FastQCFastQC Report
Thu 26 May 2016
SRR1546417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546417_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1693109
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.41775219433598193No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA69130.408302123489982No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.17334973708131018No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.1647265474343353No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA254.4437365E-544.018
GTCGCAT254.4437365E-544.016
CGAACCG207.857539E-444.021
ACACGAT207.857539E-444.043
GTCGATA502.7284841E-1144.01
CTCCGTA254.4437365E-544.018
ACGTTCA207.857539E-444.036
GCGAACC207.857539E-444.020
CGGTTTA302.5284298E-644.01
CGCATAC207.857539E-444.026
GACCGAC207.857539E-444.09
CGACCGT302.5284298E-644.022
CGCGCCA302.5284298E-644.014
CCTACGC254.4437365E-544.044
CGACATA254.4437365E-544.015
CGACACT207.857539E-444.035
CCGTCGA351.4466241E-744.018
TCGGCCG454.802132E-1044.023
CGTTACC302.5284298E-644.033
CCCGTAC302.5284298E-644.029
Produced by FastQC (version 0.11.5)