##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546413_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1342227
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506970877504326	31.0	31.0	33.0	30.0	34.0
2	31.699138074260166	31.0	31.0	34.0	30.0	34.0
3	31.774364544894418	31.0	31.0	34.0	30.0	34.0
4	35.68110908214482	37.0	35.0	37.0	35.0	37.0
5	27.061818902465827	35.0	25.0	37.0	0.0	37.0
6	31.31036776938625	35.0	26.0	37.0	17.0	37.0
7	15.008980597171716	0.0	0.0	32.0	0.0	37.0
8	25.08080451369254	17.0	17.0	35.0	17.0	37.0
9	33.057051452548635	32.0	32.0	37.0	28.0	39.0
10	35.98335825460224	37.0	35.0	39.0	32.0	39.0
11	36.99282461163425	39.0	37.0	39.0	34.0	39.0
12	36.97055788625918	39.0	35.0	39.0	33.0	39.0
13	36.48698916055183	39.0	35.0	39.0	33.0	39.0
14	37.81569510969456	40.0	36.0	41.0	33.0	41.0
15	38.112041405812874	40.0	36.0	41.0	33.0	41.0
16	38.17089285195425	40.0	36.0	41.0	34.0	41.0
17	38.199733726113394	40.0	36.0	41.0	34.0	41.0
18	38.1690496465948	40.0	36.0	41.0	34.0	41.0
19	38.122930025994116	40.0	36.0	41.0	34.0	41.0
20	38.14377225312857	40.0	36.0	41.0	34.0	41.0
21	37.99973104400373	40.0	36.0	41.0	34.0	41.0
22	37.92893005430527	40.0	36.0	41.0	34.0	41.0
23	37.839892954023426	40.0	35.0	41.0	33.0	41.0
24	37.70696536427892	40.0	35.0	41.0	33.0	41.0
25	37.52133431975366	39.0	35.0	41.0	33.0	41.0
26	37.249604575083055	39.0	35.0	40.0	33.0	41.0
27	37.041207634774146	39.0	35.0	40.0	32.0	41.0
28	37.07583441549008	39.0	35.0	40.0	32.0	41.0
29	37.087822700631115	39.0	35.0	40.0	33.0	41.0
30	36.908554961269594	39.0	35.0	40.0	32.0	41.0
31	36.74363203839589	38.0	35.0	40.0	32.0	41.0
32	36.53757523876364	38.0	35.0	40.0	31.0	41.0
33	36.3584475651287	38.0	35.0	40.0	31.0	41.0
34	36.19273416493633	38.0	35.0	40.0	30.0	41.0
35	36.04988127939611	38.0	35.0	40.0	30.0	41.0
36	35.84326794200981	38.0	35.0	40.0	30.0	41.0
37	35.686743747518115	38.0	35.0	40.0	29.0	41.0
38	35.545129847633824	38.0	34.0	40.0	29.0	41.0
39	35.47370973762262	38.0	34.0	40.0	29.0	41.0
40	35.2155432724867	38.0	34.0	40.0	28.0	41.0
41	35.06238512561586	38.0	34.0	40.0	27.0	40.0
42	35.06780298712513	38.0	34.0	40.0	27.0	41.0
43	34.930281539560745	37.0	34.0	40.0	27.0	41.0
44	34.731593091183534	37.0	34.0	40.0	26.0	40.0
45	34.515964885224335	37.0	34.0	40.0	26.0	40.0
46	34.46421357937219	37.0	34.0	40.0	26.0	40.0
47	34.297144968772045	36.0	33.0	39.0	26.0	40.0
48	34.234483436855314	36.0	33.0	39.0	26.0	40.0
49	34.09447880276585	36.0	33.0	39.0	24.0	40.0
50	34.202823367433375	36.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	6.0
14	15.0
15	23.0
16	69.0
17	198.0
18	462.0
19	937.0
20	1602.0
21	2732.0
22	4416.0
23	6606.0
24	9882.0
25	14571.0
26	18898.0
27	21004.0
28	22697.0
29	26001.0
30	32547.0
31	43503.0
32	60689.0
33	110112.0
34	113453.0
35	141192.0
36	184043.0
37	338678.0
38	150361.0
39	37524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.83721754963952	23.49840973248191	28.992264348727897	14.67210836915067
2	32.03809787763173	25.87460988342508	29.737071300160107	12.350220938783082
3	30.024057033571815	24.098978786747697	32.68798794838727	13.188976231293218
4	28.0252893139536	26.216280852642658	30.846198146811233	14.912231686592506
5	18.128379178782726	48.33452165691794	22.356129030335406	11.180970133963926
6	22.67269247303176	40.198565518351224	27.040135535941385	10.088606472675634
7	38.70001124995996	56.12508167396424	3.911111905810269	1.2637951702655363
8	85.97264099142693	3.99157519555187	7.960948483378743	2.0748353296424527
9	80.12109725106112	6.557534604802317	9.72979980286494	3.591568341271633
10	49.449683250299685	24.58756976278975	14.485701747915963	11.477045238994595
11	44.87474920412121	20.567161888413807	20.874412450353034	13.683676457111948
