FastQCFastQC Report
Thu 26 May 2016
SRR1546423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546423_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1860366
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82790.44501995843828585No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA77050.41416581468377733No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.18474859248126446No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.17915829465814792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.8577385E-444.00000440
CTATCGA207.8577385E-444.00000438
ACACCGC207.8577385E-444.00000440
CGCGACA207.8577385E-444.00000436
CCTCGAA207.8577385E-444.00000441
CGCCTCG207.8577385E-444.00000434
ATATCGT207.8577385E-444.00000438
CCCGTAC207.8577385E-444.00000443
CCGTAGT207.8577385E-444.00000410
GTTCGCC207.8577385E-444.00000437
TCTCGTC408.3164196E-944.00000442
ACGTGGT207.8577385E-444.00000419
TTGCGAT207.8577385E-444.00000437
CTCGACT207.8577385E-444.00000442
CGTAAAT207.8577385E-444.00000442
TTACGGA207.8577385E-444.0000041
AATCGAC207.8577385E-444.00000442
GGTACGT502.7284841E-1144.08
TGGACCG254.4439053E-544.014
ATTACGG950.044.02