##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546430_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	489895
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.043721613815205	31.0	31.0	33.0	30.0	33.0
2	31.197666846977413	31.0	31.0	33.0	30.0	34.0
3	31.160971228528563	31.0	31.0	34.0	28.0	34.0
4	35.13380622378265	35.0	35.0	37.0	33.0	37.0
5	32.86061707100501	35.0	35.0	37.0	28.0	37.0
6	34.03693852764368	35.0	35.0	37.0	28.0	37.0
7	14.914877677869747	0.0	0.0	35.0	0.0	37.0
8	24.70913154859715	17.0	17.0	35.0	17.0	37.0
9	32.6295573541269	32.0	32.0	37.0	27.0	39.0
10	35.540879167984976	37.0	35.0	37.0	32.0	39.0
11	36.52480429479787	37.0	35.0	39.0	32.0	39.0
12	36.268469774135276	37.0	35.0	39.0	32.0	39.0
13	35.819975709080516	37.0	35.0	39.0	31.0	39.0
14	36.857210218516215	39.0	35.0	40.0	31.0	41.0
15	37.267992120760574	39.0	35.0	41.0	32.0	41.0
16	37.360954898498655	39.0	35.0	41.0	32.0	41.0
17	37.34704579552761	39.0	35.0	41.0	32.0	41.0
18	37.241849784137415	39.0	35.0	40.0	32.0	41.0
19	37.16478429051123	39.0	35.0	40.0	32.0	41.0
20	37.27546515069556	39.0	35.0	40.0	32.0	41.0
21	37.12158115514549	39.0	35.0	40.0	32.0	41.0
22	37.15621306606518	39.0	35.0	40.0	32.0	41.0
23	37.15614570469182	39.0	35.0	40.0	32.0	41.0
24	36.999465191520635	39.0	35.0	40.0	32.0	41.0
25	36.73771114218353	38.0	35.0	40.0	31.0	41.0
26	36.594894824401145	38.0	35.0	40.0	31.0	41.0
27	36.513332448790045	38.0	35.0	40.0	31.0	41.0
28	36.43939211463681	38.0	35.0	40.0	31.0	41.0
29	36.54139968768818	38.0	35.0	40.0	31.0	41.0
30	36.28665734494126	38.0	35.0	40.0	30.0	41.0
31	36.16385756131416	38.0	35.0	40.0	30.0	41.0
32	36.055644576899134	38.0	35.0	40.0	30.0	41.0
33	35.84641402749569	38.0	34.0	40.0	30.0	41.0
34	35.70585533634758	38.0	34.0	40.0	29.0	41.0
35	35.53230998479266	38.0	34.0	40.0	28.0	41.0
36	35.3016768899458	38.0	34.0	40.0	27.0	41.0
37	35.13350820073689	38.0	34.0	40.0	26.0	41.0
38	35.128237683585255	38.0	34.0	40.0	27.0	41.0
39	35.03723859194317	38.0	34.0	40.0	26.0	41.0
40	34.85178864858796	38.0	34.0	40.0	24.0	41.0
41	34.76088549587156	37.0	34.0	40.0	24.0	41.0
42	34.726790434684986	37.0	34.0	40.0	24.0	41.0
43	34.6510823747946	37.0	33.0	40.0	24.0	41.0
44	34.411665765112936	37.0	33.0	40.0	24.0	41.0
45	34.33768052337746	37.0	33.0	40.0	23.0	41.0
46	34.32442870411006	37.0	33.0	40.0	23.0	41.0
47	34.30336296553343	37.0	33.0	40.0	23.0	41.0
48	34.269827207871074	36.0	33.0	40.0	23.0	41.0
49	34.17223690790884	36.0	33.0	40.0	24.0	41.0
50	34.04775104869411	36.0	33.0	40.0	24.0	41.0
51	33.86437910164423	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	5.0
15	13.0
16	57.0
17	139.0
18	289.0
19	537.0
20	942.0
21	1523.0
22	2392.0
23	3534.0
24	5038.0
25	6736.0
26	8063.0
27	8802.0
28	9808.0
29	11256.0
30	14117.0
31	18664.0
32	24716.0
33	38883.0
34	49138.0
35	49838.0
36	56348.0
37	87448.0
38	77275.0
39	14331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.38427622245583	24.694271221384174	29.23748966615295	14.683962890007043
2	29.97234101184948	24.981883873074842	30.963777952418376	14.081997162657304
3	29.440186162340908	23.167413425325837	33.584951877443125	13.80744853489013
4	27.16643362353157	27.6834831953786	30.41610957450066	14.733973606589167
5	21.491544106390144	37.09509180538687	28.492636177140003	12.920727911082988
6	21.232304881658315	40.92754569856806	27.411588197470888	10.428561222302738
7	37.279008767184806	58.186346053746206	3.8422519111238125	0.692393267945172
8	88.39567662458282	3.657110197083049	6.518335561701997	1.4288776166321353
9	83.72304269282193	5.72163422774268	7.67225629981935	2.88306677961604
10	55.14508210943161	22.398677267577746	12.487165617122036	9.969075005868605
11	51.06584063932067	18.959164718970392	18.754018718296777	11.22097592341216
