##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546424_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	181247
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160984733540417	31.0	31.0	33.0	30.0	34.0
2	31.31796940087284	31.0	31.0	33.0	30.0	34.0
3	31.239992937814144	31.0	31.0	34.0	28.0	34.0
4	35.206480658990216	35.0	35.0	37.0	33.0	37.0
5	32.96118004711802	35.0	35.0	37.0	28.0	37.0
6	34.13710571761188	36.0	35.0	37.0	29.0	37.0
7	15.184996165453773	0.0	0.0	35.0	0.0	37.0
8	24.886889162303376	17.0	17.0	35.0	17.0	37.0
9	32.875418627618664	32.0	32.0	37.0	28.0	39.0
10	35.81939563137597	37.0	35.0	38.0	32.0	39.0
11	36.765060938939676	38.0	35.0	39.0	33.0	39.0
12	36.323172245609584	37.0	35.0	39.0	32.0	39.0
13	35.62044613152218	37.0	35.0	39.0	31.0	39.0
14	36.581460658659175	39.0	35.0	40.0	31.0	41.0
15	37.139511274669374	39.0	35.0	41.0	32.0	41.0
16	37.330615127422796	39.0	35.0	41.0	33.0	41.0
17	37.35489690863849	39.0	35.0	41.0	33.0	41.0
18	37.2493613687399	39.0	35.0	41.0	32.0	41.0
19	37.13510844317423	39.0	35.0	41.0	32.0	41.0
20	37.21911535087477	39.0	35.0	41.0	32.0	41.0
21	37.03826821961191	39.0	35.0	41.0	32.0	41.0
22	37.08509933957527	39.0	35.0	41.0	32.0	41.0
23	37.09957682058186	38.0	35.0	41.0	33.0	41.0
24	36.92899192814226	38.0	35.0	41.0	32.0	41.0
25	36.676590509084285	38.0	35.0	40.0	32.0	41.0
26	36.537664071681185	38.0	35.0	40.0	32.0	41.0
27	36.43586376602096	38.0	35.0	40.0	31.0	41.0
28	36.41223854739665	38.0	35.0	40.0	31.0	41.0
29	36.54828493712999	38.0	35.0	40.0	32.0	41.0
30	36.36329428900892	38.0	35.0	40.0	31.0	41.0
31	36.187147925207036	38.0	35.0	40.0	31.0	41.0
32	35.98800531870872	38.0	34.0	40.0	30.0	41.0
33	35.79443521823809	38.0	34.0	40.0	30.0	41.0
34	35.66385650521112	38.0	34.0	40.0	30.0	41.0
35	35.48878602128587	38.0	34.0	40.0	29.0	41.0
36	35.268087195926	38.0	34.0	40.0	27.0	41.0
37	35.032182601643065	37.0	34.0	40.0	27.0	41.0
38	35.04566696276352	37.0	34.0	40.0	27.0	41.0
39	34.991067438357604	37.0	34.0	40.0	27.0	41.0
40	34.796283524692825	37.0	34.0	40.0	25.0	41.0
41	34.78662819246664	37.0	34.0	40.0	25.0	41.0
42	34.747228919651086	37.0	34.0	40.0	25.0	41.0
43	34.63860367344011	37.0	33.0	40.0	25.0	41.0
44	34.41677931220931	36.0	33.0	40.0	24.0	41.0
45	34.30633886353981	36.0	33.0	40.0	24.0	41.0
46	34.35608865250183	36.0	34.0	40.0	24.0	41.0
47	34.36870679238829	36.0	33.0	40.0	24.0	41.0
48	34.41209509674643	36.0	33.0	40.0	24.0	41.0
49	34.36036458534486	36.0	34.0	40.0	24.0	41.0
50	34.213189735554245	36.0	34.0	40.0	24.0	41.0
51	34.000303453298535	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	9.0
16	17.0
17	38.0
18	102.0
19	194.0
20	286.0
21	477.0
22	780.0
23	1180.0
24	1824.0
25	2560.0
26	3024.0
27	3278.0
28	3346.0
29	4002.0
30	4795.0
31	6645.0
32	9230.0
33	15693.0
34	20077.0
35	18614.0
36	19178.0
37	31238.0
38	29991.0
39	4667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.967392563738986	23.782462606277623	29.767665119974403	16.48247971000899
2	29.824493646791396	24.661373705495816	32.4866066748691	13.027525972843687
3	28.00763598845774	23.980534850232004	34.55836510397414	13.45346405733612
4	26.351332711713848	27.22251954515109	31.315828675784978	15.110319067350082
5	21.470148471422977	36.01714787003371	29.243518513409878	13.269185145133436
6	21.680358847318853	39.31706455830993	27.89839280098429	11.104183793386925
7	37.2574442611464	57.62633312551381	4.3255888373324805	0.790633776007327
8	88.35291066886624	3.122810308584418	6.9303216053231225	1.593957417226216
9	84.59340016662344	4.462418688309324	8.610349412679934	2.333831732387295
10	63.89402307348535	15.848538182700956	12.134821541873798	8.122617201939894
