##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546422_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	722442
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.010982196494666	31.0	31.0	33.0	30.0	33.0
2	31.157928248911333	31.0	31.0	33.0	30.0	34.0
3	31.06436779699962	31.0	31.0	34.0	28.0	34.0
4	35.011700593265616	35.0	35.0	37.0	32.0	37.0
5	32.727684713790175	35.0	35.0	37.0	28.0	37.0
6	33.94243274892656	35.0	35.0	37.0	28.0	37.0
7	15.72647769647944	0.0	0.0	35.0	0.0	37.0
8	25.147888688642134	17.0	17.0	35.0	17.0	37.0
9	32.83377904385404	32.0	32.0	37.0	27.0	39.0
10	35.59277422962674	37.0	35.0	37.0	32.0	39.0
11	36.518849956121045	37.0	35.0	39.0	32.0	39.0
12	36.19688085687155	37.0	35.0	39.0	32.0	39.0
13	35.68058335478834	37.0	35.0	39.0	31.0	39.0
14	36.70352775724557	39.0	35.0	40.0	31.0	41.0
15	37.17227680561208	39.0	35.0	41.0	32.0	41.0
16	37.31037508893448	39.0	35.0	41.0	32.0	41.0
17	37.28130424310879	39.0	35.0	41.0	32.0	41.0
18	37.15567062823036	39.0	35.0	40.0	32.0	41.0
19	37.0478875812868	39.0	35.0	40.0	32.0	41.0
20	37.1050257321695	39.0	35.0	40.0	32.0	41.0
21	36.93607791352108	39.0	35.0	40.0	32.0	41.0
22	36.963334080798184	39.0	35.0	40.0	32.0	41.0
23	36.96493005666891	38.0	35.0	40.0	32.0	41.0
24	36.7752207097594	38.0	35.0	40.0	32.0	41.0
25	36.519882564967155	38.0	35.0	40.0	31.0	41.0
26	36.37463630298349	38.0	35.0	40.0	31.0	41.0
27	36.24124981659428	38.0	35.0	40.0	31.0	41.0
28	36.195831637695484	38.0	35.0	40.0	31.0	41.0
29	36.26283909296525	38.0	35.0	40.0	31.0	41.0
30	36.01813017515593	38.0	35.0	40.0	30.0	41.0
31	35.83463586004136	38.0	34.0	40.0	30.0	41.0
32	35.62504117977637	38.0	34.0	40.0	29.0	41.0
33	35.34053806395531	38.0	34.0	40.0	27.0	41.0
34	35.117891816920945	38.0	34.0	40.0	26.0	41.0
35	34.88668709737252	38.0	34.0	40.0	24.0	41.0
36	34.64390497783905	38.0	34.0	40.0	23.0	41.0
37	34.47024536225746	37.0	33.0	40.0	23.0	41.0
38	34.43906500452631	37.0	33.0	40.0	23.0	41.0
39	34.35476481156965	37.0	33.0	40.0	23.0	41.0
40	34.15250359198385	37.0	33.0	40.0	23.0	41.0
41	34.05722258672669	37.0	33.0	40.0	22.0	41.0
42	34.03334246901481	37.0	33.0	40.0	22.0	41.0
43	33.91239988815711	37.0	33.0	40.0	21.0	41.0
44	33.69509663059457	36.0	33.0	40.0	20.0	41.0
45	33.606737980349976	36.0	33.0	40.0	20.0	41.0
46	33.59349539478602	36.0	33.0	40.0	20.0	41.0
47	33.57139811915697	36.0	33.0	40.0	20.0	41.0
48	33.57169572090216	36.0	33.0	40.0	20.0	41.0
49	33.51788655698312	36.0	33.0	40.0	20.0	41.0
50	33.348906901868936	36.0	32.0	40.0	19.0	41.0
51	33.14876349935358	35.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	14.0
15	32.0
16	95.0
17	281.0
18	580.0
19	1057.0
20	1856.0
21	2895.0
22	4637.0
23	6869.0
24	9889.0
25	13245.0
26	15424.0
27	15954.0
28	16121.0
29	17943.0
30	21410.0
31	27226.0
32	36809.0
33	56661.0
34	72133.0
35	71092.0
36	77411.0
37	119525.0
38	112014.0
39	21264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.707517004825302	23.635392183732396	28.489345857522125	17.167744953920177
2	32.35955273918183	24.37275241472672	30.48023232314843	12.787462522943018
3	27.50186174115015	23.41309060104479	35.70113586973072	13.383911788074338
4	24.2844408270837	27.434174646546023	33.474936396278174	14.806448130092104
5	20.98770558743816	35.34262958133663	30.86226991232514	12.807394918900064
6	20.971095257473955	38.10382009905293	30.550549386663565	10.374535256809542
7	37.607309652539584	55.82192065245376	5.7701517907319895	0.8006179042746684
8	85.70141270856345	3.5911533382610648	9.076991647772417	1.6304423054030637
9	81.76725605654156	4.812704687711955	10.795468701985765	2.624570553760717
10	55.151970677230835	20.87946160383809	15.280673050570147	8.68789466836092
