##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546425_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	507006
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.660260430843028	31.0	31.0	33.0	30.0	34.0
2	31.839842132045774	31.0	31.0	34.0	30.0	34.0
3	31.87252813576171	31.0	31.0	34.0	30.0	34.0
4	35.7623479801028	37.0	35.0	37.0	35.0	37.0
5	27.349216774554936	35.0	28.0	37.0	0.0	37.0
6	31.496394519985955	36.0	29.0	37.0	17.0	37.0
7	14.737902510029468	0.0	0.0	32.0	0.0	37.0
8	24.986262489990256	17.0	17.0	35.0	17.0	37.0
9	33.32463126669112	32.0	32.0	37.0	28.0	39.0
10	36.30029033186984	37.0	35.0	39.0	33.0	39.0
11	37.25952355593425	39.0	37.0	39.0	34.0	39.0
12	36.9076934000781	39.0	35.0	39.0	33.0	39.0
13	36.15793698693901	38.0	35.0	39.0	32.0	39.0
14	37.39736807848428	40.0	35.0	41.0	33.0	41.0
15	37.79974990434038	40.0	35.0	41.0	33.0	41.0
16	37.95020966221307	40.0	35.0	41.0	33.0	41.0
17	38.017027411904394	40.0	35.0	41.0	34.0	41.0
18	37.986868005506835	40.0	36.0	41.0	34.0	41.0
19	37.902468609838934	40.0	36.0	41.0	34.0	41.0
20	37.89202889117683	40.0	35.0	41.0	34.0	41.0
21	37.73121422626162	40.0	35.0	41.0	34.0	41.0
22	37.66116969029952	39.0	35.0	41.0	33.0	41.0
23	37.56428129055672	39.0	35.0	41.0	34.0	41.0
24	37.42870104101332	39.0	35.0	41.0	33.0	41.0
25	37.24785308260652	39.0	35.0	41.0	33.0	41.0
26	36.99975148223098	38.0	35.0	40.0	33.0	41.0
27	36.799738070160906	38.0	35.0	40.0	33.0	41.0
28	36.85309049597046	38.0	35.0	40.0	33.0	41.0
29	36.93553133493489	38.0	35.0	40.0	33.0	41.0
30	36.77979156065214	38.0	35.0	40.0	33.0	41.0
31	36.53974903650055	38.0	35.0	40.0	32.0	41.0
32	36.210374236202334	38.0	35.0	40.0	31.0	41.0
33	36.015104357739354	38.0	35.0	40.0	31.0	41.0
34	35.83759166558187	38.0	35.0	40.0	30.0	41.0
35	35.72891445071656	38.0	35.0	40.0	30.0	41.0
36	35.52161710117829	38.0	35.0	40.0	30.0	41.0
37	35.25889437205871	37.0	34.0	40.0	29.0	41.0
38	35.14240265401198	37.0	34.0	40.0	29.0	41.0
39	35.155692437564845	37.0	34.0	40.0	29.0	41.0
40	34.91400299010268	37.0	34.0	40.0	28.0	41.0
41	34.82592316461738	37.0	34.0	40.0	27.0	40.0
42	34.82158396547575	37.0	34.0	40.0	27.0	41.0
43	34.680678335167634	37.0	34.0	40.0	27.0	41.0
44	34.4674007802669	36.0	34.0	40.0	27.0	40.0
45	34.23314516987965	36.0	33.0	39.0	26.0	40.0
46	34.2659771284758	36.0	34.0	39.0	26.0	40.0
47	34.15892908565185	36.0	33.0	39.0	26.0	40.0
48	34.20642950970994	36.0	33.0	39.0	26.0	40.0
49	34.11517023467178	36.0	33.0	39.0	26.0	40.0
50	34.12904186538226	36.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	8.0
16	15.0
17	49.0
18	125.0
19	243.0
20	561.0
21	913.0
22	1625.0
23	2636.0
24	3992.0
25	5920.0
26	7739.0
27	8489.0
28	8524.0
29	9510.0
30	11871.0
31	16942.0
32	24877.0
33	53207.0
34	47822.0
35	53823.0
36	60215.0
37	120521.0
38	56154.0
39	11221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.54717301175923	23.09499295866321	29.624501485189526	16.733332544388034
2	30.36433493883702	24.97524684126026	31.70475300095068	12.955665218952046
3	27.54641956899918	24.4062200447332	34.6108330078934	13.436527378374223
4	25.918430945590387	26.559054527954306	32.53077872845686	14.991735797998446
5	17.660737742748605	47.36472546675976	24.14507915093707	10.829457639554562
6	21.752405296978733	38.81137501331345	28.31307716279492	11.123142526912897
7	37.875291416669626	57.08512325297925	3.972339577835371	1.0672457525157493
8	87.88909795939298	2.9983866068646132	7.45474412531607	1.6577713084263301
9	84.2627108949401	4.317305909594758	9.22020646698461	2.199776728480531
10	63.69924616276731	15.548533942399104	12.659021786724416	8.093198108109174
11	59.91290043904806	15.198439466199613	16.01913981294107	8.86952028181126
