FastQCFastQC Report
Thu 26 May 2016
SRR1546427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546427_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2806407
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99070.3530136576768801No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA71370.25431093921872344No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.15471740200191916No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.8584254E-444.038
TAGCGTC351.4470788E-744.040
TAAGTCG207.8584254E-444.034
CGCTCGT207.8584254E-444.016
GTCGTAC254.444487E-544.032
ACGTCGA254.444487E-544.01
CGACTCA254.444487E-544.014
CGTTATT35250.043.5631261
CGTTTTA60550.043.2733271
TGTTACG1050.041.904761
TTAACGG2700.041.5555532
AGGCGAT24250.041.3690727
CGACGGT2250.041.06666628
GTTAACG700.040.8571431
TTACGGG6600.040.3333323
GTTATTT40700.039.6756742
TACCCGT501.3496901E-939.616
CCGTTGA501.3496901E-939.632
CACGACG2350.039.31915326
TTTACGG2750.039.22
Produced by FastQC (version 0.11.5)