11	51.298512544951706	17.81568624194053	20.732044925405777	10.153756287701988
12	44.57465097544163	20.627815104880394	23.658369806849546	11.139164112828434
13	20.10486654984068	43.932384883492375	23.97728814216228	11.985460424504666
14	13.222237909756076	45.28197419308401	29.25397471354102	12.241813183618893
15	11.168647448514898	22.269469383009294	54.57822219638393	11.983660972091876
16	12.891277085219297	16.754286157227845	52.39867006624753	17.95576669130532
17	14.214289866868205	17.003302687274548	31.70385996384485	37.0785474820124
18	20.76429664941961	22.223375717358625	36.96227517226296	20.050052460958803
19	28.804526868592912	22.566102192286717	28.514676610717537	20.114694328402834
20	32.143895288479904	20.503237630148856	27.45812120557775	19.89474587579349
21	21.06785042951545	26.88880214605463	30.165466570326753	21.877880854103164
22	21.571973943929063	23.930917637678874	27.913659504846063	26.583448913545997
23	17.912164575149287	29.694148457592444	27.106397468585712	25.28728949867256
24	19.248880879018664	21.92286716442289	43.046085360485684	15.782166596072763
25	15.047713172822178	24.372475575893983	39.77551139053377	20.804299860750067
26	14.774057986661903	35.295290140938654	30.49504320069985	19.435608671699597
27	16.23023024685719	35.06980491167457	32.32384052975879	16.376124311709454
28	13.514026039460608	28.264137467090784	42.88012048026001	15.341716013188602
29	13.593478784456053	23.882055583700836	41.86689035244352	20.65757527939959
30	17.124973354262348	30.133906943394763	34.742442991963365	17.99867671037952
31	28.533501651343634	26.229787304724812	27.155536361396486	18.081174682535067
32	30.564114489467663	26.07544965547407	28.049725791136176	15.310710063922087
33	28.487269566276602	27.286481129280965	26.496244681233925	17.730004623208508
34	18.980624050096758	27.3974935012084	30.136398492889395	23.48548395580545
35	19.949975222924472	25.8044244382248	32.421287798882126	21.824312539968606
36	30.719697913465716	24.89736200276285	27.330083245437002	17.052856838334428
37	20.10888071291536	32.46807356161464	29.64016488520878	17.782880840261225
38	19.467860395713426	33.91386436558229	24.337870721801888	22.280404516902394
39	21.376802566849655	31.55893483490716	28.493913698262286	18.5703488999809
40	23.997082118702956	26.29525968866705	25.793489304331697	23.9141688882983
41	16.63247707082368	24.39960578150218	28.14260521951935	30.825311928154786
42	22.7920026797999	25.30652979754776	24.691393911206713	27.210073611445623
43	23.084344487169904	26.597290855182838	25.786568333513276	24.53179632413398
44	18.943942904759137	32.15538410003848	28.33653082185144	20.56414217335094
45	16.60340899338632	38.6957015234441	22.60430595120439	22.096583531965198
46	21.946536884621878	33.408910334670466	25.90270222384634	18.741850556861312
47	21.5346007015096	27.84071247241993	27.68775901733288	22.936927808737586
48	23.659061903931388	23.668612843660807	31.123190512179526	21.549134740228283
49	20.620894134061974	24.111278137206863	32.3434960868831	22.924331641848063
50	18.072315839887494	34.68264580409223	26.86319455402648	20.381843801993792
51	18.208382126177604	35.31840618347217	23.658508226265916	22.814703464084314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	471.0
2	676.0
3	4359.0
4	8042.0
5	6374.0
6	4706.0
7	4973.5
8	5241.0
9	5542.5
10	5844.0
11	5808.0
12	5772.0
13	5614.5
14	5457.0
15	5113.0
16	4769.0
17	4604.5
18	4440.0
19	4267.0
20	4094.0
21	4114.5
22	4135.0
23	4189.5
24	4244.0
25	4720.0
26	5821.5
27	6447.0
28	7638.0
29	8829.0
30	10453.5
31	12078.0
32	13867.5
33	15657.0
34	16559.5
35	17462.0
36	20129.0
