FastQCFastQC Report
Thu 26 May 2016
SRR1546431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546431_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1289690
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.32139506393009176No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40140.31123758422566666No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.1426699439400166No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.12879063961106932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA207.8568386E-444.00000438
ATCGTTC207.8568386E-444.00000435
GTTACAC207.8568386E-444.00000433
ACTACGC207.8568386E-444.00000416
TCGTAAT207.8568386E-444.00000424
GTATATC207.8568386E-444.00000423
ACGTAAT207.8568386E-444.00000436
CGGTCGA207.8568386E-444.00000438
AATAGTC207.8568386E-444.00000414
ATCGGCA207.8568386E-444.00000438
GGCGAAC207.8568386E-444.0000048
ACCCCGA207.8568386E-444.00000412
TATAACG207.8568386E-444.0000041
TAATGCG207.8568386E-444.0000041
CCCATCG207.8568386E-444.00000414
ATTTACG254.4431436E-544.01
ACTATCG302.5279587E-644.026
CGAGTAT254.4431436E-544.039
TCCGAAC502.7284841E-1144.036
TTTCGTA254.4431436E-544.01
Produced by FastQC (version 0.11.5)