##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546419_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	666767
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54884689854177	31.0	31.0	33.0	30.0	34.0
2	31.733680581072548	31.0	31.0	34.0	30.0	34.0
3	31.798769285222573	31.0	31.0	34.0	30.0	34.0
4	35.70041258790552	37.0	35.0	37.0	35.0	37.0
5	27.055733112166617	35.0	25.0	37.0	0.0	37.0
6	31.316441875497738	35.0	26.0	37.0	17.0	37.0
7	14.996797981903724	0.0	0.0	32.0	0.0	37.0
8	25.10298200120882	17.0	17.0	35.0	17.0	37.0
9	33.256200441833506	32.0	32.0	37.0	28.0	39.0
10	36.10610603104233	37.0	35.0	39.0	32.0	39.0
11	37.07843369572879	39.0	37.0	39.0	34.0	39.0
12	36.959165645570344	39.0	35.0	39.0	33.0	39.0
13	36.39787811934304	39.0	35.0	39.0	32.0	39.0
14	37.71549581787941	40.0	36.0	41.0	33.0	41.0
15	38.017250403814224	40.0	36.0	41.0	33.0	41.0
16	38.10706438680978	40.0	36.0	41.0	34.0	41.0
17	38.149493001303306	40.0	36.0	41.0	34.0	41.0
18	38.119352037518354	40.0	36.0	41.0	34.0	41.0
19	38.03193619360286	40.0	36.0	41.0	34.0	41.0
20	38.04927808364841	40.0	36.0	41.0	34.0	41.0
21	37.91857725412326	40.0	35.0	41.0	34.0	41.0
22	37.839539149358025	40.0	35.0	41.0	33.0	41.0
23	37.74514935502207	40.0	35.0	41.0	33.0	41.0
24	37.620735579295314	39.0	35.0	41.0	33.0	41.0
25	37.43962433653735	39.0	35.0	41.0	33.0	41.0
26	37.197320803219114	39.0	35.0	40.0	33.0	41.0
27	36.96723593099239	39.0	35.0	40.0	32.0	41.0
28	37.00708943304033	39.0	35.0	40.0	32.0	41.0
29	37.0231940093016	39.0	35.0	40.0	33.0	41.0
30	36.844992328654534	38.0	35.0	40.0	32.0	41.0
31	36.64978770695011	38.0	35.0	40.0	32.0	41.0
32	36.40981482286916	38.0	35.0	40.0	31.0	41.0
33	36.22485515929853	38.0	35.0	40.0	31.0	41.0
34	36.01853271082702	38.0	35.0	40.0	30.0	41.0
35	35.887959362116	38.0	35.0	40.0	30.0	41.0
36	35.66978419747828	38.0	35.0	40.0	29.0	41.0
37	35.47805155324124	38.0	35.0	40.0	29.0	41.0
38	35.32586345754964	38.0	34.0	40.0	29.0	41.0
39	35.28761771353411	38.0	34.0	40.0	28.0	41.0
40	35.07277954667823	38.0	34.0	40.0	27.0	41.0
41	34.91374048205745	37.0	34.0	40.0	27.0	40.0
42	34.924570352162	37.0	34.0	40.0	27.0	41.0
43	34.78476289318458	37.0	34.0	40.0	26.0	41.0
44	34.574331063174995	37.0	34.0	40.0	26.0	40.0
45	34.393299308454075	37.0	34.0	40.0	26.0	40.0
46	34.35710825520759	36.0	34.0	40.0	26.0	40.0
47	34.20355986424043	36.0	33.0	39.0	25.0	40.0
48	34.176127492812334	36.0	33.0	39.0	25.0	40.0
49	34.05475375955918	36.0	33.0	39.0	24.0	40.0
50	34.14515565407406	36.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	2.0
15	5.0
16	25.0
17	85.0
18	225.0
19	446.0
20	823.0
21	1331.0
22	2289.0
23	3443.0
24	5227.0
25	7977.0
26	9973.0
27	10806.0
28	11507.0
29	13175.0
30	15964.0
31	21648.0
32	30540.0
33	58685.0
34	57936.0
35	69513.0
36	87066.0
37	165268.0
38	74968.0
39	17835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.842558194991653	23.44717120073429	30.256896337101267	15.453374267172789
2	30.404924058929133	25.435421968993666	31.300439283887776	12.859214688189427
3	28.3185880524981	22.539357826647088	35.06502271408153	14.07703140677328
4	26.279644913440524	26.937595891818283	32.1968543734168	14.585904821324391
5	17.366936276090446	47.82420245753014	24.191659155297128	10.617202111082282
6	20.925150764809896	39.67097951758261	28.58224837162007	10.821621345987428
7	39.173204432732874	55.909485622413825	3.8655182395049543	1.051791705348345
8	87.96326152913987	3.1531254546190794	7.26550654126554	1.6181064749755163
9	84.56177345309531	4.774981365304522	8.369790346552843	2.293454835047325
10	52.95792983156035	23.595048945133755	13.519415327993137	9.927605895312755
11	48.466255828497815	19.2202073587925	19.74062903533018	12.572907777379505
