##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546431_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1289690
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560541680558895	31.0	31.0	33.0	30.0	34.0
2	31.74903271328769	31.0	31.0	34.0	30.0	34.0
3	31.814839224930022	31.0	31.0	34.0	30.0	34.0
4	35.702580465073005	37.0	35.0	37.0	35.0	37.0
5	27.239025657328504	35.0	26.0	37.0	0.0	37.0
6	31.417267715497523	35.0	28.0	37.0	17.0	37.0
7	14.810732811760966	0.0	0.0	32.0	0.0	37.0
8	25.000117082399647	17.0	17.0	35.0	17.0	37.0
9	33.17793888453814	32.0	32.0	37.0	28.0	39.0
10	36.11062115702223	37.0	35.0	39.0	32.0	39.0
11	37.08745822639549	39.0	37.0	39.0	34.0	39.0
12	36.93236901891152	39.0	35.0	39.0	33.0	39.0
13	36.36797524986625	39.0	35.0	39.0	32.0	39.0
14	37.67651916351991	40.0	36.0	41.0	33.0	41.0
15	37.987368282300395	40.0	36.0	41.0	33.0	41.0
16	38.07583605362529	40.0	36.0	41.0	33.0	41.0
17	38.11470198264699	40.0	36.0	41.0	34.0	41.0
18	38.08035419364344	40.0	36.0	41.0	34.0	41.0
19	38.0107979436919	40.0	36.0	41.0	34.0	41.0
20	38.035097581589376	40.0	36.0	41.0	34.0	41.0
21	37.881597903372125	40.0	35.0	41.0	34.0	41.0
22	37.7933557676651	40.0	35.0	41.0	33.0	41.0
23	37.70663027549256	39.0	35.0	41.0	33.0	41.0
24	37.57640828416131	39.0	35.0	41.0	33.0	41.0
25	37.394535120842995	39.0	35.0	41.0	33.0	41.0
26	37.14227915235444	39.0	35.0	40.0	33.0	41.0
27	36.93869301925269	39.0	35.0	40.0	32.0	41.0
28	36.97322534872721	39.0	35.0	40.0	32.0	41.0
29	37.00769719855159	38.0	35.0	40.0	33.0	41.0
30	36.82038241748017	38.0	35.0	40.0	32.0	41.0
31	36.64083151765153	38.0	35.0	40.0	32.0	41.0
32	36.39589823911172	38.0	35.0	40.0	31.0	41.0
33	36.1972520528189	38.0	35.0	40.0	31.0	41.0
34	36.00747931673503	38.0	35.0	40.0	30.0	41.0
35	35.85709123897991	38.0	35.0	40.0	30.0	41.0
36	35.64945607083873	38.0	35.0	40.0	30.0	41.0
37	35.46072234412921	38.0	34.0	40.0	29.0	41.0
38	35.33043056858625	38.0	34.0	40.0	29.0	41.0
39	35.27711077855919	38.0	34.0	40.0	29.0	41.0
40	35.049689460257895	37.0	34.0	40.0	27.0	41.0
41	34.913504020345975	37.0	34.0	40.0	27.0	40.0
42	34.911867192891314	37.0	34.0	40.0	27.0	41.0
43	34.79120563856431	37.0	34.0	40.0	27.0	41.0
44	34.553262411897435	37.0	34.0	40.0	26.0	40.0
45	34.37335716334933	36.0	34.0	40.0	26.0	40.0
46	34.31759570129256	36.0	33.0	39.0	26.0	40.0
47	34.1958230272391	36.0	33.0	39.0	26.0	40.0
48	34.153266288798086	36.0	33.0	39.0	25.0	40.0
49	34.00130418937884	36.0	33.0	39.0	24.0	40.0
50	34.102909226248165	36.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	12.0
15	25.0
16	67.0
17	169.0
18	423.0
19	866.0
20	1569.0
21	2760.0
22	4361.0
23	6682.0
24	10300.0
25	14668.0
26	19088.0
27	20989.0
28	21917.0
29	25212.0
30	30891.0
31	42403.0
32	60334.0
33	117208.0
34	113864.0
35	134078.0
36	169432.0
37	319231.0
38	140613.0
39	32521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.829974645069747	23.965526599415362	29.040001860912312	15.164496894602578
2	30.41955818840186	25.04725941893013	30.54431685133637	13.988865541331638
3	29.014957082709795	23.44268777768301	33.85596538703099	13.6863897525762
4	26.667261124766416	27.006024703610947	31.701881847575773	14.624832324046864
5	17.721933177740386	48.26175282432212	23.541858896323923	10.474455101613566
6	21.23471531918523	40.25424714466267	27.868014794252883	10.643022741899216
7	38.72760120649148	56.63283424698958	3.6552194713458275	0.9843450751731037
8	87.8952306368197	3.388798858640448	7.1908753266288805	1.5250951779109707
9	83.4468748303856	5.4355697880886105	8.360613790910994	2.756941590614799
10	54.94110987911823	21.941396769766378	13.029720320387069	10.087773030728314
11	50.43506579100404	18.821344664221634	19.343330567810867	11.400258976963457
