FastQCFastQC Report
Thu 26 May 2016
SRR1546430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546430_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences489895
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15850.3235387174802764No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.23066167239918756No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.199022239459476No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5480.11186070484491574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG207.029317E-445.00000419
CAACGTC207.029317E-445.00000423
TTAGCGA207.029317E-445.0000041
TTCGTGC207.029317E-445.00000431
GTTAGCG207.029317E-445.00000424
CCATCGC207.029317E-445.00000410
CATAGTA207.029317E-445.0000041
CGCTAAG207.029317E-445.0000042
TACCTAC207.029317E-445.00000443
CGCGTAT207.029317E-445.00000414
ATACCTA207.029317E-445.00000442
ATCGCGT207.029317E-445.00000412
ATCGCGA207.029317E-445.0000041
TCGTTAG253.887518E-545.02
CGAACGT253.887518E-545.038
CTCGTAG253.887518E-545.01
ATCTACG253.887518E-545.023
ATAGGCC253.887518E-545.05
CTATCTC502.1827873E-1145.05
TTTGTCA453.8380676E-1045.040