12	39.97483287104193	21.26033822892849	24.966045236759506	13.798783663270072
13	21.680535408690186	37.532623021292224	24.997783534379803	15.789058035637787
14	15.72662448304199	40.14216671248604	27.69375075899978	16.43745804547219
15	16.299105888944272	23.077318516167534	45.32713169977954	15.296443895108652
16	17.20066724928049	18.24922311948724	44.61763919217837	19.9324704390539
17	17.39251259287736	19.749043939661473	29.181576588758833	33.67686687870234
18	21.547845483662602	24.181975180055236	32.629726566370664	21.640452769911498
19	29.257718701829123	23.04595273377752	26.214418276491237	21.48191028790212
20	31.402586894765193	21.88757937368269	26.043582791882447	20.66625093966967
21	23.1002654543531	26.884796684912466	27.576482964506006	22.438454896228432
22	23.55227543478115	25.84577720460101	25.673153646886853	24.928793713730986
23	21.55142162987334	28.293723788897108	26.19601602411515	23.958838557114408
24	21.267490521349966	23.792398752222983	36.53487822849637	18.40523249793068
25	19.11777963042019	24.719365651264653	34.37063924358547	21.792215474729684
26	18.232832449354692	32.21593664856988	28.40041215085079	21.15081875122464
27	21.034593999375666	31.15963246157319	28.45994008465036	19.345833454400783
28	16.111879734202933	28.394675416304395	37.11019075014882	18.38325409934385
29	17.603728728449063	24.428058741181633	35.88737225521466	22.08084027515465
30	19.5556340320974	28.703341536118703	30.746736580325084	20.994287851458807
31	27.606731201205157	27.12790012419658	24.679283012485964	20.586085662112296
32	28.75847379020091	26.498200378922494	26.250924769059182	18.49240106181741
33	26.198549127681087	28.606189564060326	25.50917244251531	19.686088865743276
34	20.309232342964343	28.53690173122728	28.363980161328893	22.789885764479482
35	21.94703280443621	25.382889779448632	29.330210165642622	23.339867250472533
36	28.81181797117775	26.00849185718958	25.675761253498848	19.503928918133816
37	21.69551052094765	31.953909435587274	28.03236710332902	18.318212940136057
38	20.807434211947758	31.0644175687123	25.33602736347876	22.792120855861192
39	23.110025353386572	29.85627617385137	26.53448336235227	20.49921511040979
40	23.88031234657029	27.14719641312535	26.33012150701781	22.642369733286547
41	18.53494230111598	25.778128438781216	26.943877600435695	28.743051659667106
42	22.405897065101506	26.68162687831492	24.734117254383943	26.178358802199632
43	23.56278036427519	27.613138463166067	25.51133303085097	23.31274814170777
44	21.461645459374605	30.387855407468333	26.445824737544392	21.704674395612663
45	18.946050109258717	34.713576764586016	23.111962432584058	23.228410693571206
46	22.19192431682569	32.29520788957457	25.53293891420751	19.979928879392233
47	23.597647789829885	27.231682867354035	27.358189039558884	21.812480303257196
48	23.80029607510503	24.099500308070095	29.36187396021686	22.738329656608013
49	20.616035886627223	25.369181218974134	31.247844068104726	22.766938826293913
50	19.61657752377206	31.645988346233533	27.31952195865528	21.417912171339125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	529.0
1	1248.5
2	1968.0
3	7912.5
4	13857.0
5	10307.0
6	6757.0
7	6896.5
8	7036.0
9	7125.5
10	7215.0
11	7124.0
12	7033.0
13	6707.0
14	6381.0
15	6176.5
16	5972.0
17	5562.0
18	5152.0
19	5070.0
20	4988.0
21	5516.0
22	6044.0
23	7068.5
24	8093.0
25	10064.0
26	12035.0
27	14465.0
28	16895.0
29	20182.0
30	23469.0
31	26433.5
32	29398.0
33	32910.5
34	36423.0
35	37131.5
36	37840.0
37	43857.0
38	49874.0
39	64331.5
40	78789.0
41	97170.0
42	115551.0
43	120400.5
44	125250.0
45	121637.5
46	118025.0
47	109517.0
48	101009.0
49	95777.0
50	90545.0
51	83671.5
52	76798.0
53	72792.5
54	68787.0
55	66078.5
56	63370.0
57	60517.5
58	57665.0
59	53357.5
60	49050.0
61	43275.5