12	52.55953578458637	18.990110570683584	19.23665597645787	9.213697668272172
13	20.567014986015945	48.64064725072287	19.944142672867777	10.848195090393407
14	12.48091738559307	50.47356441541125	26.242884699589354	10.802633499406317
15	10.393368125820997	21.005274099320324	57.99872190861647	10.602635866242215
16	12.545019191094386	13.049549709470895	56.96244225906597	17.442988840368752
17	12.91897926257283	14.676552151256592	29.06099730575181	43.34347128041877
18	21.076476412507937	21.73287890084141	35.669203125801275	21.52144156084938
19	31.155449836885555	22.329124310165955	25.880956043912697	20.634469809035792
20	35.62009128097103	20.875492597720736	23.025565772397172	20.47885034891106
21	21.32262734563299	27.81840846064938	28.20558336587733	22.6533808278403
22	22.59519611207757	23.99143205405853	25.854526376413688	27.55884545745021
23	19.862092361826093	31.81402192478984	23.01195646599843	25.311929247385635
24	21.032492712117808	22.09461032019345	41.323771316315785	15.549125651372961
25	14.875366366472981	23.083553251835284	40.27999668642975	21.76108369526199
26	14.782270821252608	37.72440562833576	28.438519465252877	19.054804085158754
27	16.628205583365876	35.04850041222392	30.658414298844587	17.664879705565614
28	12.863555855354768	27.352339025573663	45.375991605622026	14.408113513449544
29	12.647976552545728	21.88909006994	44.970473722204474	20.4924596553098
30	16.424657696358622	29.367305317885783	34.76704417699199	19.440992808763603
31	32.32999214999428	24.994970473722205	23.937586537437426	18.73745083884609
32	33.09053541772681	26.740117473954943	24.966765679301623	15.202581429016618
33	28.932399222099935	28.262781900016964	25.01646923310572	17.78834964477738
34	18.720488514928817	27.72511567910439	29.646591953546903	23.907803852419892
35	19.248490155935038	25.78628260809537	32.70099367660343	22.26423355936616
36	32.90434432728607	21.174700102168416	27.844049182849908	18.076906387695608
37	21.390673877626696	31.750314591937766	29.919961499469434	16.9390500309661
38	20.786341778992753	33.3664690358694	22.44608545066528	23.40110373447257
39	21.21197776752149	31.501007877618804	27.181137895803992	20.10587645905571
40	23.716287381214425	25.970698571614538	24.983728003218896	25.32928604395214
41	16.490337392456894	23.14509098511655	27.226107777817226	33.13846384460933
42	23.973483548518164	25.519619097209894	22.3407612533185	28.166136100953445
43	24.472885922454566	28.59847812451924	22.862845804586136	24.06579014844006
44	20.618296430416997	33.28915239661858	25.632241038567592	20.46031013439683
45	15.897445000650881	40.88728732993298	20.838017696043046	22.377249973373097
46	22.8482503165643	34.21064050524057	25.221595010709937	17.719514167485197
47	24.06145094929843	26.47739868956186	26.822562257645867	22.638588103493845
48	26.388642343483117	20.253409229871046	30.649933136885956	22.708015289759885
49	19.731719151252648	21.34077308749798	34.06034642588056	24.867161335368813
50	17.27159047427447	35.43606979010111	26.17562711289413	21.1167126227303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	317.0
2	484.0
3	2598.0
4	4712.0
5	3595.0
6	2478.0
7	2772.5
8	3067.0
9	3218.5
10	3370.0
11	3351.5
12	3333.0
13	3238.0
14	3143.0
15	2889.0
16	2635.0
17	2370.0
18	2105.0
19	1914.0
20	1723.0
21	1671.0
22	1619.0
23	1825.0
24	2031.0
25	2411.5
26	2792.0
27	3311.0
28	3830.0
29	4479.5
30	5129.0
31	6372.5
32	7616.0
33	8178.0
34	8740.0
35	9441.0
36	10142.0
37	13237.5
38	16333.0
39	26772.0
40	37211.0
41	48332.5
42	59454.0
43	60526.5
44	61599.0
45	56746.0
46	51893.0
47	46503.0
48	41113.0
49	39699.5
50	38286.0
51	35826.5
52	33367.0