12	42.56674370507238	21.125820564005117	23.42842402218466	12.879011708737833
13	21.629894700847522	41.390770689011305	23.20420776673111	13.775126843410066
14	15.538711423930698	41.815206811374885	27.613694139032074	15.032387625662338
15	13.741381922020734	23.0734274491689	49.27313439327381	13.912056235536552
16	15.34149110558861	17.86726097722293	47.285783489584816	19.505464427603645
17	16.738980783392098	18.275499537319632	29.625941295834977	35.35957838345329
18	22.150166399956806	22.4177261322171	34.04487624612496	21.387231221701132
19	29.51030869854087	23.489314858113854	25.702081836683575	21.298294606661695
20	31.993035048225245	21.667689012803574	26.035031727724977	20.304244211246207
21	22.861059410558713	26.981089346053416	27.961641772913175	22.19620947047469
22	23.64589129336035	24.849310178818087	25.333137362826896	26.17166116499467
23	20.003839422166962	29.679483237772715	26.152464054159847	24.16421328590047
24	20.827215504066636	22.32593994603812	39.45366222383531	17.39318232605993
25	17.615898807229513	24.796068191737145	36.02712791724845	21.56090508378489
26	17.051983676456693	34.504407086733444	27.53615580855081	20.907453428259046
27	19.196960857390962	32.104318300095834	30.360980672408804	18.3377401701044
28	15.695437836605592	27.79861630824561	38.83215576055804	17.673790094590764
29	16.507115679090298	24.00073788894771	37.524802517221154	21.96734391474083
30	18.960146497952056	29.958291277162786	31.454766057708316	19.626796167176842
31	28.959141649181795	26.759422706882614	24.84256119454022	19.438874449395367
32	29.923046581489487	26.825112820520513	25.859558136500453	17.392282461489547
33	26.91689900669949	27.893552020420927	25.682134838706776	19.507414134172805
34	19.72563129249048	27.85920718931801	29.550202694494477	22.864958823697034
35	21.584601517471622	25.492863324069724	30.671433949190646	22.251101209268004
36	28.62724160013918	25.69503289754892	25.87650558590932	19.801219916402584
37	21.772673212681493	31.921795769736654	28.15616249754412	18.149368520037733
38	20.94614760478548	32.042827554453055	24.47496651753911	22.536058323222356
39	21.991190325855957	30.3056390013303	26.904150925285748	20.799019747527996
40	24.25509960750907	27.12641747417014	24.50016272550981	24.118320192810984
41	17.862311722085828	24.62134448765461	27.486363302323003	30.029980487936562
42	22.137868250828248	26.442370423251297	24.015735631787415	27.404025694133033
43	23.062479096895917	27.998086288013653	25.267147294332204	23.672287320758226
44	20.67078904624854	31.450416712284802	26.617394082190632	21.26140015927603
45	17.71638368425552	36.537801060940325	22.476367306720338	23.269447948083812
46	21.36428467515639	32.66643370172789	25.07802575712355	20.891255865992168
47	22.661739408219063	27.364581630464613	27.49446208345644	22.47921687785988
48	23.707532016431525	23.412526414774575	30.65103701892865	22.228904549865245
49	20.08182768493342	25.081925170261876	31.326685333857256	23.509561810947453
50	19.78217278299616	31.657385563472694	26.865756703616107	21.69468494991504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	434.5
2	694.0
3	3510.5
4	6327.0
5	4888.0
6	3449.0
7	3604.0
8	3759.0
9	3888.0
10	4017.0
11	3991.0
12	3965.0
13	3789.5
14	3614.0
15	3371.5
16	3129.0
17	3035.0
18	2941.0
19	2848.0
20	2755.0
21	2715.5
22	2676.0
23	3023.5
24	3371.0
25	4366.0
26	5361.0
27	6249.0
28	7137.0
29	8663.5
30	10190.0
31	11687.0
32	13184.0
33	15012.5
34	16841.0
35	17630.5
36	18420.0
37	21881.0
38	25342.0
39	34080.5
40	42819.0
41	52904.5
42	62990.0
43	65851.0
44	68712.0
45	65069.5
46	61427.0
47	57456.5
48	53486.0
49	50452.5
50	47419.0
51	43349.0
52	39279.0