12	44.811235060574205	21.23781626675104	21.977974872166484	11.972973800508273
13	21.22556874432276	42.107798609906204	23.04105981894079	13.62557282683024
14	14.907888425070679	43.45012706804519	26.8963757539881	14.745608752896027
15	14.779697690321395	22.224966574470038	49.42140662795089	13.57392910725768
16	15.838496004245808	17.36555792567795	47.221139223711205	19.574806846365036
17	16.738076526602637	18.850774145480152	28.44854509639821	35.962604231518995
18	21.981240878147357	23.68915788077037	33.044223762234765	21.285377478847508
19	30.018269220955514	23.10576756243685	25.30950509803121	21.566458118576428
20	32.25609569397524	21.614631706794313	25.629369558783004	20.499903040447442
21	22.8057032629441	27.389134406352383	27.530593290398965	22.274569040304552
22	23.867767582849385	24.376243888996623	25.327468130926018	26.428520397227977
23	21.323140673001355	28.544484022086365	25.193153634962595	24.93922166994968
24	20.98449667785954	22.687718796885047	38.976719501117586	17.351065024137828
25	16.991396115494137	25.306239092050337	35.81951234448198	21.882852447973544
26	16.657651129323632	34.43472580859164	27.90475510058278	21.002867961501952
27	18.9861092683126	34.10281795078537	28.91701282927974	17.994059951622287
28	14.874207738392922	29.74576184692638	38.09020300268425	17.28982741199645
29	15.994856040580125	24.016983231100543	37.094071178517844	22.89408954980149
30	18.486206227865154	30.38140826095388	31.060737504975556	20.07164800620541
31	29.2574939527858	27.062125557517426	24.219067351167087	19.461313138529686
32	30.218924463405422	25.801038998152663	26.49261576460262	17.487420773839293
33	27.936598658896294	28.44854509639821	24.629155227140508	18.98570101756499
34	20.102062686902293	28.665734494126294	27.922309882729973	23.30989293624144
35	22.377448228702068	24.878392308555917	29.960705865542614	22.783453597199397
36	31.15014441870197	26.108655936476183	24.89186458322702	17.849335061594832
37	20.95775625389114	33.322650772104225	28.489166045785318	17.230426928219313
38	21.35743373580053	33.04238663387052	23.1657804223354	22.43439920799355
39	21.969401606466693	31.715571704140682	27.195011175864213	19.12001551352841
40	24.106186019453148	26.696945263781014	24.599965298686453	24.596903418079386
41	17.813000745057614	25.602629134814602	27.45180089611039	29.13256922401739
42	22.195776646015982	26.59733208136437	23.781422549730046	27.4254687228896
43	24.03412976250013	26.688167872707417	25.25867787995387	24.01902448483859
44	20.23025342165158	31.25710611457557	26.47424448096021	22.038395982812645
45	18.831382234968718	37.591932965227244	21.329264434215496	22.247420365588543
46	22.018799946927402	33.93380214127517	24.893497586217457	19.15390032557997
47	22.857142857142858	27.30360587472826	26.9986425662642	22.840608701864685
48	23.99963257432715	23.79999795874626	29.928454056481492	22.271915410445096
49	21.729350166872493	25.31705773686198	30.49878035089152	22.45481174537401
50	19.917941599730554	33.197521917962014	26.671633717429245	20.212902764878187
51	19.382520744241113	33.782136988538355	23.196195103032284	23.639147164188245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	437.0
2	681.0
3	2418.0
4	4155.0
5	3182.5
6	2210.0
7	2294.0
8	2378.0
9	2478.5
10	2579.0
11	2564.5
12	2550.0
13	2409.0
14	2268.0
15	2115.0
16	1962.0
17	1894.0
18	1826.0
19	1830.0
20	1834.0
21	1859.5
22	1885.0
23	2071.5
24	2258.0
25	3001.5
26	4461.0
27	5177.0
28	6255.5
29	7334.0
30	8232.5
31	9131.0
32	10014.5
33	10898.0
34	11613.0
35	12328.0
36	13639.0
37	14950.0
38	20048.0
39	25146.0
40	33408.0
41	41670.0
42	45599.5
43	49529.0
44	46925.5
45	44322.0
46	42122.0
47	39922.0
48	37495.0
49	35068.0
50	33742.5
