FastQCFastQC Report
Thu 26 May 2016
SRR1546415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546415_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1591938
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58920.3701149165356942No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA48840.30679586767826383No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.15050837406984444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.142530676445942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCT207.857395E-444.00000440
CGAAACC207.857395E-444.00000444
CGTGAAT207.857395E-444.00000411
ATTACGA207.857395E-444.00000435
AATATCG207.857395E-444.00000441
TCTAACG207.857395E-444.0000041
AACGCCC207.857395E-444.00000439
CGTAAGC207.857395E-444.00000439
CCGAATC207.857395E-444.00000413
CGTCAAG207.857395E-444.0000042
ATCGCAC207.857395E-444.00000411
CGCCCAT207.857395E-444.00000438
ATCGAGT207.857395E-444.00000424
GTACGAT207.857395E-444.00000439
ACGCGCT207.857395E-444.00000410
TCGCCCG254.4436158E-544.024
CGTATTA302.5283334E-644.016
TACCACG502.7284841E-1144.028
ACGTTGC254.4436158E-544.015
TCACACG302.5283334E-644.025
Produced by FastQC (version 0.11.5)