FastQCFastQC Report
Thu 26 May 2016
SRR1546411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546411_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1035048
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.3496456202997349No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25830.24955364388897905No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.14327837936018425No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.14289192385280683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.8561174E-444.0000041
TCGTTAA207.8561174E-444.0000041
GATCGCT207.8561174E-444.0000048
CAACGAT408.310963E-944.00000412
CGACATC207.8561174E-444.00000432
CGTTAAG800.044.0000042
GTTCGCT207.8561174E-444.00000421
GTTCGAA408.310963E-944.0000041
CGTAGGA408.310963E-944.0000044
AACGACA207.8561174E-444.00000430
CGTAATT207.8561174E-444.00000420
TATAGCG207.8561174E-444.0000041
ACCGTAT408.310963E-944.00000426
ACGTAAT207.8561174E-444.00000419
CGCACGG207.8561174E-444.0000042
GCGTGAT207.8561174E-444.00000416
CACGTCG207.8561174E-444.00000432
CGCGTAG207.8561174E-444.00000424
TAAGCGT207.8561174E-444.00000432
CGTCACT207.8561174E-444.00000442
Produced by FastQC (version 0.11.5)