##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546417_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1693109
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.575460882908306	31.0	31.0	33.0	30.0	34.0
2	31.761616056615374	31.0	31.0	34.0	30.0	34.0
3	31.763803157386796	31.0	31.0	34.0	30.0	34.0
4	35.66946310013118	37.0	35.0	37.0	35.0	37.0
5	27.08861685809951	35.0	25.0	37.0	0.0	37.0
6	31.335301507463488	35.0	26.0	37.0	17.0	37.0
7	15.037810324084273	0.0	0.0	32.0	0.0	37.0
8	25.15060636970213	17.0	17.0	35.0	17.0	37.0
9	33.35390456255327	32.0	32.0	37.0	28.0	39.0
10	36.141566195679076	37.0	35.0	39.0	32.0	39.0
11	37.10252499986711	39.0	37.0	39.0	34.0	39.0
12	36.92658417148571	39.0	35.0	39.0	33.0	39.0
13	36.333377827416896	39.0	35.0	39.0	32.0	39.0
14	37.618785323331224	40.0	35.0	41.0	33.0	41.0
15	37.945923150842624	40.0	36.0	41.0	33.0	41.0
16	38.05627812503507	40.0	36.0	41.0	33.0	41.0
17	38.10406595204444	40.0	36.0	41.0	34.0	41.0
18	38.06572169895736	40.0	36.0	41.0	34.0	41.0
19	37.976064151805936	40.0	36.0	41.0	34.0	41.0
20	37.96434252018033	40.0	36.0	41.0	34.0	41.0
21	37.8126765612846	40.0	35.0	41.0	34.0	41.0
22	37.69699529091157	39.0	35.0	41.0	33.0	41.0
23	37.57127332026467	39.0	35.0	41.0	33.0	41.0
24	37.43578529202786	39.0	35.0	41.0	33.0	41.0
25	37.2652398634701	39.0	35.0	41.0	33.0	41.0
26	37.002768870757876	38.0	35.0	40.0	33.0	41.0
27	36.78154448414131	38.0	35.0	40.0	32.0	41.0
28	36.83780548092297	38.0	35.0	40.0	32.0	41.0
29	36.85341876984884	38.0	35.0	40.0	33.0	41.0
30	36.624913103645426	38.0	35.0	40.0	32.0	41.0
31	36.385438267707514	38.0	35.0	40.0	31.0	41.0
32	36.06898906095237	38.0	35.0	40.0	31.0	41.0
33	35.77920913538349	38.0	35.0	40.0	30.0	41.0
34	35.49027735367304	38.0	35.0	40.0	29.0	41.0
35	35.299575514630185	38.0	35.0	40.0	27.0	41.0
36	35.04825619614567	38.0	34.0	40.0	26.0	41.0
37	34.8235465052752	38.0	34.0	40.0	25.0	41.0
38	34.688639656395424	38.0	34.0	40.0	25.0	41.0
39	34.65955292896086	37.0	34.0	40.0	24.0	41.0
40	34.44834679869991	37.0	34.0	40.0	24.0	40.0
41	34.300423067859185	37.0	34.0	40.0	23.0	40.0
42	34.29923235893259	37.0	34.0	40.0	23.0	40.0
43	34.167014645837924	37.0	33.0	40.0	23.0	41.0
44	33.96481266120492	36.0	33.0	40.0	23.0	40.0
45	33.7035749027381	36.0	33.0	39.0	23.0	40.0
46	33.69513067380777	36.0	33.0	39.0	23.0	40.0
47	33.55761324285678	36.0	33.0	39.0	23.0	40.0
48	33.572253765114944	36.0	33.0	39.0	23.0	40.0
49	33.489982629588525	36.0	33.0	39.0	22.0	40.0
50	33.51126418913372	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	3.0
14	10.0
15	25.0
16	82.0
17	232.0
18	586.0
19	1271.0
20	2425.0
21	4147.0
22	7122.0
23	11147.0
24	17680.0
25	26815.0
26	33565.0
27	33913.0
28	32874.0
29	34678.0
30	41648.0
31	56267.0
32	79190.0
33	156851.0
34	148717.0
35	173956.0
36	206260.0
37	396009.0
38	184811.0
39	42821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.82188388343574	23.274106983070787	29.424921844960956	17.479087288532515
2	32.91252955362	24.206533660857037	30.345004367704618	12.535932417818346
3	26.421334952445473	23.3572085435728	37.07552201305409	13.145934490927635
4	24.081143033319176	27.255185578719388	34.44787075138104	14.215800636580397
5	16.905586114065898	47.54513737745177	25.61855143407778	9.930725074404542
6	20.568787951632174	38.00032957122075	30.62838836719904	10.80249410994803
7	38.01869814642767	55.789024805845344	5.251109054408192	0.9411679933187999
8	85.90539652201954	2.941866117302548	9.658090530497446	1.494646830180455
9	82.54069879730129	4.035711817727034	11.222963199652238	2.200626185319433
10	53.94968664155704	21.49920648936365	16.044094030567436	8.507012838511873
11	50.02442252684263	17.990513310129472	21.320777339202614	10.664286823825282
