FastQCFastQC Report
Thu 26 May 2016
SRR1546428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546428_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences386141
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14550.3768053638437773No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.25664200382761737No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.22401143623702224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA253.885977E-545.0000041
TTAGGAC650.045.0000045
TCACCGG253.885977E-545.00000417
CATGATA253.885977E-545.0000041
CGGTTTA253.885977E-545.0000041
TTGTAGC253.885977E-545.00000434
ATCACGG253.885977E-545.0000042
ATCGTCC253.885977E-545.00000419
TTGCGAA253.885977E-545.0000041
GCGATAA650.045.0000049
TTCATAG253.885977E-545.0000042
ATTGCGA253.885977E-545.0000041
TTACCGG253.885977E-545.0000042
CCGCAAT502.1827873E-1145.00000433
GCGTAAA253.885977E-545.00000421
CTTGTAT207.0274604E-445.018
GTGCATC207.0274604E-445.015
CGGGTTC207.0274604E-445.06
CTTCGCA406.7939254E-945.024
AACGTCA207.0274604E-445.034