FastQCFastQC Report
Thu 26 May 2016
SRR1546422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546422_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences722442
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30030.41567350735422354No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.3221019818892036No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.2570448561960683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC502.1827873E-1145.00000425
GCGAATG302.1643773E-645.0000041
CATGCCG253.889361E-545.00000437
CGTTAAG253.889361E-545.0000042
AACATCG253.889361E-545.00000419
GGAACGT351.2110831E-745.0000048
AACGACC253.889361E-545.00000429
ATGCGGC253.889361E-545.00000419
AATAGGC600.045.0000044
CCCGGTT253.889361E-545.00000444
TCCGACA207.031546E-445.023
TAGGTTC207.031546E-445.032
CGCATGA207.031546E-445.038
CGTTGGC207.031546E-445.030
CGGCAAC207.031546E-445.08
CGACACA207.031546E-445.025
TTATCGC406.8084773E-945.031
CATTATC207.031546E-445.017
ACGATGT207.031546E-445.045
TCGTGAA207.031546E-445.01