12	43.16367109264672	20.507716868789903	24.888238146510353	11.440373892053023
13	20.538547724924975	42.9265924402977	24.78056640180874	11.75429343296858
14	13.629128425872167	43.753296450494325	29.759218101138202	12.858357022495303
15	11.550526280351708	22.322898289478115	54.5740410097637	11.552534420406484
16	13.177828480032886	16.184191330859385	52.06203499006856	18.575945199039165
17	14.402675787560046	16.436212907733644	32.202533918371465	36.958577386334845
18	20.401403571772402	21.3156388631801	37.273441934335	21.009515630712492
19	28.093170610988423	22.965798421720045	28.641097531228056	20.299933436063476
20	31.441507900554544	21.32674269642415	27.196772328302547	20.034977074718757
21	21.332708053645693	26.70318331542742	30.39674350558647	21.567365125340423
22	21.695118270589788	24.116462673106103	28.413764264438967	25.774654791865142
23	18.113659545841408	29.366567657486907	28.085078987826538	24.434693808845147
24	19.32639895009713	21.6657639880244	42.37919708654316	16.628639975335314
25	15.033468016530534	24.185861630881416	39.99299513498541	20.787675217602647
26	15.17935348521566	35.3783483520553	30.480199443745203	18.96209871898383
27	16.60584167941934	33.22243281442601	32.92068023972467	17.251045266429983
28	13.213975001018834	27.65634108613208	43.25834898993509	15.871334922914002
29	14.081964008223924	23.395186015785164	42.44564289717909	20.077207078811817
30	16.937716354942296	28.841793410819978	35.21515744113344	19.005332793104284
31	28.020641317245378	26.31389945951501	26.884624675670615	18.780834547568997
32	28.986615746534927	27.079709575697724	28.291208658154908	15.642466019612442
33	25.961884320501515	27.808900667352194	28.059681922427913	18.16953308971838
34	18.108934510418408	28.819585744331878	30.264442513742466	22.807037231507245
35	19.99286519651127	25.923670596518004	32.16804115978357	21.915423047187158
36	28.778477936151774	24.03979897336793	28.190742592473374	18.99098049800692
37	20.302650331431703	30.976032848446263	30.177856239616	18.54346058050604
38	19.765472866779398	31.63121807278799	25.931939408508253	22.67136965192436
39	19.788448351523733	30.846271563142125	29.036701122018727	20.328578963315415
40	23.255738407863877	26.495636134472143	26.194887629798195	24.053737827865778
41	16.69538110068519	24.15591671888815	28.49090046771944	30.657801712707215
42	22.22692100744843	25.45134424304637	24.84016091108133	27.481573838423866
43	22.564879166078498	27.378686192088047	26.440412282965838	23.61602235886762
44	20.430580665509428	31.637301555895103	27.998492713700063	19.93362506489541
45	16.61387423963844	37.78953392841217	22.88889847021072	22.70769336173867
46	21.598432233246648	32.8922119013011	26.61293513884812	18.896420726604134
47	21.99941055183098	27.55079560737082	27.99057827936654	22.459215561431662
48	23.270149765904026	22.95504896613272	30.151573230075556	23.623228037887696
49	20.187359467110504	22.878798706994054	33.49920176432823	23.43464006156721
50	18.417479323540302	32.809346592570236	27.50354525314082	21.26962883074864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	593.0
1	1005.0
2	1417.0
3	11296.0
4	21175.0
5	16254.0
6	11333.0
7	12266.5
8	13200.0
9	14278.5
10	15357.0
11	15449.0
12	15541.0
13	14932.5
14	14324.0
15	13446.5
16	12569.0
17	11494.5
18	10420.0
19	10091.5
20	9763.0
21	10144.0
22	10525.0
23	10237.5
24	9950.0
25	11107.0
26	12264.0
27	13805.0
28	15346.0
29	18209.5
30	21073.0
31	24094.0
32	27115.0
33	31771.0
34	36427.0
35	40202.0
36	43977.0
37	55148.5
38	66320.0
39	93195.0
40	120070.0
41	150189.0
42	180308.0
43	188225.5
44	196143.0
45	184393.0
46	172643.0
47	159167.0
48	145691.0
