FastQCFastQC Report
Thu 26 May 2016
SRR1546418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546418_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences255847
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6790.26539298877844963No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.21106364350568893No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.18839384475878163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG207.022994E-445.01
ACTATGC207.022994E-445.08
CGGAAAG207.022994E-445.02
ACACGTG207.022994E-445.042
CGTGCGG253.8822775E-545.02
AGCCATT207.022994E-445.040
GATCATG207.022994E-445.010
GTCTAAT207.022994E-445.019
CGATTGT207.022994E-445.012
TGAAACT207.022994E-445.027
CTTATGG253.8822775E-545.02
CATAGGT207.022994E-445.04
CTACGGG207.022994E-445.03
AGCATAT406.7793735E-945.031
CGCTAGG207.022994E-445.03
CGGGATA207.022994E-445.06
CCTGGTT406.7793735E-945.012
ACTTTGC207.022994E-445.039
CGTTTTA3850.045.01
AATACGA207.022994E-445.027