FastQCFastQC Report
Thu 26 May 2016
SRR1546426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546426_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1100935
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.25060516742586986No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27080.24597274135166927No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.22989549791767905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCG1100.045.0000047
TACCCGT207.033157E-445.016
CGTCTAT207.033157E-445.034
ACTACGG207.033157E-445.02
ACCCGTT207.033157E-445.017
CGCACTC207.033157E-445.011
CGGTAAC207.033157E-445.034
TGTACGC207.033157E-445.013
GCGTCCA207.033157E-445.037
TTCGAAT207.033157E-445.014
CGTTTTA18300.043.647541
AGGCGAT8800.043.465917
CGTTTTT23550.042.898091
CGTTATT4250.041.8235321
CGGTTAG556.184564E-1140.9090922
TAAGGAT7500.040.25
GTTAGCG451.9281288E-840.01
CGGTCTA451.9281288E-840.031
GCGATAT1300.039.8076939
CGTTATA403.4580444E-739.37535