49	136516.0
50	127341.0
51	114899.0
52	102457.0
53	88984.5
54	75512.0
55	65014.5
56	54517.0
57	50243.5
58	45970.0
59	41283.5
60	36597.0
61	31306.0
62	26015.0
63	21112.5
64	16210.0
65	13297.0
66	10384.0
67	8193.0
68	6002.0
69	4730.0
70	3458.0
71	2869.0
72	2280.0
73	1725.5
74	1171.0
75	1017.0
76	863.0
77	623.5
78	384.0
79	314.0
80	244.0
81	170.5
82	97.0
83	70.0
84	43.0
85	29.0
86	15.0
87	9.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1693109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.771610835272106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52638554875698	15.723647462058304
2	6.721066478676607	2.6577262162877306
3	2.22219092250416	1.318088823642797
4	1.1333727595191994	0.8963442053004818
5	0.742685569064911	0.7342045022262011
6	0.5435506685211637	0.6448123372351461
7	0.46549220897109617	0.644247156183937
8	0.3580078163124767	0.5662712976092695
9	0.30598982527456603	0.5444920570393466
>10	5.327067067743204	27.099080039539558
>50	1.820251909093744	25.50384375559988
>100	0.8314102210871558	22.286173310611076
>500	0.001264502237392609	0.14913243321351316
>1k	6.322511186963045E-4	0.3577678322356032
>5k	6.322511186963045E-4	0.8741685712171814
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7073	0.41775219433598193	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6913	0.408302123489982	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2935	0.17334973708131018	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2789	0.1647265474343353	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018900141691999748	0.0
2	0.0	0.0	0.0	0.06886739129022408	0.0
3	0.0	0.0	0.0	0.09172475014898628	0.0
4	0.0	0.0	0.0	0.12444562045326084	0.0
5	0.0	0.0	0.0	0.1944352076564474	0.0
6	0.0	0.0	0.0	0.30263851884314596	0.0
7	0.0	0.0	0.0	0.37245091721797	0.0
8	0.0	0.0	0.0	0.7187369507810779	0.0
9	0.0	0.0	0.0	0.8452497742319012	0.0
10	0.0	0.0	0.0	1.0985116729046978	0.0
11	0.0	0.0	0.0	1.3988467369791313	0.0
12	0.0	0.0	0.0	1.6580739928734654	0.0
13	0.0	0.0	0.0	1.7698210806274137	0.0
14	0.0	0.0	0.0	1.8077394898969883	0.0
15	0.0	0.0	0.0	1.8761934405876999	0.0
16	0.0	0.0	0.0	2.0367855820269103	0.0
17	0.0	0.0	0.0	2.2339376850515826	0.0
18	0.0	0.0	0.0	2.501610941764529	0.0
19	0.0	0.0	0.0	2.6243437368769524	0.0
20	0.0	0.0	0.0	2.755522532807988	0.0
21	0.0	0.0	0.0	2.9259191227499235	0.0
22	0.0	0.0	0.0	3.1031670140552086	0.0
23	0.0	0.0	0.0	3.3081744884706183	0.0
24	0.0	0.0	0.0	3.454827775411979	0.0
25	0.0	0.0	0.0	3.5766155634398022	0.0
26	0.0	0.0	0.0	3.6988758550099257	0.0
27	0.0	0.0	0.0	3.8053072779129993	0.0
28	0.0	0.0	0.0	3.927508506540335	0.0
29	0.0	0.0	0.0	4.058628239528583	0.0
30	0.0	0.0	0.0	4.230265151269056	0.0
31	0.0	0.0	0.0	4.385246313143454	0.0
32	0.0	0.0	0.0	4.523571725151777	0.0
33	0.0	0.0	0.0	4.6533330104559125	0.0
34	0.0	0.0	0.0	4.803589136907311	0.0
35	0.0	0.0	0.0	4.988869588431696	0.0
36	0.0	0.0	0.0	5.144323253848394	0.0
37	0.0	0.0	0.0	5.292925617901742	0.0
38	0.0	0.0	0.0	5.434794806477314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	25	4.4437365E-5	44.0	18
GTCGCAT	25	4.4437365E-5	44.0	16
CGAACCG	20	7.857539E-4	44.0	21
ACACGAT	20	7.857539E-4	44.0	43
GTCGATA	50	2.7284841E-11	44.0	1
CTCCGTA	25	4.4437365E-5	44.0	18
ACGTTCA	20	7.857539E-4	44.0	36
GCGAACC	20	7.857539E-4	44.0	20
CGGTTTA	30	2.5284298E-6	44.0	1
CGCATAC	20	7.857539E-4	44.0	26
GACCGAC	20	7.857539E-4	44.0	9
CGACCGT	30	2.5284298E-6	44.0	22
CGCGCCA	30	2.5284298E-6	44.0	14
CCTACGC	25	4.4437365E-5	44.0	44
CGACATA	25	4.4437365E-5	44.0	15
CGACACT	20	7.857539E-4	44.0	35
CCGTCGA	35	1.4466241E-7	44.0	18
TCGGCCG	45	4.802132E-10	44.0	23
CGTTACC	30	2.5284298E-6	44.0	33
CCCGTAC	30	2.5284298E-6	44.0	29
>>END_MODULE