##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546414_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	611826
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.025886444838893	31.0	31.0	33.0	30.0	33.0
2	31.16392078793644	31.0	31.0	33.0	30.0	34.0
3	31.104222442328375	31.0	31.0	34.0	28.0	34.0
4	35.08228483261581	35.0	35.0	37.0	32.0	37.0
5	32.80598732319319	35.0	35.0	37.0	28.0	37.0
6	33.993454021241334	35.0	35.0	37.0	28.0	37.0
7	15.219833743580692	0.0	0.0	35.0	0.0	37.0
8	24.87913230232125	17.0	17.0	35.0	17.0	37.0
9	32.71185761965003	32.0	32.0	37.0	27.0	39.0
10	35.551305436513	37.0	35.0	37.0	32.0	39.0
11	36.4990765348318	37.0	35.0	39.0	32.0	39.0
12	36.24295306181823	37.0	35.0	39.0	32.0	39.0
13	35.79222033715468	37.0	35.0	39.0	31.0	39.0
14	36.80418942640555	39.0	35.0	40.0	31.0	41.0
15	37.25290850666693	39.0	35.0	41.0	32.0	41.0
16	37.35408596561768	39.0	35.0	41.0	32.0	41.0
17	37.31679758624184	39.0	35.0	41.0	32.0	41.0
18	37.18395099260247	39.0	35.0	40.0	32.0	41.0
19	37.11823132720741	39.0	35.0	40.0	32.0	41.0
20	37.19136976852896	39.0	35.0	40.0	32.0	41.0
21	37.03135858887985	39.0	35.0	40.0	32.0	41.0
22	37.08352374694766	39.0	35.0	40.0	32.0	41.0
23	37.071659262600804	39.0	35.0	40.0	32.0	41.0
24	36.910231013392696	38.0	35.0	40.0	32.0	41.0
25	36.6549966820632	38.0	35.0	40.0	31.0	41.0
26	36.48747356274497	38.0	35.0	40.0	31.0	41.0
27	36.36786112391432	38.0	35.0	40.0	31.0	41.0
28	36.3181476432842	38.0	35.0	40.0	31.0	41.0
29	36.390140334016536	38.0	35.0	40.0	31.0	41.0
30	36.15711493136938	38.0	35.0	40.0	30.0	41.0
31	35.99762514178868	38.0	34.0	40.0	30.0	41.0
32	35.84567507755473	38.0	34.0	40.0	30.0	41.0
33	35.626017854749556	38.0	34.0	40.0	29.0	41.0
34	35.471524583786895	38.0	34.0	40.0	27.0	41.0
35	35.25300820821606	38.0	34.0	40.0	27.0	41.0
36	35.051988637292304	38.0	34.0	40.0	25.0	41.0
37	34.874227312994215	38.0	34.0	40.0	25.0	41.0
38	34.822925145384474	38.0	34.0	40.0	25.0	41.0
39	34.72851921951666	38.0	34.0	40.0	24.0	41.0
40	34.570778293174854	37.0	33.0	40.0	23.0	41.0
41	34.4706926479097	37.0	33.0	40.0	23.0	41.0
42	34.43521360648289	37.0	33.0	40.0	23.0	41.0
43	34.36181855625619	37.0	33.0	40.0	23.0	41.0
44	34.13252951002409	37.0	33.0	40.0	23.0	41.0
45	34.05451549950476	37.0	33.0	40.0	23.0	41.0
46	34.043621879423235	36.0	33.0	40.0	23.0	41.0
47	34.00578595875298	36.0	33.0	40.0	23.0	41.0
48	33.98016429507736	36.0	33.0	40.0	23.0	41.0
49	33.907308613886954	36.0	33.0	40.0	23.0	41.0
50	33.77752498259309	36.0	33.0	40.0	23.0	41.0
51	33.56291821530958	36.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	11.0
15	25.0
16	90.0
17	203.0
18	384.0
19	800.0
20	1271.0
21	2108.0
22	3210.0
23	4896.0
24	7067.0
25	9479.0
26	11168.0
27	12148.0
28	12956.0
29	14857.0
30	18120.0
31	23493.0
32	31581.0
33	48452.0
34	61147.0
35	61276.0
36	68395.0
37	104453.0
38	96042.0
39	18192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.262077780283935	25.157642859244294	29.608908415137634	15.971370945334131
2	31.701660275960812	25.51624154579897	30.155959374070406	12.626138804169813
3	28.781385557331664	23.83733283646004	34.1873343074665	13.193947298741799
4	26.946223272629798	27.52073301886484	30.500992112136455	15.032051596368904
5	20.88845521439102	36.70389947468725	29.225629509043422	13.182015801878311
6	21.378790701931592	40.13837267458395	28.640659272407515	9.84217735107694
7	37.31256925988761	57.30207608045424	4.558322137339702	0.8270325223184369
8	87.47274551915088	3.405379960969295	7.603632405291701	1.5182421145881344
9	83.08980004118818	5.141167586862932	8.999944428644746	2.769087943304142
10	55.32618751082824	21.50595103836712	13.829095200269359	9.338766250535283
11	50.84255981275723	18.45018028001425	18.990693432446477	11.716566474782047
