##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546428_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	386141
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114507394967124	31.0	31.0	33.0	30.0	33.0
2	31.259078937486567	31.0	31.0	33.0	30.0	34.0
3	31.202180032682364	31.0	31.0	34.0	28.0	34.0
4	35.16670853392932	35.0	35.0	37.0	33.0	37.0
5	32.92866336390075	35.0	35.0	37.0	28.0	37.0
6	34.10762648876965	35.0	35.0	37.0	29.0	37.0
7	15.203060540061792	0.0	0.0	35.0	0.0	37.0
8	24.887152620415858	17.0	17.0	35.0	17.0	37.0
9	32.81725587285474	32.0	32.0	37.0	27.0	39.0
10	35.699262704556105	37.0	35.0	37.0	32.0	39.0
11	36.62700930489122	37.0	35.0	39.0	33.0	39.0
12	36.2839972963244	37.0	35.0	39.0	32.0	39.0
13	35.707759600767595	37.0	35.0	39.0	31.0	39.0
14	36.695823546321165	39.0	35.0	40.0	31.0	41.0
15	37.18968718680482	39.0	35.0	41.0	32.0	41.0
16	37.346798190298365	39.0	35.0	41.0	32.0	41.0
17	37.351141163461016	39.0	35.0	41.0	32.0	41.0
18	37.24293457571198	39.0	35.0	41.0	32.0	41.0
19	37.14742283259224	39.0	35.0	41.0	32.0	41.0
20	37.2300066555999	39.0	35.0	41.0	32.0	41.0
21	37.04929287488249	39.0	35.0	40.0	32.0	41.0
22	37.08284797522148	39.0	35.0	41.0	32.0	41.0
23	37.10689618559024	39.0	35.0	41.0	32.0	41.0
24	36.93116763047695	38.0	35.0	41.0	32.0	41.0
25	36.692083979686174	38.0	35.0	40.0	31.0	41.0
26	36.55737929927151	38.0	35.0	40.0	31.0	41.0
27	36.46300185683468	38.0	35.0	40.0	31.0	41.0
28	36.41571343110418	38.0	35.0	40.0	31.0	41.0
29	36.510487101861756	38.0	35.0	40.0	31.0	41.0
30	36.2970365747227	38.0	35.0	40.0	31.0	41.0
31	36.13741612519779	38.0	35.0	40.0	30.0	41.0
32	35.98774540906042	38.0	34.0	40.0	30.0	41.0
33	35.79467085857239	38.0	34.0	40.0	30.0	41.0
34	35.651114489266874	38.0	34.0	40.0	29.0	41.0
35	35.47527457586737	38.0	34.0	40.0	29.0	41.0
36	35.24321167656374	38.0	34.0	40.0	27.0	41.0
37	35.038050867429256	38.0	34.0	40.0	26.0	41.0
38	35.02110886955801	38.0	34.0	40.0	27.0	41.0
39	34.933892023898004	37.0	34.0	40.0	26.0	41.0
40	34.740146733965055	37.0	34.0	40.0	24.0	41.0
41	34.677130892601404	37.0	34.0	40.0	24.0	41.0
42	34.64643744124555	37.0	34.0	40.0	24.0	41.0
43	34.55715917242665	37.0	33.0	40.0	24.0	41.0
44	34.195239562750395	36.0	33.0	40.0	23.0	41.0
45	34.135854519463095	36.0	33.0	40.0	23.0	41.0
46	34.18577669814912	36.0	33.0	40.0	23.0	41.0
47	34.18141041743819	36.0	33.0	40.0	23.0	41.0
48	34.1934474712605	36.0	33.0	40.0	23.0	41.0
49	34.13356779000417	36.0	33.0	40.0	24.0	41.0
50	34.000248613848306	36.0	33.0	40.0	24.0	41.0
51	33.79217177145136	35.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	12.0
16	33.0
17	105.0
18	221.0
19	397.0
20	680.0
21	1175.0
22	1773.0
23	2732.0
24	4113.0
25	5464.0
26	6664.0
27	7018.0
28	7757.0
29	8832.0
30	11173.0
31	14523.0
32	20018.0
33	31737.0
34	40621.0
35	39683.0
36	41958.0
37	64625.0
38	63370.0
39	11454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.45022932037779	24.438482316045178	30.919534574158142	16.191753789418893
2	30.7695893468966	25.55361901481583	30.87654509622133	12.80024654206624
3	28.219225619657067	23.62660271766013	34.410487360834516	13.743684301848289
4	27.672275153376617	26.87256727464838	30.836663291388376	14.618494280586624
5	21.1280335421517	36.37168806213275	28.81123734594358	13.689041049771975
6	21.260109649066013	39.51509940669341	28.589815637293114	10.634975306947462
7	37.282754227082854	57.6478540222354	4.319665614374024	0.7497261363077218
8	88.34337716015652	3.420512196322069	6.824450136090185	1.4116605074312232
9	84.375914497554	4.759660331329747	8.256828464213848	2.607596706902401
10	59.467655597307726	19.076451348082696	12.389256774079934	9.066636280529652