11	60.44624186883093	15.34866784001942	15.574878480747268	8.630211810402379
12	53.142396839671825	19.088315944539772	18.665136526397678	9.104150689390721
13	20.627651768029263	49.32054047791136	19.17880020083091	10.873007553228467
14	12.403515644396872	51.33326344711912	25.632424260815352	10.630796647668651
15	10.241272959000701	21.02655492228837	58.16813519672049	10.564036921990432
16	12.4597924379438	13.139527826667477	56.972529200483315	17.428150534905406
17	12.651795615927435	14.847142297527682	28.6669572461889	43.834104840355984
18	20.93882933234757	21.678703647508648	36.229013445739795	21.15345357440399
19	32.0893587204202	21.867948159141946	25.629113861194945	20.413579259242912
20	36.34708436553433	20.826275745253714	22.59016700966085	20.23647287955111
21	21.360905283949528	28.34253808338897	27.85425413937886	22.442302493282646
22	22.31981770732757	24.202883358069375	25.347729893460308	28.129569041142748
23	19.681429209862785	32.24770616892969	22.48699288815815	25.583871733049374
24	20.445028055636783	22.05774440404531	42.056971977467214	15.440255562850696
25	14.693208715178734	22.99238056354036	40.91102197553615	21.403388745744756
26	14.4427218105679	38.76367608843181	28.100327177829147	18.693274923171142
27	16.407443985279755	36.34101529956358	30.040773088658018	17.210767626498647
28	12.622002019343768	27.983359724574754	45.31606040375841	14.078577852323074
29	12.562966559446501	22.274575579182002	44.82887992628844	20.333577935083063
30	16.3533741248131	30.36077838529741	34.15891021644496	19.126937273444526
31	32.93737275651459	25.26165950332971	23.62080475814772	18.180162982007978
32	34.38787952352315	26.167053799511166	24.760133960837972	14.684932716127715
33	29.927667768294096	27.95963519396183	24.387162270272057	17.725534767472013
34	19.04914288236495	27.551352574111572	28.994686808609245	24.404817734914232
35	19.272043123472386	25.56952666802761	32.951717821536356	22.206712386963645
36	33.66290200665391	21.60366792277941	27.24679580903408	17.486634261532604
37	21.230696232213496	32.968269819638394	29.4614531550867	16.339580793061405
38	20.110677693975624	34.85023200384006	21.942983883871182	23.09610641831313
39	21.260489828797166	32.28908616418479	27.031619833707595	19.418804173310455
40	24.47157746059245	25.672149056260242	24.610614244649568	25.24565923849774
41	16.180129878011773	23.20810827213692	26.953825442628016	33.657936407223296
42	23.6985991492273	24.95379233863181	22.267403046671117	29.08020546546977
43	24.166468962244892	27.887909868853	23.063002422109054	24.88261874679305
44	20.285577140587154	32.919165558602344	26.495886828471644	20.29937047233885
45	15.529636352601697	41.95159092288424	20.841724276815615	21.677048447698443
46	22.578028877719355	35.17740983299034	24.825238486705985	17.41932280258432
47	23.704116481927976	26.724856135549828	26.439610034924716	23.131417347597477
48	25.918222094710536	20.582961373153765	31.361622537200617	22.13719399493509
49	19.911501983481106	21.74932550607734	34.418776586646956	23.9203959237946
50	17.375735874248953	36.65660673004243	25.684287188201736	20.28337020750688
51	17.65491290890332	38.818297682168534	21.144074108812834	22.382715300115315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	107.5
2	158.0
3	807.5
4	1457.0
5	1155.0
6	853.0
7	905.5
8	958.0
9	997.5
10	1037.0
11	1038.0
12	1039.0
13	1006.5
14	974.0
15	922.0
16	870.0
17	779.0
18	688.0
19	628.0
20	568.0
21	568.5
22	569.0
23	621.0
24	673.0
25	763.5
26	1082.0
27	1310.0
28	1483.5
29	1657.0
30	2087.5
31	2518.0
32	2717.0
33	2916.0
34	3255.5
35	3595.0
36	4484.5
37	5374.0
38	7890.0
