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	872	0.16122292767524674	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGGCGAGCCCCATCGTATGC	579	0.10705054486693563	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCAT	567	0.10483188072461572	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	542	0.10020966376144923	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010723543354546228	0.0
2	0.0	0.0	0.0	0.06027370919969086	0.0
3	0.0	0.0	0.0	0.07136702991129042	0.0
4	0.0	0.0	0.0	0.16473581256725325	0.0
5	0.0	0.0	0.0	0.48607233584658677	0.0
6	0.0	0.0	0.0	0.5628011374351503	0.0
7	0.0	0.0	0.0	0.6535814785917399	0.0
8	0.0	0.0	0.0	1.0525712468522703	0.0
9	0.0	0.0	0.0	1.2006670783521243	0.0
10	0.0	0.0	0.0	2.1036633842763273	0.0
11	0.0	0.0	0.0	2.2756098553061204	0.0
12	0.0	0.0	0.0	2.988910377061971	0.0
13	0.0	0.0	0.0	3.089859595537527	0.0
14	0.0	0.0	0.0	3.1432924236317312	0.0
15	0.0	0.0	0.0	3.273638941993026	0.0
16	0.0	0.0	0.0	3.377176601967955	0.0
17	0.0	0.0	0.0	3.4637045035184317	0.0
18	0.0	0.0	0.0	3.592571912451513	0.0
19	0.0	0.0	0.0	3.7815281419057585	0.0
20	0.0	0.0	0.0	3.858811609529902	0.0
21	0.0	0.0	0.0	3.930918194155299	0.0
22	0.0	0.0	0.0	4.011899435349976	0.0
23	0.0	0.0	0.0	4.0869642388318	0.0
24	0.0	0.0	0.0	4.158516157421617	0.0
25	0.0	0.0	0.0	4.2235969722630005	0.0
26	0.0	0.0	0.0	4.285349790890905	0.0
27	0.0	0.0	0.0	4.392215447079313	0.0
28	0.0	0.0	0.0	4.4460180525305715	0.0
29	0.0	0.0	0.0	4.500190435338882	0.0
30	0.0	0.0	0.0	4.576549459570392	0.0
31	0.0	0.0	0.0	4.644033827232623	0.0
32	0.0	0.0	0.0	4.722611515606453	0.0
33	0.0	0.0	0.0	4.779372339914138	0.0
34	0.0	0.0	0.0	4.860353581108814	0.0
35	0.0	0.0	0.0	4.949285035480138	0.0
36	0.0	0.0	0.0	5.021021842748481	0.0
37	0.0	0.0	0.0	5.105146191478111	0.0
38	0.0	0.0	0.0	5.1839087685304674	0.0
39	0.0	0.0	0.0	5.257679351262605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2103374E-7	45.000004	25
TTTAGTC	20	7.02997E-4	45.000004	33
GTGTTCG	20	7.02997E-4	45.000004	15
TTAGGTT	25	3.8880564E-5	45.000004	5
TGTCACG	20	7.02997E-4	45.000004	14
AAGTACG	25	3.8880564E-5	45.000004	1
AGCGTAA	30	2.1633623E-6	45.000004	1
TACCCTC	25	3.8880564E-5	45.000004	22
CTCACGT	30	2.1633623E-6	45.000004	45
AAATCGA	30	2.1633623E-6	45.000004	40
CAAACGA	80	0.0	45.000004	15
CGAACGT	20	7.02997E-4	45.000004	39
GTTTCGA	30	2.1633623E-6	45.000004	23
CGGCCTA	20	7.02997E-4	45.000004	28
TAGGTTA	25	3.8880564E-5	45.000004	6
CCGGCAT	25	3.8880564E-5	45.000004	18
CAGTTAT	20	7.02997E-4	45.000004	30
CCGGATT	20	7.02997E-4	45.000004	17
CCTACGT	20	7.02997E-4	45.000004	21
GTTAGAC	20	7.02997E-4	45.000004	9
>>END_MODULE