37	22796.0
38	33472.5
39	44149.0
40	56452.5
41	68756.0
42	75223.5
43	81691.0
44	77227.5
45	72764.0
46	68294.5
47	63825.0
48	58826.5
49	53828.0
50	49440.0
51	45052.0
52	40282.5
53	35513.0
54	31259.0
55	27005.0
56	24342.5
57	21680.0
58	20538.0
59	19396.0
60	17294.0
61	15192.0
62	12703.5
63	10215.0
64	8202.5
65	6190.0
66	5131.0
67	4072.0
68	3489.5
69	2907.0
70	2404.0
71	1901.0
72	1454.5
73	1008.0
74	804.5
75	464.0
76	327.0
77	217.0
78	107.0
79	83.0
80	59.0
81	40.0
82	21.0
83	18.5
84	16.0
85	14.5
86	13.0
87	7.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.901321530012144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.43214962080624	19.28162854467704
2	5.598299719960057	2.7881012269620467
3	2.323916547997866	1.7360577951183225
4	1.4568260202692398	1.4510757257604934
5	1.119526787602923	1.3938848249780997
6	0.9008927013357567	1.3460051292001725
7	0.7280039818779717	1.2689782859510752
8	0.603169904920985	1.2015782191731441
9	0.5395228798929694	1.2091349434511658
>10	8.225279729954876	49.44818022737645
>50	1.0116569497228354	15.852661272710794
>100	0.05908299541080999	2.025182161976294
>500	0.0	0.0
>1k	0.0016721602474757543	0.9975316426649049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3003	0.41567350735422354	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2327	0.3221019818892036	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1857	0.2570448561960683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009412520313049353	0.0
2	0.0	0.0	0.0	0.044294213237879305	0.0
3	0.0	0.0	0.0	0.057997735458348215	0.0
4	0.0	0.0	0.0	0.09163365363586282	0.0
5	0.0	0.0	0.0	0.15655235991262967	0.0
6	0.0	0.0	0.0	0.2221631632712384	0.0
7	0.0	0.0	0.0	0.25856746977612044	0.0
8	0.0	0.0	0.0	0.5211491026269237	0.0
9	0.0	0.0	0.0	0.6043391718643157	0.0
10	0.0	0.0	0.0	0.7974342576981959	0.0
11	0.0	0.0	0.0	0.976825821311607	0.0
12	0.0	0.0	0.0	1.1448669927828117	0.0
13	0.0	0.0	0.0	1.2133846038851561	0.0
14	0.0	0.0	0.0	1.2362238075859378	0.0
15	0.0	0.0	0.0	1.2831479897348161	0.0
16	0.0	0.0	0.0	1.3911151345021469	0.0
17	0.0	0.0	0.0	1.5201220305574703	0.0
18	0.0	0.0	0.0	1.691900526270621	0.0
19	0.0	0.0	0.0	1.7748137566752764	0.0
20	0.0	0.0	0.0	1.8681084433075597	0.0
21	0.0	0.0	0.0	1.9888101743807807	0.0
22	0.0	0.0	0.0	2.109096647204897	0.0
23	0.0	0.0	0.0	2.2608043275446335	0.0
24	0.0	0.0	0.0	2.359497371415283	0.0
25	0.0	0.0	0.0	2.4390885358270977	0.0
26	0.0	0.0	0.0	2.5274001234701196	0.0
27	0.0	0.0	0.0	2.610867031540248	0.0
28	0.0	0.0	0.0	2.699455458016007	0.0
29	0.0	0.0	0.0	2.7902585951536594	0.0
30	0.0	0.0	0.0	2.9113755844759854	0.0
31	0.0	0.0	0.0	3.0398288028658356	0.0
32	0.0	0.0	0.0	3.1519485301242174	0.0
33	0.0	0.0	0.0	3.255763092400497	0.0
34	0.0	0.0	0.0	3.372312240982667	0.0
35	0.0	0.0	0.0	3.521943630076878	0.0
36	0.0	0.0	0.0	3.6512273649649383	0.0
37	0.0	0.0	0.0	3.7789884862729464	0.0
38	0.0	0.0	0.0	3.8995517979297993	0.0
39	0.0	0.0	0.0	4.019146173672073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.000004	25
GCGAATG	30	2.1643773E-6	45.000004	1
CATGCCG	25	3.889361E-5	45.000004	37
CGTTAAG	25	3.889361E-5	45.000004	2
AACATCG	25	3.889361E-5	45.000004	19
GGAACGT	35	1.2110831E-7	45.000004	8
AACGACC	25	3.889361E-5	45.000004	29
ATGCGGC	25	3.889361E-5	45.000004	19
AATAGGC	60	0.0	45.000004	4
CCCGGTT	25	3.889361E-5	45.000004	44
TCCGACA	20	7.031546E-4	45.0	23
TAGGTTC	20	7.031546E-4	45.0	32
CGCATGA	20	7.031546E-4	45.0	38
CGTTGGC	20	7.031546E-4	45.0	30
CGGCAAC	20	7.031546E-4	45.0	8
CGACACA	20	7.031546E-4	45.0	25
TTATCGC	40	6.8084773E-9	45.0	31
CATTATC	20	7.031546E-4	45.0	17
ACGATGT	20	7.031546E-4	45.0	45
TCGTGAA	20	7.031546E-4	45.0	1
>>END_MODULE