11	56.074076567885825	16.25934567942798	17.770969671700236	9.89560808098596
12	48.96190769692936	20.276272138933706	20.206867439614026	10.554952724522908
13	20.373904868946834	46.528858629360776	20.88900168591266	12.20823481577973
14	13.346938035588035	48.07803367163808	26.032718618328538	12.542309674445345
15	11.601979587767163	21.836065064315886	54.51324775146902	12.04870759644793
16	13.36454818317661	15.12141937789564	52.55955726017181	18.95447517875595
17	14.369880432277329	16.747250356734973	29.299142023250575	39.583727187737125
18	21.15703849112112	22.441543374052483	34.924030341248404	21.477387793577993
19	31.62238664114922	21.375611499426377	26.060169730746026	20.941832128678385
20	34.05155111733797	20.91489896177821	24.40947736707576	20.624072553808066
21	22.119640235043676	28.180897651376053	27.531393972668017	22.168068140912258
22	22.47132524129787	24.790426295058023	24.938299740250322	27.799948723393786
23	20.312787297904134	30.76440989172349	24.20592477877252	24.716878031599858
24	19.82306981128655	21.720563213955522	41.8805565842529	16.575810390505023
25	15.6875338283166	23.94254948321986	39.217539706998224	21.15237698146532
26	15.28146454274475	36.464400309731424	28.445826783480644	19.808308364043185
27	17.070966305054373	35.73875863997866	29.75752380607084	17.43275124889613
28	13.42644267249528	28.984490121484118	41.73915745802699	15.84990974799361
29	13.93998565290917	23.842585998378834	40.7843248968641	21.433103451847902
30	16.936818416070814	30.619903092393713	32.94651435615488	19.496764135380598
31	30.95915740623244	26.04644417453728	24.455315545357784	18.5390828738725
32	32.57980892989866	25.311997430990235	26.198461183868073	15.909732455243034
33	29.561999373285925	26.99972289914824	25.154283020969025	18.28399470659681
34	19.271716808108955	26.715111837385823	28.956780036307983	25.056391318197242
35	20.558034500350907	24.3550930877581	32.18332163639707	22.903550775493926
36	32.143439831564116	23.67166397766619	26.04489033798535	18.14000585278435
37	21.25751992147946	32.771189798545095	29.169137698405507	16.802152581569946
38	20.410937973434574	34.3418595797908	23.17236449897835	22.074837947796272
39	20.813899585902558	32.860017454763934	26.273822256636826	20.052260702696685
40	24.116579177036368	26.04540828350266	24.685801300561195	25.15221123889978
41	16.663084210171935	23.853462854242363	27.139309216063563	32.344143719522144
42	21.777019275342425	25.56708559826592	23.26844339243955	29.38745173395211
43	23.40984251866546	26.718996428765657	24.242180964984293	25.628980087584587
44	20.098357853737365	31.57318181700467	27.55832713956819	20.770133189689776
45	16.021090741464906	39.964158170202076	21.47169039288757	22.543060695445448
46	21.316306737694262	34.8885510733126	24.74044455263751	19.054697636355634
47	22.757490139612212	26.411336791482903	27.60520120888484	23.225971860020046
48	24.32039073809826	21.643907277393488	31.623681504942496	22.412020479565754
49	19.908271848884215	22.9258224327383	33.93190570283912	23.234000015538363
50	18.12472646002367	34.815779728130394	26.206230366627736	20.853263445218197
51	18.47433968420862	35.57793655685359	22.372656620250115	23.57506713868768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	288.5
2	431.0
3	1932.0
4	3433.0
5	2631.5
6	1830.0
7	1952.0
8	2074.0
9	2156.0
10	2238.0
11	2232.0
12	2226.0
13	2091.0
14	1956.0
15	1870.0
16	1784.0
17	1678.0
18	1572.0
19	1527.0
20	1482.0
21	1533.5
22	1585.0
23	1630.5
24	1676.0
25	1954.0
26	2755.5
27	3279.0
28	3953.5
29	4628.0
30	5447.0
31	6266.0
32	6666.0
33	7066.0
34	7769.5
35	8473.0
36	9840.5
37	11208.0
38	17019.0
39	22830.0
40	30514.0
41	38198.0
42	40935.5
43	43673.0
44	40491.5