12	44.426955314843106	20.828571207034248	22.680566647799083	12.063906830323566
13	21.142600159728307	41.25363459435988	23.83363443928386	13.77013080662795
14	15.142010870829425	42.49726678504137	27.51754297544371	14.843179368685497
15	14.843024292659477	21.861377540339152	49.67558095356248	13.62001721343889
16	15.986632446556925	17.06650435375943	47.4212407632842	19.525622436399445
17	16.947871193852787	18.495685009575944	28.765439756840795	35.79100403973048
18	22.099341702269538	23.362125782164707	33.15889865006319	21.379633865502562
19	29.448161961401574	23.08430708154673	25.791314191782522	21.676216765269174
20	31.780195240716765	21.362110274562106	26.17140553156185	20.686288953159288
21	22.83370422349557	26.736192418333086	28.1026448216238	22.327458536547542
22	23.9935953601253	23.99708457071079	26.049128085043694	25.96019198412021
23	21.418713024060047	28.151881459885708	25.449604168443578	24.979801347610668
24	21.468880118478083	22.364056478688678	38.66634617621289	17.500717226620353
25	17.07953073994526	24.90179810652172	35.77022385224356	22.248447301289456
26	16.979506703161224	33.58225620110259	28.345028650295806	21.09320844544038
27	19.25974458978514	32.91891849979452	29.601144461072042	18.220192449348293
28	15.134334607541348	29.012320790267427	38.289899123045075	17.56344547914615
29	16.080841132365144	23.53619862137413	37.30338298350767	23.079577262753066
30	18.60912312261086	29.367599965883272	31.716924222099884	20.306352689405983
31	28.786375020353727	26.707503353519062	24.675076956477913	19.831044669649295
32	29.423427335251105	25.84225666633067	26.824275601113445	17.91004039730478
33	27.25259558498554	28.34595910645194	25.254983755786277	19.14646155277625
34	20.014964836511098	28.545309337902907	28.44063302033822	22.999092805247773
35	22.288922144081134	24.817436748365886	29.95401995828455	22.93962114926843
36	30.626274531088864	25.565756111933872	25.471159736060606	18.336809620916654
37	21.052345912583643	32.353898998984256	28.967348742721118	17.626406345710986
38	21.580302243174717	32.099729392334595	23.51503074382216	22.804937620668532
39	21.909683722444928	30.996906233280868	27.48334871170591	19.61006133256829
40	23.785715947243137	26.494196279726133	24.961424838527087	24.75866293450364
41	17.958269041397546	25.391062968620364	27.70960463367166	28.94106335631043
42	22.43841543316611	26.764338717056035	23.977312377393016	26.81993347238484
43	24.258542750583473	26.928874380665118	25.156898169327512	23.655684699423894
44	20.559979529964565	31.28558025572037	26.087354325458058	22.06708588885701
45	18.911676449379307	36.63469515930185	21.671254332436476	22.782374058882368
46	22.244880552690958	33.0498802037699	25.430374741216884	19.274864502322263
47	22.99188177003776	27.067900037993624	27.248253456256933	22.691964735711682
48	24.272034364847368	23.460986748753577	29.603703215501397	22.663275670897658
49	21.672107250579597	24.889624638479013	30.330932239530426	23.107335871410957
50	19.980693034760293	32.18013631182687	27.082709798478703	20.75646085493413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	1093.5
2	1748.0
3	7573.5
4	13399.0
5	10072.5
6	6746.0
7	6966.0
8	7186.0
9	7451.5
10	7717.0
11	7731.5
12	7746.0
13	7236.5
14	6727.0
15	6227.5
16	5728.0
17	5468.5
18	5209.0
19	5106.5
20	5004.0
21	5036.0
22	5068.0
23	6028.5
24	6989.0
25	9325.0
26	11661.0
27	13204.5
28	14748.0
29	17615.5
30	20483.0
31	22610.0
32	24737.0
33	26607.0
34	28477.0
35	29655.0
36	30833.0
37	37172.5
38	43512.0
39	60631.0
40	77750.0
41	99054.5
42	120359.0
43	125380.0
44	130401.0
45	124224.0
46	118047.0
47	109934.5
48	101822.0
49	97582.5
50	93343.0
51	87670.5
52	81998.0
53	73204.0
