##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546423_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1860366 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54574261193765 31.0 31.0 33.0 30.0 34.0 2 31.717940448277382 31.0 31.0 34.0 30.0 34.0 3 31.732043587122103 31.0 31.0 34.0 30.0 34.0 4 35.63321625959623 37.0 35.0 37.0 33.0 37.0 5 26.97366485949539 35.0 19.0 37.0 0.0 37.0 6 31.26371047417551 35.0 25.0 37.0 17.0 37.0 7 15.147178028409463 0.0 0.0 32.0 0.0 37.0 8 25.194695022377317 17.0 17.0 35.0 17.0 37.0 9 33.28301474011028 32.0 32.0 37.0 28.0 39.0 10 36.123615460613664 37.0 35.0 39.0 32.0 39.0 11 37.07783683425735 39.0 37.0 39.0 34.0 39.0 12 36.87712256620471 39.0 35.0 39.0 33.0 39.0 13 36.2560146766819 39.0 35.0 39.0 32.0 39.0 14 37.55650017254669 40.0 35.0 41.0 33.0 41.0 15 37.89707777931869 40.0 36.0 41.0 33.0 41.0 16 38.01611242088922 40.0 35.0 41.0 33.0 41.0 17 38.051853237481225 40.0 35.0 41.0 34.0 41.0 18 38.00710935375082 40.0 36.0 41.0 34.0 41.0 19 37.924504102956085 40.0 36.0 41.0 34.0 41.0 20 37.90865184592709 40.0 36.0 41.0 34.0 41.0 21 37.75224821352357 40.0 35.0 41.0 33.0 41.0 22 37.64674263021362 39.0 35.0 41.0 33.0 41.0 23 37.536002055509506 39.0 35.0 41.0 33.0 41.0 24 37.37203163248522 39.0 35.0 41.0 33.0 41.0 25 37.19889204597375 39.0 35.0 41.0 33.0 41.0 26 36.938098739710355 38.0 35.0 40.0 33.0 41.0 27 36.69347590742897 38.0 35.0 40.0 32.0 41.0 28 36.742855975652105 38.0 35.0 40.0 32.0 41.0 29 36.74462121969548 38.0 35.0 40.0 32.0 41.0 30 36.543136135577626 38.0 35.0 40.0 32.0 41.0 31 36.28518581827447 38.0 35.0 40.0 31.0 41.0 32 35.94731574324622 38.0 35.0 40.0 30.0 41.0 33 35.65472654305658 38.0 35.0 40.0 30.0 41.0 34 35.36965844355358 38.0 34.0 40.0 29.0 41.0 35 35.166828462786356 38.0 34.0 40.0 27.0 41.0 36 34.94289725785141 38.0 34.0 40.0 25.0 41.0 37 34.72650381699085 38.0 34.0 40.0 24.0 41.0 38 34.593919153542906 37.0 34.0 40.0 24.0 41.0 39 34.56728837228803 37.0 34.0 40.0 24.0 41.0 40 34.321665736742126 37.0 34.0 40.0 23.0 40.0 41 34.175954086453956 37.0 34.0 40.0 23.0 40.0 42 34.190615717552355 37.0 33.0 40.0 23.0 40.0 43 34.02871908000899 37.0 33.0 40.0 23.0 41.0 44 33.8239679718937 36.0 33.0 40.0 22.0 40.0 45 33.56658420977377 36.0 33.0 39.0 21.0 40.0 46 33.551176488927446 36.0 33.0 39.0 22.0 40.0 47 33.42714014339114 36.0 33.0 39.0 21.0 40.0 48 33.424693850564886 36.0 33.0 39.0 21.0 40.0 49 33.34601954669135 36.0 33.0 39.0 20.0 40.0 50 33.34475474180887 36.0 33.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 2.0 14 9.0 15 39.0 16 117.0 17 310.0 18 766.0 19 1671.0 20 3156.0 21 5158.0 22 8625.0 23 13193.0 24 20554.0 25 30260.0 26 37724.0 27 39126.0 28 37644.0 29 39920.0 30 47361.0 31 63105.0 32 88067.0 33 169987.0 34 162823.0 35 189607.0 36 225553.0 37 431158.0 38 199244.0 39 45181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.10887857550611 22.952526545851732 28.067541548275987 17.871053330366173 2 32.94222749716991 24.463035768230554 29.878368020056268 12.716368714543266 3 27.223299071258023 23.56649175484824 36.07456812261673 13.135641051277005 4 23.869281635979156 26.677008717639435 34.89845546521491 14.555254181166502 5 17.03256240976238 47.116320121954494 25.444885576279074 10.406231892004046 6 20.906370036863713 37.42381875394412 31.09108637762677 10.578724831565403 7 38.13265776734255 55.455431888133845 5.323092337744293 1.0888180067793112 8 84.90608837185802 3.286181321309893 10.058235852515043 1.7494944543170539 9 81.1444629712648 4.711384749022504 11.722962040802724 2.421190238909978 10 54.545342153103206 20.4674241520217 16.17160279213875 8.815630902736343 11 50.54387147475282 17.626477800604828 21.55102813102368 10.278622593618675 12 