##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546416_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	654418
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.058873686237238	31.0	31.0	33.0	30.0	34.0
2	31.217779767671427	31.0	31.0	33.0	30.0	34.0
3	31.107246438820447	31.0	31.0	34.0	28.0	34.0
4	35.060806701527156	35.0	35.0	37.0	32.0	37.0
5	32.82588804097687	35.0	35.0	37.0	28.0	37.0
6	34.01827272477224	35.0	35.0	37.0	28.0	37.0
7	15.381532904046038	0.0	0.0	35.0	0.0	37.0
8	24.99210137862956	17.0	17.0	35.0	17.0	37.0
9	32.88713330012316	32.0	32.0	37.0	28.0	39.0
10	35.61954591713553	37.0	35.0	37.0	32.0	39.0
11	36.564145546118844	37.0	35.0	39.0	33.0	39.0
12	36.284979936370945	37.0	35.0	39.0	32.0	39.0
13	35.80706062486056	37.0	35.0	39.0	31.0	39.0
14	36.81007398940738	39.0	35.0	40.0	31.0	41.0
15	37.24764141573123	39.0	35.0	41.0	32.0	41.0
16	37.37827046322076	39.0	35.0	41.0	32.0	41.0
17	37.37845077610946	39.0	35.0	41.0	32.0	41.0
18	37.2659263651061	39.0	35.0	40.0	32.0	41.0
19	37.14149060692096	39.0	35.0	40.0	32.0	41.0
20	37.21187986883001	39.0	35.0	40.0	32.0	41.0
21	37.058300657989236	39.0	35.0	40.0	32.0	41.0
22	37.07610731978644	39.0	35.0	40.0	32.0	41.0
23	37.055203249299375	38.0	35.0	40.0	32.0	41.0
24	36.890528683501984	38.0	35.0	40.0	32.0	41.0
25	36.6399594754423	38.0	35.0	40.0	31.0	41.0
26	36.491846190049785	38.0	35.0	40.0	31.0	41.0
27	36.394969270405156	38.0	35.0	40.0	31.0	41.0
28	36.38095834772271	38.0	35.0	40.0	31.0	41.0
29	36.44416412751483	38.0	35.0	40.0	31.0	41.0
30	36.181883750141346	38.0	35.0	40.0	30.0	41.0
31	36.01092115436923	38.0	35.0	40.0	30.0	41.0
32	35.82914284142551	38.0	34.0	40.0	30.0	41.0
33	35.54164463691402	38.0	34.0	40.0	29.0	41.0
34	35.333348104728174	38.0	34.0	40.0	27.0	41.0
35	35.119550501361516	38.0	34.0	40.0	26.0	41.0
36	34.86743793722055	38.0	34.0	40.0	24.0	41.0
37	34.66912126500188	38.0	34.0	40.0	24.0	41.0
38	34.6408488152832	38.0	34.0	40.0	24.0	41.0
39	34.55216696362264	37.0	33.0	40.0	23.0	41.0
40	34.3964835930552	37.0	33.0	40.0	23.0	41.0
41	34.28799482899309	37.0	33.0	40.0	23.0	41.0
42	34.23977335586735	37.0	33.0	40.0	23.0	41.0
43	34.11972011772292	37.0	33.0	40.0	23.0	41.0
44	33.820148590044894	37.0	33.0	40.0	21.0	41.0
45	33.78773658426266	37.0	33.0	40.0	21.0	41.0
46	33.80149690259131	36.0	33.0	40.0	22.0	41.0
47	33.769387455724015	36.0	33.0	40.0	22.0	41.0
48	33.799278137215055	36.0	33.0	40.0	22.0	41.0
49	33.741776662622364	36.0	33.0	40.0	21.0	41.0
50	33.584200006723535	36.0	33.0	40.0	20.0	41.0
51	33.38876834072413	36.0	33.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	8.0
14	10.0
15	18.0
16	65.0
17	181.0
18	420.0
19	819.0
20	1427.0
21	2302.0
22	3661.0
23	5538.0
24	8237.0
25	11396.0
26	13250.0
27	13401.0
28	14055.0
29	15419.0
30	18704.0
31	24632.0
32	33065.0
33	51582.0
34	65713.0
35	65787.0
36	70675.0
37	110177.0
38	104135.0
39	19738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.4658765498504	23.908419389442223	29.70272822569061	16.922975835016764
2	32.171639533142425	24.085828935023184	31.17044457823593	12.572086953598467
3	26.981226066520176	23.145298570638335	36.55614607177675	13.317329291064734
4	24.62050249229086	27.93612033898823	33.01284500120718	14.430532167513729
5	20.676387263186527	36.09329205492514	30.95697245491414	12.273348226974196
6	20.627947275288882	38.73869606276111	30.016289282996496	10.617067378953513
7	36.94794458587631	56.8112429670333	5.555623470014578	0.6851889770758139
8	86.81622449260259	3.126136506025201	8.664339917300563	1.3932990840716484
9	83.39425260307632	4.119538276758891	10.227408170313163	2.258800949851624
10	54.534105113245666	21.9893095850053	15.130237860205556	8.346347441543479
