FastQCFastQC Report
Thu 26 May 2016
SRR1546429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546429_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1003897
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39040.38888451703710636No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.3548172770712533No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.1549959806633549No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.14134916231446054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA302.5273948E-644.0000041
CGGCCTC302.5273948E-644.00000419
GGTCCGT302.5273948E-644.0000049
CCCGTGC302.5273948E-644.00000439
AACGACC302.5273948E-644.00000427
GTCCAAC302.5273948E-644.00000414
ATACCGG302.5273948E-644.00000415
CGGGTAC254.4424356E-544.06
AGCGTAA351.4458419E-744.01
GTACCGA254.4424356E-544.027
ACGCCGG700.044.027
AATCATA408.310963E-944.032
ATCTACG254.4424356E-544.01
GTCGACA207.856005E-444.031
GGTCGAC254.4424356E-544.09
CGATTCT502.7284841E-1144.010
TCGATCT351.4458419E-744.016
ACGGGTA551.8189894E-1244.05
CGTTCAC254.4424356E-544.026
GCGCAAA207.856005E-444.01
Produced by FastQC (version 0.11.5)