53	36445.5
54	33612.0
55	30179.5
56	26747.0
57	25041.5
58	23336.0
59	22439.0
60	21542.0
61	18841.5
62	16141.0
63	13753.5
64	11366.0
65	9395.0
66	7424.0
67	6105.0
68	4786.0
69	4103.5
70	3421.0
71	2844.5
72	2268.0
73	1797.0
74	1326.0
75	939.5
76	553.0
77	440.0
78	327.0
79	283.0
80	239.0
81	176.5
82	114.0
83	73.0
84	32.0
85	30.5
86	29.0
87	25.5
88	22.0
89	12.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	666767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.635253542838228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88495090570814	15.818635825795058
2	6.019910521937846	2.725244019385255
3	2.977460852746595	2.021867439473835
4	2.0481718608731576	1.854435574806828
5	1.6794975776845282	1.9007926747735973
6	1.466140302175135	1.991187448146458
7	1.3148941464476414	2.083407367078536
8	1.1547063072047727	2.090965602487558
9	1.0042299024442092	2.0457898611411935
>10	11.767673632156162	56.45178502361268
>50	0.6471975609763599	9.04792964127559
>100	0.03246131967279549	1.1384345803740092
>500	0.0013525549863664785	0.24660724875386966
>1k	0.0013525549863664785	0.5829176928955528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1979	0.29680533079771493	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1829	0.274308716538161	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.12193164928678234	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	798	0.11968198786082695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007948803705042392	0.0
2	0.0	0.0	0.0	0.03044541796459633	0.0
3	0.0	0.0	0.0	0.042143657379564374	0.0
4	0.0	0.0	0.0	0.05879115193163429	0.0
5	0.0	0.0	0.0	0.09613553160249383	0.0
6	0.0	0.0	0.0	0.1576262772452746	0.0
7	0.0	0.0	0.0	0.20186961862239733	0.0
8	0.0	0.0	0.0	0.39294086240020876	0.0
9	0.0	0.0	0.0	0.46717968945673677	0.0
10	0.0	0.0	0.0	0.6090583367203236	0.0
11	0.0	0.0	0.0	0.7638350428260546	0.0
12	0.0	0.0	0.0	0.8925156763907032	0.0
13	0.0	0.0	0.0	0.9435080020456921	0.0
14	0.0	0.0	0.0	0.9610553611681442	0.0
15	0.0	0.0	0.0	0.99405039541549	0.0
16	0.0	0.0	0.0	1.0754881390350752	0.0
17	0.0	0.0	0.0	1.1813722034833758	0.0
18	0.0	0.0	0.0	1.325950444458109	0.0
19	0.0	0.0	0.0	1.3932903098083738	0.0
20	0.0	0.0	0.0	1.4666292722945198	0.0
21	0.0	0.0	0.0	1.5560158196191474	0.0
22	0.0	0.0	0.0	1.652301328650038	0.0
23	0.0	0.0	0.0	1.77123342936888	0.0
24	0.0	0.0	0.0	1.8573204732687731	0.0
25	0.0	0.0	0.0	1.920310993195524	0.0
26	0.0	0.0	0.0	1.9970994365348016	0.0
27	0.0	0.0	0.0	2.063539437314684	0.0
28	0.0	0.0	0.0	2.143477406650299	0.0
29	0.0	0.0	0.0	2.2217656242735466	0.0
30	0.0	0.0	0.0	2.3239002530119217	0.0
31	0.0	0.0	0.0	2.424085175181135	0.0
32	0.0	0.0	0.0	2.5118219707933958	0.0
33	0.0	0.0	0.0	2.595659353267333	0.0
34	0.0	0.0	0.0	2.694794433437768	0.0
35	0.0	0.0	0.0	2.820925450719667	0.0
36	0.0	0.0	0.0	2.924259898885218	0.0
37	0.0	0.0	0.0	3.0314937601890914	0.0
38	0.0	0.0	0.0	3.130328885502732	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.8540994E-4	44.000004	25
GCGAAGT	20	7.8540994E-4	44.000004	43
CGTGAAT	20	7.8540994E-4	44.000004	21
TTCCGTA	20	7.8540994E-4	44.000004	12
ACTTACG	20	7.8540994E-4	44.000004	1
TACGAAC	20	7.8540994E-4	44.000004	12
CGAATGC	20	7.8540994E-4	44.000004	17
CGCAGCT	20	7.8540994E-4	44.000004	31
TCCGTGC	20	7.8540994E-4	44.000004	15
CATGTAG	20	7.8540994E-4	44.000004	2
CGTTTAG	40	8.303687E-9	44.000004	2
GGTTAGT	20	7.8540994E-4	44.000004	3
ATACCGC	20	7.8540994E-4	44.000004	27
TGCACGT	20	7.8540994E-4	44.000004	38
TAATCCG	20	7.8540994E-4	44.000004	24
CGGAAAT	30	2.5261106E-6	44.0	39
TCGTTAC	25	4.440824E-5	44.0	39
GCGAATG	35	1.4448597E-7	44.0	1
TTCGTTA	25	4.440824E-5	44.0	13
TTCGTAA	30	2.5261106E-6	44.0	1
>>END_MODULE