##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546418_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	255847
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.031432848538383	31.0	31.0	33.0	30.0	33.0
2	31.168925177938377	31.0	31.0	33.0	30.0	34.0
3	31.14010326484188	31.0	31.0	34.0	28.0	34.0
4	35.114822530653086	35.0	35.0	37.0	33.0	37.0
5	32.80732234499525	35.0	35.0	37.0	28.0	37.0
6	33.98932956024499	35.0	35.0	37.0	28.0	37.0
7	15.257298307191407	0.0	0.0	35.0	0.0	37.0
8	24.890813650345713	17.0	17.0	35.0	17.0	37.0
9	32.73617435420388	32.0	32.0	37.0	27.0	39.0
10	35.53884157328403	37.0	35.0	37.0	32.0	39.0
11	36.508643837918754	37.0	35.0	39.0	32.0	39.0
12	36.280949161022015	37.0	35.0	39.0	32.0	39.0
13	35.865599362118765	37.0	35.0	39.0	31.0	39.0
14	36.88908605533776	39.0	35.0	40.0	31.0	41.0
15	37.28321223231072	39.0	35.0	41.0	32.0	41.0
16	37.38490973120654	39.0	35.0	41.0	32.0	41.0
17	37.37196058581887	39.0	35.0	41.0	32.0	41.0
18	37.275043287589845	39.0	35.0	41.0	32.0	41.0
19	37.17891552373098	39.0	35.0	40.0	32.0	41.0
20	37.270630494006184	39.0	35.0	40.0	32.0	41.0
21	37.139993824434136	39.0	35.0	40.0	32.0	41.0
22	37.18553275981348	39.0	35.0	41.0	32.0	41.0
23	37.170082119391665	39.0	35.0	41.0	32.0	41.0
24	37.02763761154127	39.0	35.0	41.0	32.0	41.0
25	36.763557907655745	38.0	35.0	40.0	31.0	41.0
26	36.63222550977733	38.0	35.0	40.0	31.0	41.0
27	36.518403577137896	38.0	35.0	40.0	31.0	41.0
28	36.45660101545064	38.0	35.0	40.0	31.0	41.0
29	36.534260710502764	38.0	35.0	40.0	31.0	41.0
30	36.28274711057781	38.0	35.0	40.0	30.0	41.0
31	36.15490507998921	38.0	35.0	40.0	30.0	41.0
32	36.05025269008431	38.0	35.0	40.0	30.0	41.0
33	35.878591501952336	38.0	34.0	40.0	30.0	41.0
34	35.723819313886814	38.0	34.0	40.0	29.0	41.0
35	35.56577954793294	38.0	34.0	40.0	28.0	41.0
36	35.345436921284985	38.0	34.0	40.0	27.0	41.0
37	35.16628297380856	38.0	34.0	40.0	26.0	41.0
38	35.11665956606878	38.0	34.0	40.0	26.0	41.0
39	35.04019589832986	38.0	34.0	40.0	26.0	41.0
40	34.89965487185701	38.0	34.0	40.0	25.0	41.0
41	34.771078027102135	38.0	34.0	40.0	24.0	41.0
42	34.750389881452584	38.0	34.0	40.0	24.0	41.0
43	34.66083245064433	37.0	33.0	40.0	24.0	41.0
44	34.3421849777406	37.0	33.0	40.0	23.0	41.0
45	34.29567280444954	37.0	33.0	40.0	23.0	41.0
46	34.302278314774064	37.0	33.0	40.0	23.0	41.0
47	34.27815843062455	37.0	33.0	40.0	23.0	41.0
48	34.28103905849981	37.0	33.0	40.0	23.0	41.0
49	34.20931259698179	36.0	33.0	40.0	24.0	41.0
50	34.091730604619165	36.0	33.0	40.0	24.0	41.0
51	33.909789835331274	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	13.0
16	21.0
17	100.0
18	145.0
19	296.0
20	522.0
21	771.0
22	1232.0
23	1879.0
24	2695.0
25	3437.0
26	4296.0
27	4629.0
28	5083.0
29	5926.0
30	7416.0
31	9613.0
32	12869.0
33	19641.0
34	25238.0
35	26109.0
36	29281.0
37	44609.0
38	41935.0
39	8083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.27082592330573	24.178512939373924	30.59015739875785	14.9605037385625
2	29.858860959870547	25.35030701942958	31.99099461787709	12.799837402822781
3	29.063463710733366	22.209758175784746	34.60544778715404	14.121330326327843
4	26.76130656212502	27.67122538079399	30.898544833435608	14.668923223645383
5	21.188053797777577	36.30060153138399	29.28429881921617	13.22704585162226
6	20.914061919819265	40.24827338213854	28.185986155788424	10.65167854225377
7	38.09542421838052	57.0434673848042	4.099715845798466	0.7613925510168186
8	88.53299042005574	3.273440767333602	6.662184821397163	1.5313839912134986
9	84.97109600659769	4.892377084742053	7.807791375314152	2.3287355333461015
10	52.97619280272976	24.178512939373924	13.018718218310163	9.82657603958616