45	37310.0
46	35293.0
47	33276.0
48	30896.0
49	28516.0
50	26729.5
51	24943.0
52	22740.0
53	20537.0
54	18042.5
55	15548.0
56	14341.5
57	13135.0
58	12601.5
59	12068.0
60	10911.0
61	9754.0
62	8366.5
63	6979.0
64	5886.5
65	4794.0
66	4088.0
67	3382.0
68	2817.0
69	2252.0
70	1874.5
71	1497.0
72	1153.5
73	810.0
74	649.5
75	402.0
76	315.0
77	213.0
78	111.0
79	89.0
80	67.0
81	57.5
82	48.0
83	31.5
84	15.0
85	11.5
86	8.0
87	4.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	386141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.944515086458054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52689444669386	17.343146674401318
2	6.103549589393798	3.0450016962715694
3	3.2568183469856002	2.4371926317070707
4	2.29960237123784	2.294498641687881
5	1.7846575513127978	2.225870860644169
6	1.4046781075777868	2.102340854765487
7	1.2624453649775231	2.204376121675761
8	1.0745320335129411	2.144294441667681
9	1.0807612047217119	2.426315775843539
>10	11.973505258458696	58.89299504585112
>50	0.2107536258967411	3.276782315268257
>100	0.01868751362631202	0.7497261363077218
>500	0.002076390402923558	0.48065344006463956
>1k	0.001038195201461779	0.3768053638437773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1455	0.3768053638437773	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.25664200382761737	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.22401143623702224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0036256186211772383	0.0
2	0.0	0.0	0.0	0.021235766209752396	0.0
3	0.0	0.0	0.0	0.03185364931462859	0.0
4	0.0	0.0	0.0	0.050758660696481335	0.0
5	0.0	0.0	0.0	0.0883097107015313	0.0
6	0.0	0.0	0.0	0.12974535208641402	0.0
7	0.0	0.0	0.0	0.15408879140003262	0.0
8	0.0	0.0	0.0	0.33536972245889457	0.0
9	0.0	0.0	0.0	0.40865901315840586	0.0
10	0.0	0.0	0.0	0.5441017659352413	0.0
11	0.0	0.0	0.0	0.6798034914707322	0.0
12	0.0	0.0	0.0	0.7877951318300828	0.0
13	0.0	0.0	0.0	0.8426973566650524	0.0
14	0.0	0.0	0.0	0.8592716132190055	0.0
15	0.0	0.0	0.0	0.8887945077057344	0.0
16	0.0	0.0	0.0	0.9532787246109582	0.0
17	0.0	0.0	0.0	1.033301307035513	0.0
18	0.0	0.0	0.0	1.1537236398103283	0.0
19	0.0	0.0	0.0	1.2057771643000872	0.0
20	0.0	0.0	0.0	1.2547230156859799	0.0
21	0.0	0.0	0.0	1.3241277150056585	0.0
22	0.0	0.0	0.0	1.3906837139801265	0.0
23	0.0	0.0	0.0	1.4776985608883801	0.0
24	0.0	0.0	0.0	1.5403699684830152	0.0
25	0.0	0.0	0.0	1.5856902012477307	0.0
26	0.0	0.0	0.0	1.6374847529788341	0.0
27	0.0	0.0	0.0	1.6848767678127938	0.0
28	0.0	0.0	0.0	1.734599537474653	0.0
29	0.0	0.0	0.0	1.7902786805855893	0.0
30	0.0	0.0	0.0	1.862791053009134	0.0
31	0.0	0.0	0.0	1.9332316433634347	0.0
32	0.0	0.0	0.0	1.9948671599234475	0.0
33	0.0	0.0	0.0	2.0523591123449725	0.0
34	0.0	0.0	0.0	2.1186561385607847	0.0
35	0.0	0.0	0.0	2.221468323747025	0.0
36	0.0	0.0	0.0	2.3090011161725896	0.0
37	0.0	0.0	0.0	2.3789237610095793	0.0
38	0.0	0.0	0.0	2.446256678260014	0.0
39	0.0	0.0	0.0	2.5112588406825487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	25	3.885977E-5	45.000004	1
TTAGGAC	65	0.0	45.000004	5
TCACCGG	25	3.885977E-5	45.000004	17
CATGATA	25	3.885977E-5	45.000004	1
CGGTTTA	25	3.885977E-5	45.000004	1
TTGTAGC	25	3.885977E-5	45.000004	34
ATCACGG	25	3.885977E-5	45.000004	2
ATCGTCC	25	3.885977E-5	45.000004	19
TTGCGAA	25	3.885977E-5	45.000004	1
GCGATAA	65	0.0	45.000004	9
TTCATAG	25	3.885977E-5	45.000004	2
ATTGCGA	25	3.885977E-5	45.000004	1
TTACCGG	25	3.885977E-5	45.000004	2
CCGCAAT	50	2.1827873E-11	45.000004	33
GCGTAAA	25	3.885977E-5	45.000004	21
CTTGTAT	20	7.0274604E-4	45.0	18
GTGCATC	20	7.0274604E-4	45.0	15
CGGGTTC	20	7.0274604E-4	45.0	6
CTTCGCA	40	6.7939254E-9	45.0	24
AACGTCA	20	7.0274604E-4	45.0	34
>>END_MODULE