FastQCFastQC Report
Thu 26 May 2016
SRR1546421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546421_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1405008
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49900.35515812009611336No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42210.30042533565645174No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.15081764658991265No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.1446255110291187No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15120.10761504560828122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG254.443347E-544.021
CAACGTT254.443347E-544.035
ACGTTAT207.8570814E-444.038
CGTGAAT302.5281206E-644.032
CGCATCG207.8570814E-444.021
ATTACGG502.7284841E-1144.02
ACTCTAT207.8570814E-444.019
ATTAACG302.5281206E-644.01
TTCGTCA207.8570814E-444.01
TACGCTA207.8570814E-444.039
GCCGACC302.5281206E-644.014
CGCTAAT207.8570814E-444.032
ACTACGG454.802132E-1044.02
ACGTCAA207.8570814E-444.01
CGTAAGC207.8570814E-444.031
CGTAAAT207.8570814E-444.023
CGGTCGA351.4463876E-744.029
ACGAGTT207.8570814E-444.010
TAGTCCG207.8570814E-444.012
TACGTCA207.8570814E-444.024
Produced by FastQC (version 0.11.5)