FastQCFastQC Report
Thu 26 May 2016
SRR1546424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546424_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences181247
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8040.4435935491346064No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.2284175738081182No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.20248611011492607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG253.877765E-545.0000042
TAGGTGA253.877765E-545.0000046
AGGGTAT253.877765E-545.0000046
TATGCAG253.877765E-545.0000042
CGGGGAT253.877765E-545.0000046
TTTATGG253.877765E-545.0000042
TAAGGTG253.877765E-545.0000045
AGGTGTA253.877765E-545.0000047
CATATAG207.0175476E-445.02
CTACTAG207.0175476E-445.02
GGATGGT207.0175476E-445.08
TAGACTA207.0175476E-445.01
TTAGCGG207.0175476E-445.02
AACCCAC207.0175476E-445.030
TCACAAG351.2043711E-745.02
GTTAGAA207.0175476E-445.01
AAGGTAT351.2043711E-745.06
CTAAGGT207.0175476E-445.04
TGTTGCA207.0175476E-445.01
CACTCCT207.0175476E-445.032