FastQCFastQC Report
Thu 26 May 2016
SRR1546414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546414_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences611826
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19760.3229676411267256No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.2698479633098299No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.21182493061752852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA207.030696E-445.0000047
GATCGCC207.030696E-445.00000427
GATCGAT207.030696E-445.00000445
CGAAACG207.030696E-445.00000445
ACCGCAA207.030696E-445.00000432
TAGCGGA207.030696E-445.0000041
TAGCGCG207.030696E-445.0000041
CACGCTA207.030696E-445.00000442
ATATGCG207.030696E-445.0000041
CGAATAT207.030696E-445.00000414
CGTACCA207.030696E-445.00000420
CGTACAG351.210683E-745.0000042
CACAACG207.030696E-445.00000412
TTTCTAC207.030696E-445.00000441
GTGGTAC207.030696E-445.00000432
AATCGGC207.030696E-445.00000410
TACGTAA207.030696E-445.0000041
ATCGAAA207.030696E-445.00000443
AATCGAA207.030696E-445.00000442
AACGTCC253.888659E-545.021