51	32417.0
52	29741.5
53	27066.0
54	24548.5
55	22031.0
56	20741.5
57	19452.0
58	18493.0
59	17534.0
60	16058.5
61	14583.0
62	12645.5
63	10708.0
64	8834.0
65	6960.0
66	5856.0
67	4752.0
68	3958.5
69	3165.0
70	2710.0
71	2255.0
72	1787.0
73	1319.0
74	1005.0
75	560.0
76	429.0
77	345.5
78	262.0
79	211.0
80	160.0
81	109.0
82	58.0
83	42.0
84	26.0
85	20.0
86	14.0
87	12.5
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489895.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.460014453462403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5520420033528	17.012439526698337
2	6.064532128999982	2.966770870576534
3	3.215968864681979	2.3598793473601885
4	2.2942895429614154	2.244734215250555
5	1.8648908636918715	2.2807628740016583
6	1.4657605732618637	2.1511514884380314
7	1.2552603506964792	2.1492580424625913
8	1.0883068286121196	2.1296000606123417
9	1.0223674482746405	2.2506410305392466
>10	11.941228701635582	59.11651796405919
>50	0.2086460051695555	3.2872563086715627
>100	0.023368352578990215	1.185845612437006
>500	0.0016691680413564443	0.3109042636842073
>1k	0.0016691680413564443	0.5542383952085508
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1585	0.3235387174802764	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1130	0.23066167239918756	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	975	0.199022239459476	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	548	0.11186070484491574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0034701313546780434	0.0
2	0.0	0.0	0.0	0.01857540901621776	0.0
3	0.0	0.0	0.0	0.024903295604160075	0.0
4	0.0	0.0	0.0	0.03735494340624011	0.0
5	0.0	0.0	0.0	0.07205625695302055	0.0
6	0.0	0.0	0.0	0.10614519437838721	0.0
7	0.0	0.0	0.0	0.12717010788026006	0.0
8	0.0	0.0	0.0	0.27761050837424345	0.0
9	0.0	0.0	0.0	0.33741924289898856	0.0
10	0.0	0.0	0.0	0.46009859255554764	0.0
11	0.0	0.0	0.0	0.5642025331958889	0.0
12	0.0	0.0	0.0	0.6550383245389318	0.0
13	0.0	0.0	0.0	0.6895355127119076	0.0
14	0.0	0.0	0.0	0.7036201635044245	0.0
15	0.0	0.0	0.0	0.7234203247634697	0.0
16	0.0	0.0	0.0	0.7758805458312495	0.0
17	0.0	0.0	0.0	0.8434460445605692	0.0
18	0.0	0.0	0.0	0.9348942120250258	0.0
19	0.0	0.0	0.0	0.9751069106645301	0.0
20	0.0	0.0	0.0	1.0247093764990458	0.0
21	0.0	0.0	0.0	1.089417119995101	0.0
22	0.0	0.0	0.0	1.1508588575102827	0.0
23	0.0	0.0	0.0	1.2257728696965677	0.0
24	0.0	0.0	0.0	1.2827238489880484	0.0
25	0.0	0.0	0.0	1.3411037058961615	0.0
26	0.0	0.0	0.0	1.3886649179926311	0.0
27	0.0	0.0	0.0	1.4388797599485603	0.0
28	0.0	0.0	0.0	1.4848079690545932	0.0
29	0.0	0.0	0.0	1.53624756325335	0.0
30	0.0	0.0	0.0	1.6044254381040834	0.0
31	0.0	0.0	0.0	1.6666836771144837	0.0
32	0.0	0.0	0.0	1.72343053103216	0.0
33	0.0	0.0	0.0	1.782218638687882	0.0
34	0.0	0.0	0.0	1.8491717612957879	0.0
35	0.0	0.0	0.0	1.9295971585747966	0.0
36	0.0	0.0	0.0	2.011247308096633	0.0
37	0.0	0.0	0.0	2.0733014217332286	0.0
38	0.0	0.0	0.0	2.1431122995743985	0.0
39	0.0	0.0	0.0	2.2119025505465455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGG	20	7.029317E-4	45.000004	19
CAACGTC	20	7.029317E-4	45.000004	23
TTAGCGA	20	7.029317E-4	45.000004	1
TTCGTGC	20	7.029317E-4	45.000004	31
GTTAGCG	20	7.029317E-4	45.000004	24
CCATCGC	20	7.029317E-4	45.000004	10
CATAGTA	20	7.029317E-4	45.000004	1
CGCTAAG	20	7.029317E-4	45.000004	2
TACCTAC	20	7.029317E-4	45.000004	43
CGCGTAT	20	7.029317E-4	45.000004	14
ATACCTA	20	7.029317E-4	45.000004	42
ATCGCGT	20	7.029317E-4	45.000004	12
ATCGCGA	20	7.029317E-4	45.000004	1
TCGTTAG	25	3.887518E-5	45.0	2
CGAACGT	25	3.887518E-5	45.0	38
CTCGTAG	25	3.887518E-5	45.0	1
ATCTACG	25	3.887518E-5	45.0	23
ATAGGCC	25	3.887518E-5	45.0	5
CTATCTC	50	2.1827873E-11	45.0	5
TTTGTCA	45	3.8380676E-10	45.0	40
>>END_MODULE