FastQCFastQC Report
Thu 26 May 2016
SRR1546413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546413_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1342227
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.3243117594862866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31170.23222599455978757No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.14557895199545234No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17640.1314233732446151No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.13105085801432992No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14280.10639034976945032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA207.8569533E-444.00000440
ACGTTAG408.314601E-944.0000042
ATTACGC207.8569533E-444.00000413
TGACGTC207.8569533E-444.00000437
TCGTGCA207.8569533E-444.0000041
TCTAACG207.8569533E-444.0000041
TATTACG207.8569533E-444.0000041
CGTACGT207.8569533E-444.00000434
GATTCGC207.8569533E-444.00000410
CGATCGA207.8569533E-444.0000045
TCGACGC207.8569533E-444.00000440
ATCGCCT207.8569533E-444.00000439
TAGTCAT207.8569533E-444.00000439
ACAACGA454.802132E-1044.013
CGTATAA254.4432396E-544.041
CAACGAC302.528035E-644.012
GCGAATA254.4432396E-544.01
TTTCGTA302.528035E-644.01
CGCGATT254.4432396E-544.016
TGCGCAA551.8189894E-1244.01
Produced by FastQC (version 0.11.5)