11	48.76430053899401	19.548401974617644	19.212654438003963	12.474643048384387
12	42.729834627726724	21.577348962465848	22.99733825294023	12.69547815686719
13	21.793102909160552	42.01495425000098	22.455608234608963	13.736334606229505
14	15.28960667899174	42.38978764652312	27.308508600843474	15.01209707364167
15	13.712883090284429	23.435881601113167	49.08363201444613	13.767603294156272
16	15.246612233092435	18.172970564438902	46.93586401247621	19.644553189992457
17	16.535663892873476	18.76043103886307	29.300714880377726	35.40319018788573
18	22.071394231708794	22.574429248730688	33.93668872412027	21.417487795440245
19	29.980808842784946	23.489038370588673	25.302231411742177	21.22792137488421
20	32.4885576145118	21.91935023666488	25.65478586811649	19.937306280706828
21	22.713184051405726	27.745488514620064	27.477359515648025	22.06396791832619
22	23.318233162788697	25.249465500865753	24.70578118953906	26.726520146806486
23	19.85522597489906	30.166466677350133	25.828327086110058	24.149980261640746
24	20.382885083663282	22.679961070483532	39.87226740981915	17.064886436034037
25	17.429166650380893	25.162304033269884	36.20992233639636	21.198606979952864
26	16.786204254886712	35.32228245787522	27.123632483476452	20.767880803761624
27	19.051620695181104	33.40824789815788	29.529367160842224	18.010764245818788
28	15.514741232064477	28.42401904263095	38.447587816155746	17.613651909148828
29	16.376975301645125	24.335638096205937	37.352402021520675	21.934984580628267
30	19.024651451844264	30.766043768345924	30.816073669028754	19.393231110781052
31	29.324166396322802	27.022009247714458	24.58696017541734	19.066864180545405
32	30.594847701946865	26.535781150453204	25.77243430644096	17.096936841158975
33	27.709920382103366	27.849847760575656	25.130644486744032	19.309587370576946
34	19.877504915046885	27.85141119497199	28.936043807431783	23.335040082549334
35	21.775514272201747	25.21702423714173	30.804738769655305	22.202722721001223
36	29.092778105664713	26.089811488897663	25.514467631045118	19.30294277439251
37	21.630505731941355	32.9075580327305	27.706402654711603	17.75553358061654
38	20.691663376940124	32.87120818301563	24.256684659190846	22.180443780853402
39	21.96312639976236	30.946620441123013	26.65929246776394	20.43096069135069
40	24.717116088912512	27.01223778273734	24.282872185329513	23.98777394302063
41	17.76061474240464	24.80584099090472	27.285838802096567	30.147705464594072
42	21.639886338319386	26.36380336685597	23.91820111238357	28.078109182441068
43	22.73311783995904	27.66770765340223	25.333109241069856	24.266065265568876
44	20.066680477003835	31.770159509394286	26.912177981371677	21.2509820322302
45	17.567139735857758	37.30549898963052	22.34225924087443	22.78510203363729
46	21.01803030717577	33.39495870578901	24.738613311862167	20.848397675173054
47	22.357502726238728	27.498856738597677	27.219392840252183	22.92424769491141
48	23.22520881620656	23.815796159423407	31.09319241577974	21.86580260859029
49	20.090522851547995	25.501569297275324	31.61068920096777	22.797218650208915
50	19.893530117609355	32.6054243356381	26.351686750284347	21.149358796468203
51	18.325796276680986	34.20286343009689	24.36729764273179	23.104042650490335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	171.0
2	264.0
3	1150.0
4	2036.0
5	1612.0
6	1188.0
7	1177.5
8	1167.0
9	1266.5
10	1366.0
11	1321.0
12	1276.0
13	1233.5
14	1191.0
15	1165.0
16	1139.0
17	1100.0
18	1061.0
19	1035.5
20	1010.0
21	972.5
22	935.0
23	1040.5
24	1146.0
25	1497.5
26	2173.5
27	2498.0
28	3095.0
29	3692.0
30	4217.5
31	4743.0
32	5508.5
33	6274.0
34	6823.0
35	7372.0
36	8022.0
