FastQCFastQC Report
Thu 26 May 2016
SRR1546425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546425_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences507006
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20080.39605053983582045No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.28835950659361037No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.14457422594604405No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.12130033964095098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC207.852312E-444.0000049
GTCGATG207.852312E-444.0000041
CAAGTGC207.852312E-444.00000442
CACGGTC207.852312E-444.00000423
ACGTTAG207.852312E-444.0000042
ACACCCG207.852312E-444.00000413
ATGTGCG207.852312E-444.00000432
AGCTTAC207.852312E-444.00000425
TAGCGCA207.852312E-444.0000041
CTCCCTA207.852312E-444.00000429
CAACCGG207.852312E-444.00000415
CAACCGA207.852312E-444.00000438
ATTAACG207.852312E-444.0000041
TAAGTTA207.852312E-444.00000416
GTAGGCT408.296411E-944.0000045
CGATTAC207.852312E-444.00000410
TAACGCT207.852312E-444.00000436
GCGTTAT207.852312E-444.00000429
TCGATAG207.852312E-444.0000042
AATATCG207.852312E-444.00000412
Produced by FastQC (version 0.11.5)