FastQCFastQC Report
Thu 26 May 2016
SRR1546419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546419_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences666767
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.29680533079771493No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18290.274308716538161No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.12193164928678234No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.11968198786082695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.8540994E-444.00000425
GCGAAGT207.8540994E-444.00000443
CGTGAAT207.8540994E-444.00000421
TTCCGTA207.8540994E-444.00000412
ACTTACG207.8540994E-444.0000041
TACGAAC207.8540994E-444.00000412
CGAATGC207.8540994E-444.00000417
CGCAGCT207.8540994E-444.00000431
TCCGTGC207.8540994E-444.00000415
CATGTAG207.8540994E-444.0000042
CGTTTAG408.303687E-944.0000042
GGTTAGT207.8540994E-444.0000043
ATACCGC207.8540994E-444.00000427
TGCACGT207.8540994E-444.00000438
TAATCCG207.8540994E-444.00000424
CGGAAAT302.5261106E-644.039
TCGTTAC254.440824E-544.039
GCGAATG351.4448597E-744.01
TTCGTTA254.440824E-544.013
TTCGTAA302.5261106E-644.01
Produced by FastQC (version 0.11.5)