FastQCFastQC Report
Thu 26 May 2016
SRR1546416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546416_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences654418
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27490.42006790766757635No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.3086712162562766No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.2553413873090288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA207.0310564E-445.0000041
CACGGTA207.0310564E-445.00000435
ACGTTAT207.0310564E-445.00000426
TAGACGC207.0310564E-445.00000412
TGGACGA207.0310564E-445.0000041
CGCATGA207.0310564E-445.00000432
ATTACGT207.0310564E-445.00000439
GGCAACG207.0310564E-445.0000041
TACTGTT207.0310564E-445.00000437
CGAGGTA207.0310564E-445.0000045
AGACCGC207.0310564E-445.00000430
GTACTCG207.0310564E-445.00000423
ACTCGAC207.0310564E-445.00000425
TCATCGC207.0310564E-445.00000444
CTCGACT207.0310564E-445.00000426
ATACGCA207.0310564E-445.00000429
GCGCTAC207.0310564E-445.00000415
GCCCGGA207.0310564E-445.00000412
GTACGAC207.0310564E-445.00000435
GACTAAC207.0310564E-445.0000049