12	44.08148722022274	21.17415735846466	22.207948011362706	12.536407409949888
13	21.26911899788502	41.75795078993047	23.620277660642078	13.352652551542432
14	14.433025075756834	43.74266539833221	28.110116274888615	13.71419325102235
15	13.672841624906429	22.202227430674736	50.91153367133793	13.21339727308091
16	15.5576912390124	17.016766204770637	49.62522024235649	17.800322313860477
17	16.370013696704618	18.063632470669766	29.35082850352878	36.215525329096835
18	21.323546237002024	23.21411643179597	34.6730933304567	20.78924400074531
19	31.130255987813527	22.839173228989942	26.36664672635684	19.66392405683969
20	33.490240689346315	22.36289402542553	24.823397501904136	19.323467783324016
21	22.839827009639997	27.445875134433646	27.86331407949319	21.850983776433168
22	22.722473382955286	25.007273309731854	25.90540447774367	26.364848829569194
23	20.604387521942513	29.874179913897088	25.895924658317888	23.62550790584251
24	20.52593384393602	22.7649691252088	38.840781529389076	17.868315501466103
25	17.87370919182905	24.386018247017944	37.04451919336543	20.695753367787574
26	16.602105827473824	35.05751635268851	28.212596391784594	20.127781428053073
27	18.830844063508252	33.29721195241785	29.80847495856665	18.063469025507253
28	15.040060409332066	28.854445544975206	39.753295871702086	16.352198173990644
29	16.507144188053466	24.51759160284133	38.74794467708139	20.22731953202381
30	18.700578268984973	29.257828206058583	31.491306351805903	20.550287173150537
31	29.82089679091768	25.927469574683	25.566746101015646	18.684887533383673
32	31.37803885418403	25.876474683978778	27.162134332310167	15.583352129527023
33	28.63542902720708	27.345029469162803	24.726474520533614	19.2930669830965
34	20.622203044656487	27.24205901677928	29.09797883712036	23.037759101443875
35	21.05435205434225	25.549420913789216	30.84798619215267	22.548240839715866
36	30.804836669249102	25.44677735173072	25.44955591949345	18.29883005952673
37	21.572963554997663	32.43765384275922	28.19298297228297	17.796399629960153
38	21.62379500053937	32.882551575121035	23.369389336183815	22.124264088155783
39	22.316639044434204	30.80385599827402	26.16626295711526	20.71324200017652
40	24.137091264509845	25.621009894970136	25.625913249845546	24.615985590674473
41	17.187729844759787	25.134433646167377	27.104438189942893	30.573398319129947
42	22.353904541487285	26.20156711221818	24.462674028236787	26.981854318057746
43	23.175870263767802	26.419602959011222	25.616760320744785	24.787766456476188
44	20.974100479548106	30.217087864850463	27.492293560587488	21.316518095013944
45	17.602717112381626	37.09306240663195	22.170192178822084	23.134028302164342
46	21.656974368529614	33.1685806095197	25.667264875961465	19.50718014598922
47	22.8628727775544	26.845867942846496	27.464507882960188	22.826751396638915
48	23.779963584417793	23.637766293031028	30.095811554265428	22.486458568285755
49	21.17072501005188	23.999143547348428	32.121550898458054	22.708580544141636
50	19.474164223161488	32.994511511442795	25.595349004455514	21.935975260940204
51	19.18960619522544	34.023071919140406	23.458793840078716	23.328528045555437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	545.5
2	808.0
3	3313.0
4	5818.0
5	4497.0
6	3176.0
7	3282.0
8	3388.0
9	3484.0
10	3580.0
11	3593.5
12	3607.0
13	3476.5
14	3346.0
15	3294.0
16	3242.0
17	3077.0
18	2912.0
19	2794.5
20	2677.0
21	2814.0
22	2951.0
23	3017.0
24	3083.0
25	3408.5
26	4879.0
27	6024.0
28	7437.5
29	8851.0
30	9643.0
31	10435.0
32	11545.0
33	12655.0
34	13661.5
35	14668.0
36	16392.5
37	18117.0
38	25318.5
39	32520.0
40	42567.0
41	52614.0
42	57577.0
43	62540.0
44	59440.5
45	56341.0
46	53775.5