39	10406.0
40	14847.5
41	19289.0
42	21085.5
43	22882.0
44	20897.5
45	18913.0
46	17403.5
47	15894.0
48	14785.0
49	13676.0
50	12785.0
51	11894.0
52	10877.5
53	9861.0
54	8583.5
55	7306.0
56	6672.5
57	6039.0
58	5626.0
59	5213.0
60	4790.5
61	4368.0
62	3564.5
63	2761.0
64	2180.5
65	1600.0
66	1366.5
67	1133.0
68	925.0
69	717.0
70	626.0
71	535.0
72	441.5
73	348.0
74	246.5
75	104.5
76	64.0
77	53.0
78	42.0
79	29.5
80	17.0
81	13.5
82	10.0
83	9.0
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	181247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.621422699410196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.50198749575535	17.540152388729194
2	7.073087909234364	3.9073750186209977
3	4.152767512933703	3.4411604054136067
4	3.241915186864551	3.581852389280926
5	2.536803627429439	3.5035062649312816
6	2.2152088368655494	3.671233179031929
7	1.9475460919241756	3.765579568213543
8	1.8217046521383058	4.025445938415532
9	1.7418053252901344	4.330002703493023
>10	11.737211113996365	50.85656590178045
>50	0.017977348540838545	0.30179809872715135
>100	0.009987415856021413	0.6317345942277665
>500	0.0019974831712042825	0.4435935491346064
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	804	0.4435935491346064	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	414	0.2284175738081182	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.20248611011492607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004965599430611266	0.0
2	0.0	0.0	0.0	0.031448796393871346	0.0
3	0.0	0.0	0.0	0.039724795444890125	0.0
4	0.0	0.0	0.0	0.05517332700679184	0.0
5	0.0	0.0	0.0	0.08055305742991609	0.0
6	0.0	0.0	0.0	0.108139720933312	0.0
7	0.0	0.0	0.0	0.1263469188455533	0.0
8	0.0	0.0	0.0	0.26924583579314415	0.0
9	0.0	0.0	0.0	0.33490209493122647	0.0
10	0.0	0.0	0.0	0.4380762164339272	0.0
11	0.0	0.0	0.0	0.5456642040971713	0.0
12	0.0	0.0	0.0	0.6477348590597362	0.0
13	0.0	0.0	0.0	0.6775284556434038	0.0
14	0.0	0.0	0.0	0.6918735206651696	0.0
15	0.0	0.0	0.0	0.7178049843583618	0.0
16	0.0	0.0	0.0	0.7608401794236594	0.0
17	0.0	0.0	0.0	0.8292551049120813	0.0
18	0.0	0.0	0.0	0.9120150954222691	0.0
19	0.0	0.0	0.0	0.9401534921957329	0.0
20	0.0	0.0	0.0	0.980430020910691	0.0
21	0.0	0.0	0.0	1.0449828135086374	0.0
22	0.0	0.0	0.0	1.0830524091433238	0.0
23	0.0	0.0	0.0	1.1404326692303872	0.0
24	0.0	0.0	0.0	1.175191865244666	0.0
25	0.0	0.0	0.0	1.2000198623977225	0.0
26	0.0	0.0	0.0	1.2342273251419333	0.0
27	0.0	0.0	0.0	1.2761590536670953	0.0
28	0.0	0.0	0.0	1.3076078500609665	0.0
29	0.0	0.0	0.0	1.3445739791555171	0.0
30	0.0	0.0	0.0	1.3964369065419013	0.0
31	0.0	0.0	0.0	1.452161966818761	0.0
32	0.0	0.0	0.0	1.4885763626432438	0.0
33	0.0	0.0	0.0	1.5299563578983377	0.0
34	0.0	0.0	0.0	1.5691294200731598	0.0
35	0.0	0.0	0.0	1.628716613240495	0.0
36	0.0	0.0	0.0	1.6745104746561323	0.0
37	0.0	0.0	0.0	1.7385115339840107	0.0
38	0.0	0.0	0.0	1.78375366212958	0.0
39	0.0	0.0	0.0	1.8367200560561003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	25	3.877765E-5	45.000004	2
TAGGTGA	25	3.877765E-5	45.000004	6
AGGGTAT	25	3.877765E-5	45.000004	6
TATGCAG	25	3.877765E-5	45.000004	2
CGGGGAT	25	3.877765E-5	45.000004	6
TTTATGG	25	3.877765E-5	45.000004	2
TAAGGTG	25	3.877765E-5	45.000004	5
AGGTGTA	25	3.877765E-5	45.000004	7
CATATAG	20	7.0175476E-4	45.0	2
CTACTAG	20	7.0175476E-4	45.0	2
GGATGGT	20	7.0175476E-4	45.0	8
TAGACTA	20	7.0175476E-4	45.0	1
TTAGCGG	20	7.0175476E-4	45.0	2
AACCCAC	20	7.0175476E-4	45.0	30
TCACAAG	35	1.2043711E-7	45.0	2
GTTAGAA	20	7.0175476E-4	45.0	1
AAGGTAT	35	1.2043711E-7	45.0	6
CTAAGGT	20	7.0175476E-4	45.0	4
TGTTGCA	20	7.0175476E-4	45.0	1
CACTCCT	20	7.0175476E-4	45.0	32
>>END_MODULE