62	37501.0
63	31864.5
64	26228.0
65	21698.0
66	17168.0
67	14442.5
68	11717.0
69	9528.0
70	7339.0
71	6103.0
72	4867.0
73	3897.5
74	2928.0
75	2156.5
76	1385.0
77	1045.0
78	705.0
79	511.5
80	318.0
81	204.0
82	90.0
83	87.0
84	84.0
85	74.0
86	64.0
87	42.0
88	20.0
89	14.5
90	9.0
91	7.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1342227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.771342118006228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45997549538777	13.97713674122284
2	5.5953866528991	2.100658342881895
3	2.1999371701358372	1.2388731977621483
4	1.3152268984399402	0.9875429629368139
5	1.0420256124593361	0.9780109633599585
6	0.8647830220132031	0.9739882778431889
7	0.7406065268718987	0.9731524943518548
8	0.6644491218261733	0.9978081428646319
9	0.6187052890920604	1.0452535786590311
>10	10.390870090823235	46.1595040296971
>50	1.9221314353688803	24.14376675932408
>100	0.18035971472643272	4.7313474790163665
>500	0.0012791469129534232	0.1898493795078462
>1k	0.004263823043178078	1.5031076505722392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4353	0.3243117594862866	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3117	0.23222599455978757	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1954	0.14557895199545234	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1764	0.1314233732446151	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.13105085801432992	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1428	0.10639034976945032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01028142035587125	0.0
2	0.0	0.0	0.0	0.039710123548401276	0.0
3	0.0	0.0	0.0	0.05043856218061475	0.0
4	0.0	0.0	0.0	0.07264046990561208	0.0
5	0.0	0.0	0.0	0.12270651685594165	0.0
6	0.0	0.0	0.0	0.19750757509720784	0.0
7	0.0	0.0	0.0	0.2521183078570167	0.0
8	0.0	0.0	0.0	0.4958922745556452	0.0
9	0.0	0.0	0.0	0.5876055242518591	0.0
10	0.0	0.0	0.0	0.761793645933214	0.0
11	0.0	0.0	0.0	0.9266688868574392	0.0
12	0.0	0.0	0.0	1.079176622136196	0.0
13	0.0	0.0	0.0	1.1430257326070776	0.0
14	0.0	0.0	0.0	1.1667177012532157	0.0
15	0.0	0.0	0.0	1.2111960197492675	0.0
16	0.0	0.0	0.0	1.314159229400094	0.0
17	0.0	0.0	0.0	1.4248707558408527	0.0
18	0.0	0.0	0.0	1.5717162596192746	0.0
19	0.0	0.0	0.0	1.6407060802680917	0.0
20	0.0	0.0	0.0	1.7107389435617075	0.0
21	0.0	0.0	0.0	1.815490226317903	0.0
22	0.0	0.0	0.0	1.9185279390147867	0.0
23	0.0	0.0	0.0	2.0357957335085644	0.0
24	0.0	0.0	0.0	2.126987461882379	0.0
25	0.0	0.0	0.0	2.204545132827756	0.0
26	0.0	0.0	0.0	2.284114386016672	0.0
27	0.0	0.0	0.0	2.3629386087450186	0.0
28	0.0	0.0	0.0	2.4389317157231973	0.0
29	0.0	0.0	0.0	2.521034072478053	0.0
30	0.0	0.0	0.0	2.6266048887408764	0.0
31	0.0	0.0	0.0	2.7332187476484977	0.0
32	0.0	0.0	0.0	2.8232929303314567	0.0
33	0.0	0.0	0.0	2.9211154298043476	0.0
34	0.0	0.0	0.0	3.019459450599638	0.0
35	0.0	0.0	0.0	3.1403778943502103	0.0
36	0.0	0.0	0.0	3.262115871607411	0.0
37	0.0	0.0	0.0	3.374093949831139	0.0
38	0.0	0.0	0.0	3.480260790462418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	20	7.8569533E-4	44.000004	40
ACGTTAG	40	8.314601E-9	44.000004	2
ATTACGC	20	7.8569533E-4	44.000004	13
TGACGTC	20	7.8569533E-4	44.000004	37
TCGTGCA	20	7.8569533E-4	44.000004	1
TCTAACG	20	7.8569533E-4	44.000004	1
TATTACG	20	7.8569533E-4	44.000004	1
CGTACGT	20	7.8569533E-4	44.000004	34
GATTCGC	20	7.8569533E-4	44.000004	10
CGATCGA	20	7.8569533E-4	44.000004	5
TCGACGC	20	7.8569533E-4	44.000004	40
ATCGCCT	20	7.8569533E-4	44.000004	39
TAGTCAT	20	7.8569533E-4	44.000004	39
ACAACGA	45	4.802132E-10	44.0	13
CGTATAA	25	4.4432396E-5	44.0	41
CAACGAC	30	2.528035E-6	44.0	12
GCGAATA	25	4.4432396E-5	44.0	1
TTTCGTA	30	2.528035E-6	44.0	1
CGCGATT	25	4.4432396E-5	44.0	16
TGCGCAA	55	1.8189894E-12	44.0	1
>>END_MODULE