44.08723874764428 20.21741958302829 24.56038220436194 11.134959464965496 13 19.78519280614675 43.096734728542664 25.059800060848243 12.058272404462347 14 13.260240189296086 44.42641931748914 29.984637431559168 12.32870306165561 15 11.212471094397554 21.896712797374278 54.93773805799505 11.953078050233126 16 12.851288402389638 16.50202164520315 52.92383326721731 17.722856685189903 17 14.266977573230214 16.687469024912303 32.1672187085767 36.878334693280785 18 20.687595881670596 22.068936972617216 37.222030503675086 20.021436642037106 19 28.195258352388723 22.53126535316169 29.190761387812934 20.08271490663665 20 31.43816861843315 20.352231765147287 28.15005219403064 20.059547422388928 21 20.996621094988836 26.05299172313405 30.939772066356834 22.01061511552028 22 21.57989341882189 23.574715942991865 28.681237992954074 26.164152645232175 23 17.865516785406744 29.273379539294957 27.740294114168933 25.12080956112937 24 19.557710687036852 21.583656119279755 42.89962297741412 15.95901021626927 25 15.130356069719614 23.89889946386894 39.927895908654534 21.042848557756916 26 14.835951635323372 34.63721654771158 30.954607856733567 19.57222396023148 27 16.352266166980044 33.8810212614077 33.17825632160553 16.58845625000672 28 13.609311286058764 27.585969642532703 43.32561442210834 15.47910464930019 29 13.690531863085006 23.44366646133073 42.234162525008514 20.631639150575747 30 17.083573877398315 29.227797110891085 35.51451703589509 18.174111975815514 31 28.049803103260324 26.00176524404338 27.671544201517335 18.27688745117896 32 29.64604814321483 26.407814376310895 28.46724784262882 15.478889637845455 33 27.536624513670965 27.27264420011976 27.32215058757255 17.86858069863672 34 18.78598082312835 27.562157123920777 30.706162120786985 22.945699932163883 35 19.761004017489032 25.898613498634138 32.31842551411926 22.021956969757564 36 30.228514174092624 24.542321242164174 27.884136777386814 17.345027806356384 37 20.16447301229973 31.54406175989026 30.04747452920554 18.243990698604467 38 19.511375718541405 33.13509277206743 24.733627684014866 22.619903825376298 39 21.19760305230261 30.932192912577417 28.819920381258314 19.050283653861662 40 23.620137112804684 26.26488551177564 26.16485143246006 23.95012594295961 41 16.724881018036236 24.481795517656202 28.458271114393618 30.335052349913944 42 22.978112908965226 25.58888949808801 24.921923965499264 26.5110736274475 43 23.38813975314535 27.001729767153343 25.75896355878359 23.851166920917713 44 19.227291833972455 32.15851074465992 27.96766872755146 20.646528693816162 45 16.81980857530185 37.96097112073646 22.724453145241313 22.49476715872038 46 22.32770325839109 32.582190816215736 26.260585282680935 18.829520642712243 47 21.819738696579062 27.581185637664845 28.00379065194698 22.59528501380911 48 23.89201909731741 23.460544860527442 30.744864182639333 21.902571859515817 49 20.60347265000543 23.655775261427053 32.24639667678296 23.49435541178456 50 18.044352562882786 33.589949504559854 27.44465336390796 20.921044568649393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 677.0 1 1335.5 2 1994.0 3 13621.0 4 25248.0 5 19370.0 6 13492.0 7 14480.5 8 15469.0 9 16629.0 10 17789.0 11 17824.0 12 17859.0 13 16787.0 14 15715.0 15 14592.0 16 13469.0 17 12971.5 18 12474.0 19 11642.5 20 10811.0 21 10842.0 22 10873.0 23 10964.5 24 11056.0 25 12149.5 26 13243.0 27 16315.0 28 19387.0 29 22009.0 30 24631.0 31 28329.5 32 32028.0 33 35836.5 34 39645.0 35 43312.5 36 46980.0 37 58120.5 38 69261.0 39 100318.0 40 131375.0 41 162628.5 42 193882.0 43 202660.5 44 211439.0 45 200576.0 46 189713.0 47 172871.0 48 156029.0 49 144722.5 50 133416.0 51 119057.0 52 104698.0 53 92138.0 54 79578.0 55 70472.0 56 61366.0 57 57599.0 58 53832.0 59 49856.5 60 45881.0 61 39386.0 62 32891.0 63 27136.0 64 21381.0 65 16877.5 66 12374.0 67 10409.0 68 8444.0 69 6995.5 70 5547.0 71 4122.5 72 2698.0 73 2368.5 74 2039.0 75 1565.5 76 1092.0 77 693.0 78 294.0 79 234.0 80 174.0 81 113.5 82 53.0 83 46.5 84 40.0 85 29.5 86 19.0 87 12.5 88 6.0 89 3.0 90 0.0 91 1.