11	50.70306745841343	18.233300428777937	20.498519295007167	10.565112817801467
12	43.64993016695751	20.835765519897066	24.05129443261035	11.46300988053507
13	20.69075117126974	43.83711939463768	23.736969337640467	11.735160096452114
14	13.59268846517058	44.56937309181593	28.97154418124196	12.866394261771527
15	11.523063240925524	22.6550920054155	54.30351854625025	11.51832620740872
16	13.319774211589536	16.42787942874432	51.647418011118276	18.60492834854787
17	14.427476016857726	16.781017637045437	31.489659514255415	37.301846831841424
18	20.532289759755997	21.478474002854444	36.99669018883955	20.99254604855001
19	28.740957614246554	23.089676628699088	27.92083958570822	20.248526171346143
20	32.095846996873554	21.46074832904963	26.536556146071778	19.906848528005035
21	21.5368464803841	27.341240613797297	29.580329391917704	21.5415835139009
22	21.723730092998665	24.40810002169867	27.576717021842306	26.291452863460357
23	18.093939958864212	29.850187494842746	27.392583944818146	24.663288601474896
24	19.199044036074802	21.80838546616994	42.460629139174046	16.53194135858121
25	15.012423252416651	24.42246392978188	39.98423026261503	20.58088255518644
26	15.160799366765584	36.13623097164198	29.955777500007642	18.747192161584795
27	16.602996861333278	34.35205021866758	32.0324318707615	17.012521049237645
28	13.184539545061414	28.21728619934048	42.83592443973118	15.762249815866921
29	14.042401034201383	23.87159277403739	42.01275026053684	20.073255931224384
30	17.003658212335235	29.674458832122593	34.46421094774288	18.857672007799298
31	28.73026108695054	26.545418982974184	26.18250109257386	18.541818837501413
32	29.82451582933232	26.977711493265772	27.9005161838458	15.297256493556105
33	26.847061052721656	27.80256655532091	27.3430743041909	18.007298087766536
34	18.286630257725182	28.87298332258587	29.558172299661685	23.282214120027263
35	20.202989526571702	25.887582554269596	32.171639533142425	21.737788386016277
36	29.408573725050353	24.221674831682503	27.71149326577203	18.658258177495117
37	20.240121757042136	31.890320865257372	29.766907389466674	18.10264998823382
38	19.70254485665125	32.35485576496979	25.52267816594287	22.41992121243609
39	19.802480983102544	31.627033486242738	28.73927673138575	19.831208799268968
40	23.755764664174887	26.534111225546976	25.840670641699955	23.869453468578186
41	16.711795824686973	24.19630878123768	28.183210119526052	30.908685274549295
42	22.11124999617981	25.09053846318408	24.750694510236578	28.047517030399533
43	22.42282455555929	27.01789987439221	26.43249421623488	24.126781353813616
44	20.036887738417953	31.799247575708495	28.41593599198066	19.747928693892895
45	16.411834637800304	38.59979401544579	22.719423976724357	22.268947370029554
46	21.425144173907196	33.6992564385454	26.10793101656739	18.767668370980015
47	21.858200721862787	27.819986614060127	27.58282932315431	22.738983340922776
48	23.008230213716615	23.338294484564912	30.39922496019364	23.254250341524834
49	20.203142334104502	23.35785384876333	33.68367007019978	22.755333746932386
50	18.477181251126957	33.823794577777505	26.94027364772974	20.758750523365798
51	17.31981699769872	35.65473443578875	24.549294182005994	22.47615438450654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	383.0
2	517.0
3	3709.5
4	6902.0
5	5440.0
6	3978.0
7	4210.0
8	4442.0
9	4763.5
10	5085.0
11	5027.5
12	4970.0
13	4854.5
14	4739.0
15	4524.0
16	4309.0
17	4065.0
18	3821.0
19	3631.0
20	3441.0
21	3543.5
22	3646.0
23	3712.0
24	3778.0
25	4163.5
26	5048.5
27	5548.0
28	6555.5
29	7563.0
30	8844.0
31	10125.0
32	12130.0
33	14135.0
34	15386.0
35	16637.0
36	19507.0
37	22377.0
38	31104.0
39	39831.0
40	51648.5
41	63466.0
42	69209.5