54	64410.0
55	58731.0
56	53052.0
57	50730.0
58	48408.0
59	46710.0
60	45012.0
61	40030.5
62	35049.0
63	29330.0
64	23611.0
65	19840.5
66	16070.0
67	13065.0
68	10060.0
69	8523.0
70	6986.0
71	5497.5
72	4009.0
73	3227.5
74	2446.0
75	1859.0
76	1272.0
77	1030.0
78	788.0
79	591.0
80	394.0
81	260.5
82	127.0
83	96.0
84	65.0
85	46.5
86	28.0
87	23.0
88	18.0
89	11.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1289690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.098490115263118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19559790699492	12.686326974087894
2	5.60681799139247	1.917362440078071
3	2.1430045404878526	1.0992642585748658
4	1.2800095928775224	0.8754492548503313
5	0.9869956360673353	0.8438067563552584
6	0.8291128149546485	0.8505946362564023
7	0.7080022121568963	0.8474038178304376
8	0.6774047890864066	0.9266079272180656
9	0.583041974996443	0.8972223701634148
>10	10.279824127306215	42.39448791827547
>50	2.404389686195723	28.270061251182398
>100	0.29966387646923576	6.803599276244772
>500	0.0028314696989219126	0.3721411781830794
>1k	0.003303381315408898	1.215671940699539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4145	0.32139506393009176	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4014	0.31123758422566666	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1840	0.1426699439400166	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.12879063961106932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0065907311059246795	0.0
2	0.0	0.0	0.0	0.023494017942296212	0.0
3	0.0	0.0	0.0	0.033573959633710425	0.0
4	0.0	0.0	0.0	0.05001201839201669	0.0
5	0.0	0.0	0.0	0.08009676744023758	0.0
6	0.0	0.0	0.0	0.12716234133784088	0.0
7	0.0	0.0	0.0	0.16228706123176886	0.0
8	0.0	0.0	0.0	0.35822562011025905	0.0
9	0.0	0.0	0.0	0.43669408927726816	0.0
10	0.0	0.0	0.0	0.5826206297637416	0.0
11	0.0	0.0	0.0	0.7243601175476277	0.0
12	0.0	0.0	0.0	0.8417526692460979	0.0
13	0.0	0.0	0.0	0.8892059332087556	0.0
14	0.0	0.0	0.0	0.9062642960711489	0.0
15	0.0	0.0	0.0	0.934255518768076	0.0
16	0.0	0.0	0.0	1.0011708239964643	0.0
17	0.0	0.0	0.0	1.08111251541068	0.0
18	0.0	0.0	0.0	1.1905186517690298	0.0
19	0.0	0.0	0.0	1.2404531321480357	0.0
20	0.0	0.0	0.0	1.2938768231125308	0.0
21	0.0	0.0	0.0	1.3665299413037242	0.0
22	0.0	0.0	0.0	1.43677938109158	0.0
23	0.0	0.0	0.0	1.5208305871953725	0.0
24	0.0	0.0	0.0	1.5916227930743048	0.0
25	0.0	0.0	0.0	1.6465972442990175	0.0
26	0.0	0.0	0.0	1.7014166194977087	0.0
27	0.0	0.0	0.0	1.7584070590607046	0.0
28	0.0	0.0	0.0	1.8198947033783313	0.0
29	0.0	0.0	0.0	1.8799866634617621	0.0
30	0.0	0.0	0.0	1.9593855887849019	0.0
31	0.0	0.0	0.0	2.037621443912878	0.0
32	0.0	0.0	0.0	2.104149059076212	0.0
33	0.0	0.0	0.0	2.1728477386038505	0.0
34	0.0	0.0	0.0	2.2461986989121416	0.0
35	0.0	0.0	0.0	2.3420356829935876	0.0
36	0.0	0.0	0.0	2.4248462808892057	0.0
37	0.0	0.0	0.0	2.501376299730943	0.0
38	0.0	0.0	0.0	2.579844768897952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAA	20	7.8568386E-4	44.000004	38
ATCGTTC	20	7.8568386E-4	44.000004	35
GTTACAC	20	7.8568386E-4	44.000004	33
ACTACGC	20	7.8568386E-4	44.000004	16
TCGTAAT	20	7.8568386E-4	44.000004	24
GTATATC	20	7.8568386E-4	44.000004	23
ACGTAAT	20	7.8568386E-4	44.000004	36
CGGTCGA	20	7.8568386E-4	44.000004	38
AATAGTC	20	7.8568386E-4	44.000004	14
ATCGGCA	20	7.8568386E-4	44.000004	38
GGCGAAC	20	7.8568386E-4	44.000004	8
ACCCCGA	20	7.8568386E-4	44.000004	12
TATAACG	20	7.8568386E-4	44.000004	1
TAATGCG	20	7.8568386E-4	44.000004	1
CCCATCG	20	7.8568386E-4	44.000004	14
ATTTACG	25	4.4431436E-5	44.0	1
ACTATCG	30	2.5279587E-6	44.0	26
CGAGTAT	25	4.4431436E-5	44.0	39
TCCGAAC	50	2.7284841E-11	44.0	36
TTTCGTA	25	4.4431436E-5	44.0	1
>>END_MODULE