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1860366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.976304581755457 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9232134522351 15.166472416222684 2 6.847850616306286 2.5989379805077983 3 2.094112034929065 1.1921552340940098 4 1.0419447199064003 0.7908904144918292 5 0.6646089000542982 0.6305910457587918 6 0.48251310492652516 0.5493789386264563 7 0.3888688320957943 0.516550538014092 8 0.31869059659809523 0.48380558627094516 9 0.2961129881346385 0.5057217228110975 >10 5.048595943330151 25.03859155385882 >50 1.9236707191127091 25.83356193018745 >100 0.967106578599159 25.2047064581613 >500 0.00150639653986122 0.18775179423867774 >1k 6.02558615944488E-4 0.38705226766012435 >5k 6.02558615944488E-4 0.9138321190959272 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8279 0.44501995843828585 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 7705 0.41416581468377733 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3437 0.18474859248126446 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.17915829465814792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012846934420431248 0.0 2 0.0 0.0 0.0 0.0480550601333286 0.0 3 0.0 0.0 0.0 0.06520222364846487 0.0 4 0.0 0.0 0.0 0.09723893040401728 0.0 5 0.0 0.0 0.0 0.1600760280503944 0.0 6 0.0 0.0 0.0 0.2532297408144419 0.0 7 0.0 0.0 0.0 0.3164431085066057 0.0 8 0.0 0.0 0.0 0.6308436081932265 0.0 9 0.0 0.0 0.0 0.7418970245639837 0.0 10 0.0 0.0 0.0 0.968572850718622 0.0 11 0.0 0.0 0.0 1.1944423839180032 0.0 12 0.0 0.0 0.0 1.3975207029154477 0.0 13 0.0 0.0 0.0 1.4748710737564543 0.0 14 0.0 0.0 0.0 1.504542654509919 0.0 15 0.0 0.0 0.0 1.560821902786871 0.0 16 0.0 0.0 0.0 1.6881624368538233 0.0 17 0.0 0.0 0.0 1.8454433159926595 0.0 18 0.0 0.0 0.0 2.0397599182096426 0.0 19 0.0 0.0 0.0 2.1373751186594467 0.0 20 0.0 0.0 0.0 2.234399037608729 0.0 21 0.0 0.0 0.0 2.3817356369660594 0.0 22 0.0 0.0 0.0 2.5246107486376337 0.0 23 0.0 0.0 0.0 2.6910833674664016 0.0 24 0.0 0.0 0.0 2.8050931913397688 0.0 25 0.0 0.0 0.0 2.899375714241176 0.0 26 0.0 0.0 0.0 2.9957545988262524 0.0 27 0.0 0.0 0.0 3.091380943319755 0.0 28 0.0 0.0 0.0 3.1940489129558376 0.0 29 0.0 0.0 0.0 3.3026834504608233 0.0 30 0.0 0.0 0.0 3.441419591628744 0.0 31 0.0 0.0 0.0 3.5839184332545315 0.0 32 0.0 0.0 0.0 3.702389744813655 0.0 33 0.0 0.0 0.0 3.8229036651927633 0.0 34 0.0 0.0 0.0 3.9523943138070683 0.0 35 0.0 0.0 0.0 4.127628649416297 0.0 36 0.0 0.0 0.0 4.271417559770497 0.0 37 0.0 0.0 0.0 4.409616172301579 0.0 38 0.0 0.0 0.0 4.538945562324833 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACTA 20 7.8577385E-4 44.000004 40 CTATCGA 20 7.8577385E-4 44.000004 38 ACACCGC 20 7.8577385E-4 44.000004 40 CGCGACA 20 7.8577385E-4 44.000004 36 CCTCGAA 20 7.8577385E-4 44.000004 41 CGCCTCG 20 7.8577385E-4 44.000004 34 ATATCGT 20 7.8577385E-4 44.000004 38 CCCGTAC 20 7.8577385E-4 44.000004 43 CCGTAGT 20 7.8577385E-4 44.000004 10 GTTCGCC 20 7.8577385E-4 44.000004 37 TCTCGTC 40 8.3164196E-9 44.000004 42 ACGTGGT 20 7.8577385E-4 44.000004 19 TTGCGAT 20 7.8577385E-4 44.000004 37 CTCGACT 20 7.8577385E-4 44.000004 42 CGTAAAT 20 7.8577385E-4 44.000004 42 TTACGGA 20 7.8577385E-4 44.000004 1 AATCGAC 20 7.8577385E-4 44.000004 42 GGTACGT 50 2.7284841E-11 44.0 8 TGGACCG 25 4.4439053E-5 44.0 14 ATTACGG 95 0.0 44.0 2 >>END_MODULE