53	28915.5
54	24464.0
55	21104.0
56	17744.0
57	16250.5
58	14757.0
59	14497.0
60	14237.0
61	12445.5
62	10654.0
63	8559.5
64	6465.0
65	5223.5
66	3982.0
67	3331.0
68	2680.0
69	2180.0
70	1680.0
71	1349.0
72	1018.0
73	762.5
74	507.0
75	351.0
76	195.0
77	177.0
78	159.0
79	119.0
80	79.0
81	47.0
82	15.0
83	13.5
84	12.0
85	6.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	507006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.83576257665146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00776078400898	14.084930840112644
2	5.274567826540276	2.0925015020339486
3	2.287948616343872	1.361496166241258
4	1.6789018560860254	1.3320919442728743
5	1.2999224476379552	1.2892476519703058
6	1.1959286038330266	1.4233293506554907
7	1.0519371986176165	1.4606183562128823
8	0.9909408400983157	1.5724853785358186
9	0.9489433473783022	1.6940713443560005
>10	13.191212474830264	59.06082917630572
>50	1.0259387506955615	12.58365159817026
>100	0.04199749271853175	1.0891167020089356
>500	0.001999880605644369	0.26737011733892646
>1k	0.001999880605644369	0.688259871784922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2008	0.39605053983582045	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	0.28835950659361037	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.14457422594604405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.12130033964095098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005325380764724678	0.0
2	0.0	0.0	0.0	0.027613085446720552	0.0
3	0.0	0.0	0.0	0.03609424740535615	0.0
4	0.0	0.0	0.0	0.05562064354267997	0.0
5	0.0	0.0	0.0	0.08402267428787825	0.0
6	0.0	0.0	0.0	0.12682295673029512	0.0
7	0.0	0.0	0.0	0.1522664426062019	0.0
8	0.0	0.0	0.0	0.31755048263728636	0.0
9	0.0	0.0	0.0	0.3897389774479986	0.0
10	0.0	0.0	0.0	0.5228734965661156	0.0
11	0.0	0.0	0.0	0.6601499785012406	0.0
12	0.0	0.0	0.0	0.7903259527500661	0.0
13	0.0	0.0	0.0	0.8396350339049242	0.0
14	0.0	0.0	0.0	0.8556111761990982	0.0
15	0.0	0.0	0.0	0.8820408436981022	0.0
16	0.0	0.0	0.0	0.9412117410839319	0.0
17	0.0	0.0	0.0	1.0116251089730695	0.0
18	0.0	0.0	0.0	1.114976943073652	0.0
19	0.0	0.0	0.0	1.1532407900498218	0.0
20	0.0	0.0	0.0	1.2005775079584857	0.0
21	0.0	0.0	0.0	1.25817051474736	0.0
22	0.0	0.0	0.0	1.3163552305100925	0.0
23	0.0	0.0	0.0	1.3885437253208048	0.0
24	0.0	0.0	0.0	1.439233460747999	0.0
25	0.0	0.0	0.0	1.478483489347266	0.0
26	0.0	0.0	0.0	1.5214810081143024	0.0
27	0.0	0.0	0.0	1.5571807828704196	0.0
28	0.0	0.0	0.0	1.5976142294174034	0.0
29	0.0	0.0	0.0	1.6368642580166703	0.0
30	0.0	0.0	0.0	1.6920904289101115	0.0
31	0.0	0.0	0.0	1.7540226348406134	0.0
32	0.0	0.0	0.0	1.8035289523200908	0.0
33	0.0	0.0	0.0	1.851457379202613	0.0
34	0.0	0.0	0.0	1.8972162065143214	0.0
35	0.0	0.0	0.0	1.9686157560265558	0.0
36	0.0	0.0	0.0	2.0266032354646693	0.0
37	0.0	0.0	0.0	2.0869575507982154	0.0
38	0.0	0.0	0.0	2.135674922979215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	20	7.852312E-4	44.000004	9
GTCGATG	20	7.852312E-4	44.000004	1
CAAGTGC	20	7.852312E-4	44.000004	42
CACGGTC	20	7.852312E-4	44.000004	23
ACGTTAG	20	7.852312E-4	44.000004	2
ACACCCG	20	7.852312E-4	44.000004	13
ATGTGCG	20	7.852312E-4	44.000004	32
AGCTTAC	20	7.852312E-4	44.000004	25
TAGCGCA	20	7.852312E-4	44.000004	1
CTCCCTA	20	7.852312E-4	44.000004	29
CAACCGG	20	7.852312E-4	44.000004	15
CAACCGA	20	7.852312E-4	44.000004	38
ATTAACG	20	7.852312E-4	44.000004	1
TAAGTTA	20	7.852312E-4	44.000004	16
GTAGGCT	40	8.296411E-9	44.000004	5
CGATTAC	20	7.852312E-4	44.000004	10
TAACGCT	20	7.852312E-4	44.000004	36
GCGTTAT	20	7.852312E-4	44.000004	29
TCGATAG	20	7.852312E-4	44.000004	2
AATATCG	20	7.852312E-4	44.000004	12
>>END_MODULE