FastQCFastQC Report
Thu 26 May 2016
SRR1546412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546412_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences513960
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12070.2348431784574675No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.22180714452486577No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.19923729473110746No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7290.14183983189353255No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6200.12063195579422523No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6060.11790800840532337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA351.2101737E-745.0000041
ATCGTAG351.2101737E-745.0000042
TCTACGG351.2101737E-745.0000042
CTGTCCG253.8877857E-545.021
ATAGGCG502.1827873E-1145.05
ACGTTAG207.0296426E-445.02
CGTTGAC207.0296426E-445.034
TACCTTA207.0296426E-445.011
TATTACG207.0296426E-445.01
AGCGAAA453.8380676E-1045.01
GCGATAT551.8189894E-1245.09
TCCGTAG207.0296426E-445.02
ACCCGTT207.0296426E-445.012
GATCTCG207.0296426E-445.013
TATACTC253.8877857E-545.043
TATCGCG406.8030204E-945.032
CGATAGT453.8380676E-1045.010
TACAACG253.8877857E-545.08
CGTACTT302.1631513E-644.99999629
ACGTACT302.1631513E-644.99999628