47	51210.0
48	48215.0
49	45220.0
50	41831.5
51	38443.0
52	34964.5
53	31486.0
54	29474.5
55	27463.0
56	26078.0
57	24693.0
58	23391.5
59	22090.0
60	20152.0
61	18214.0
62	15471.5
63	12729.0
64	10516.5
65	8304.0
66	6854.0
67	5404.0
68	4542.0
69	3680.0
70	3032.0
71	2384.0
72	1835.0
73	1286.0
74	1070.0
75	639.0
76	424.0
77	315.5
78	207.0
79	202.5
80	198.0
81	146.5
82	95.0
83	69.5
84	44.0
85	31.0
86	18.0
87	13.5
88	9.0
89	5.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	611826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.428588277294484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0786930643342	18.83716586053025
2	5.8116759152059245	2.955654280976801
3	2.632432881531802	2.0081715573625236
4	1.9196371284250362	1.9525464872211247
5	1.438797071782185	1.8293289176463046
6	1.1494462020933645	1.7537276531959196
7	0.9640376868165231	1.7159882195296912
8	0.8334906186053986	1.6955591818803333
9	0.7454936219170425	1.706116533756976
>10	9.905935270336592	55.77045434693948
>50	0.48584030663817135	7.585986806471248
>100	0.03196317806830075	1.3064601530762512
>500	6.392635613660149E-4	0.08257829510547912
>1k	0.0019177906840980447	0.8002617063076253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1976	0.3229676411267256	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1651	0.2698479633098299	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.21182493061752852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01062393556337913	0.0
2	0.0	0.0	0.0	0.03971717449078659	0.0
3	0.0	0.0	0.0	0.05066800037919278	0.0
4	0.0	0.0	0.0	0.07453099410616744	0.0
5	0.0	0.0	0.0	0.12617966546044138	0.0
6	0.0	0.0	0.0	0.18077034973995876	0.0
7	0.0	0.0	0.0	0.21133459512998795	0.0
8	0.0	0.0	0.0	0.45715611955032964	0.0
9	0.0	0.0	0.0	0.547868184745336	0.0
10	0.0	0.0	0.0	0.7212835021721862	0.0
11	0.0	0.0	0.0	0.9000925099619826	0.0
12	0.0	0.0	0.0	1.0682775821884	0.0
13	0.0	0.0	0.0	1.1331653117062694	0.0
14	0.0	0.0	0.0	1.1568648602707305	0.0
15	0.0	0.0	0.0	1.1993606025242471	0.0
16	0.0	0.0	0.0	1.2854962031688748	0.0
17	0.0	0.0	0.0	1.3950044620529367	0.0
18	0.0	0.0	0.0	1.566948773017165	0.0
19	0.0	0.0	0.0	1.6455658961861706	0.0
20	0.0	0.0	0.0	1.722548567730041	0.0
21	0.0	0.0	0.0	1.8276438072262375	0.0
22	0.0	0.0	0.0	1.9391134080604617	0.0
23	0.0	0.0	0.0	2.055976699257632	0.0
24	0.0	0.0	0.0	2.147179099940179	0.0
25	0.0	0.0	0.0	2.226613448921752	0.0
26	0.0	0.0	0.0	2.2995099914027843	0.0
27	0.0	0.0	0.0	2.369137630633546	0.0
28	0.0	0.0	0.0	2.444649295714795	0.0
29	0.0	0.0	0.0	2.5296407802218277	0.0
30	0.0	0.0	0.0	2.6319574519552944	0.0
31	0.0	0.0	0.0	2.74767662701487	0.0
32	0.0	0.0	0.0	2.8432920470852823	0.0
33	0.0	0.0	0.0	2.9382536865056403	0.0
34	0.0	0.0	0.0	3.046781274414621	0.0
35	0.0	0.0	0.0	3.1664231333745216	0.0
36	0.0	0.0	0.0	3.279527185833881	0.0
37	0.0	0.0	0.0	3.388545109230402	0.0
38	0.0	0.0	0.0	3.4947844648641935	0.0
39	0.0	0.0	0.0	3.6010238204979848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACA	20	7.030696E-4	45.000004	7
GATCGCC	20	7.030696E-4	45.000004	27
GATCGAT	20	7.030696E-4	45.000004	45
CGAAACG	20	7.030696E-4	45.000004	45
ACCGCAA	20	7.030696E-4	45.000004	32
TAGCGGA	20	7.030696E-4	45.000004	1
TAGCGCG	20	7.030696E-4	45.000004	1
CACGCTA	20	7.030696E-4	45.000004	42
ATATGCG	20	7.030696E-4	45.000004	1
CGAATAT	20	7.030696E-4	45.000004	14
CGTACCA	20	7.030696E-4	45.000004	20
CGTACAG	35	1.210683E-7	45.000004	2
CACAACG	20	7.030696E-4	45.000004	12
TTTCTAC	20	7.030696E-4	45.000004	41
GTGGTAC	20	7.030696E-4	45.000004	32
AATCGGC	20	7.030696E-4	45.000004	10
TACGTAA	20	7.030696E-4	45.000004	1
ATCGAAA	20	7.030696E-4	45.000004	43
AATCGAA	20	7.030696E-4	45.000004	42
AACGTCC	25	3.888659E-5	45.0	21
>>END_MODULE