37	8672.0
38	11709.0
39	14746.0
40	18507.5
41	22269.0
42	24221.0
43	26173.0
44	24864.5
45	23556.0
46	22294.0
47	21032.0
48	19886.0
49	18740.0
50	17297.0
51	15854.0
52	14603.0
53	13352.0
54	12205.5
55	11059.0
56	10333.0
57	9607.0
58	8995.0
59	8383.0
60	7724.5
61	7066.0
62	6052.5
63	5039.0
64	4234.0
65	3429.0
66	2893.5
67	2358.0
68	1962.0
69	1566.0
70	1325.5
71	1085.0
72	884.0
73	683.0
74	553.5
75	319.5
76	215.0
77	153.5
78	92.0
79	92.0
80	92.0
81	67.5
82	43.0
83	24.5
84	6.0
85	9.0
86	12.0
87	9.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	255847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.82644314766247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.83074410207917	20.95158434533139
2	7.970329251968021	5.073344616118227
3	4.602895845358419	4.394814088107346
4	3.325678214842743	4.233780345284487
5	2.5040834121360236	3.98480341766759
6	2.1405676249892545	4.087599229226843
7	1.820035123484839	4.054767106903736
8	1.5793287238876539	4.021153267382458
9	1.4737126505950116	4.221272870113779
>10	8.718238422144989	43.4568316220241
>50	0.022105689758925174	0.43619819657842385
>100	0.009824751003966743	0.6073942629774827
>500	0.002456187750991686	0.4764566322841386
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	679	0.26539298877844963	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.21106364350568893	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.18839384475878163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010553182175284448	0.0
2	0.0	0.0	0.0	0.04182187010205318	0.0
3	0.0	0.0	0.0	0.050420759281914584	0.0
4	0.0	0.0	0.0	0.06918197203797583	0.0
5	0.0	0.0	0.0	0.10983126634277518	0.0
6	0.0	0.0	0.0	0.15556172243567445	0.0
7	0.0	0.0	0.0	0.18487611736702014	0.0
8	0.0	0.0	0.0	0.3482550117843868	0.0
9	0.0	0.0	0.0	0.40688380164707816	0.0
10	0.0	0.0	0.0	0.5268773915660532	0.0
11	0.0	0.0	0.0	0.6523430018722127	0.0
12	0.0	0.0	0.0	0.7735091675884416	0.0
13	0.0	0.0	0.0	0.818067047884087	0.0
14	0.0	0.0	0.0	0.834092250446556	0.0
15	0.0	0.0	0.0	0.8684878071660016	0.0
16	0.0	0.0	0.0	0.9376697792039774	0.0
17	0.0	0.0	0.0	1.0396838735650604	0.0
18	0.0	0.0	0.0	1.1706215042584045	0.0
19	0.0	0.0	0.0	1.2249508495311652	0.0
20	0.0	0.0	0.0	1.2886608011819565	0.0
21	0.0	0.0	0.0	1.380121713367755	0.0
22	0.0	0.0	0.0	1.4739277771480612	0.0
23	0.0	0.0	0.0	1.5810230332972441	0.0
24	0.0	0.0	0.0	1.6552861671233199	0.0
25	0.0	0.0	0.0	1.7244681391612957	0.0
26	0.0	0.0	0.0	1.7885689494111716	0.0
27	0.0	0.0	0.0	1.8425074360848477	0.0
28	0.0	0.0	0.0	1.9046539533393005	0.0
29	0.0	0.0	0.0	1.9800896629626301	0.0
30	0.0	0.0	0.0	2.062169968770398	0.0
31	0.0	0.0	0.0	2.1485497191680967	0.0
32	0.0	0.0	0.0	2.228284873381357	0.0
33	0.0	0.0	0.0	2.317400633972648	0.0
34	0.0	0.0	0.0	2.4237141729236615	0.0
35	0.0	0.0	0.0	2.5386266010545366	0.0
36	0.0	0.0	0.0	2.627351503046743	0.0
37	0.0	0.0	0.0	2.7227210012233876	0.0
38	0.0	0.0	0.0	2.8008927210403094	0.0
39	0.0	0.0	0.0	2.898216512212377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.022994E-4	45.0	1
ACTATGC	20	7.022994E-4	45.0	8
CGGAAAG	20	7.022994E-4	45.0	2
ACACGTG	20	7.022994E-4	45.0	42
CGTGCGG	25	3.8822775E-5	45.0	2
AGCCATT	20	7.022994E-4	45.0	40
GATCATG	20	7.022994E-4	45.0	10
GTCTAAT	20	7.022994E-4	45.0	19
CGATTGT	20	7.022994E-4	45.0	12
TGAAACT	20	7.022994E-4	45.0	27
CTTATGG	25	3.8822775E-5	45.0	2
CATAGGT	20	7.022994E-4	45.0	4
CTACGGG	20	7.022994E-4	45.0	3
AGCATAT	40	6.7793735E-9	45.0	31
CGCTAGG	20	7.022994E-4	45.0	3
CGGGATA	20	7.022994E-4	45.0	6
CCTGGTT	40	6.7793735E-9	45.0	12
ACTTTGC	20	7.022994E-4	45.0	39
CGTTTTA	385	0.0	45.0	1
AATACGA	20	7.022994E-4	45.0	27
>>END_MODULE