43	74953.0
44	70836.0
45	66719.0
46	62346.5
47	57974.0
48	54503.0
49	51032.0
50	47017.0
51	43002.0
52	37867.5
53	32733.0
54	29038.5
55	25344.0
56	22694.5
57	20045.0
58	17849.0
59	15653.0
60	13743.0
61	11833.0
62	9982.0
63	8131.0
64	6590.0
65	5049.0
66	4152.5
67	3256.0
68	2570.5
69	1885.0
70	1486.5
71	1088.0
72	882.5
73	677.0
74	535.5
75	331.5
76	269.0
77	195.0
78	121.0
79	98.5
80	76.0
81	59.5
82	43.0
83	33.5
84	24.0
85	14.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	654418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.794496667186994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8713717682538	19.82858342878315
2	5.780461094058749	2.98208168851005
3	2.5649835259293408	1.984873770329218
4	1.5687356282094438	1.6185898373418397
5	1.1381723954999823	1.4679292031204265
6	0.9598024698274942	1.4854572965473887
7	0.7440527603848954	1.3434726513571629
8	0.6285651946741317	1.2970818255305308
9	0.5981700399031663	1.3886545590624002
>10	8.276398827894482	50.70859422984818
>50	0.811254921423249	13.016528467749788
>100	0.05625490331037128	1.8944824166901428
>500	0.0	0.0
>1k	0.0017764706308538297	0.9836706251297687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2749	0.42006790766757635	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2020	0.3086712162562766	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1671	0.2553413873090288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014058293017612597	0.0
2	0.0	0.0	0.0	0.06799935209606092	0.0
3	0.0	0.0	0.0	0.08541941083527653	0.0
4	0.0	0.0	0.0	0.12239883377290967	0.0
5	0.0	0.0	0.0	0.1937599515905736	0.0
6	0.0	0.0	0.0	0.2664963372034388	0.0
7	0.0	0.0	0.0	0.31142175184667903	0.0
8	0.0	0.0	0.0	0.599311143642137	0.0
9	0.0	0.0	0.0	0.7061236090694327	0.0
10	0.0	0.0	0.0	0.9101216653576155	0.0
11	0.0	0.0	0.0	1.1543081027722342	0.0
12	0.0	0.0	0.0	1.3642656528396224	0.0
13	0.0	0.0	0.0	1.4544220971917032	0.0
14	0.0	0.0	0.0	1.488803792071734	0.0
15	0.0	0.0	0.0	1.545953809338985	0.0
16	0.0	0.0	0.0	1.6845502415887093	0.0
17	0.0	0.0	0.0	1.8434700757008518	0.0
18	0.0	0.0	0.0	2.06473538319545	0.0
19	0.0	0.0	0.0	2.174145576680348	0.0
20	0.0	0.0	0.0	2.274387318197238	0.0
21	0.0	0.0	0.0	2.4091635621269587	0.0
22	0.0	0.0	0.0	2.5719035845591045	0.0
23	0.0	0.0	0.0	2.749771552738464	0.0
24	0.0	0.0	0.0	2.877518650159378	0.0
25	0.0	0.0	0.0	2.989220956636278	0.0
26	0.0	0.0	0.0	3.095574999465174	0.0
27	0.0	0.0	0.0	3.193219012924461	0.0
28	0.0	0.0	0.0	3.294530407170952	0.0
29	0.0	0.0	0.0	3.4054686759838515	0.0
30	0.0	0.0	0.0	3.540550534979172	0.0
31	0.0	0.0	0.0	3.670436937859289	0.0
32	0.0	0.0	0.0	3.792377349033798	0.0
33	0.0	0.0	0.0	3.911108802019504	0.0
34	0.0	0.0	0.0	4.03992555217002	0.0
35	0.0	0.0	0.0	4.199915039011763	0.0
36	0.0	0.0	0.0	4.334385667875884	0.0
37	0.0	0.0	0.0	4.4641192632232	0.0
38	0.0	0.0	0.0	4.593700051037716	0.0
39	0.0	0.0	0.0	4.726184181975435	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.0310564E-4	45.000004	1
CACGGTA	20	7.0310564E-4	45.000004	35
ACGTTAT	20	7.0310564E-4	45.000004	26
TAGACGC	20	7.0310564E-4	45.000004	12
TGGACGA	20	7.0310564E-4	45.000004	1
CGCATGA	20	7.0310564E-4	45.000004	32
ATTACGT	20	7.0310564E-4	45.000004	39
GGCAACG	20	7.0310564E-4	45.000004	1
TACTGTT	20	7.0310564E-4	45.000004	37
CGAGGTA	20	7.0310564E-4	45.000004	5
AGACCGC	20	7.0310564E-4	45.000004	30
GTACTCG	20	7.0310564E-4	45.000004	23
ACTCGAC	20	7.0310564E-4	45.000004	25
TCATCGC	20	7.0310564E-4	45.000004	44
CTCGACT	20	7.0310564E-4	45.000004	26
ATACGCA	20	7.0310564E-4	45.000004	29
GCGCTAC	20	7.0310564E-4	45.000004	15
GCCCGGA	20	7.0310564E-4	45.000004	12
GTACGAC	20	7.0310564E-4	45.000004	35
GACTAAC	20	7.0310564E-4	45.000004	9
>>END_MODULE