##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546411_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1035048
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526800689436627	31.0	31.0	33.0	30.0	34.0
2	31.709789304457377	31.0	31.0	34.0	30.0	34.0
3	31.760647815367015	31.0	31.0	34.0	30.0	34.0
4	35.65558891954769	37.0	35.0	37.0	33.0	37.0
5	27.045816232677133	35.0	25.0	37.0	0.0	37.0
6	31.30075803247772	35.0	26.0	37.0	17.0	37.0
7	15.047076077631182	0.0	0.0	32.0	0.0	37.0
8	25.130594909608057	17.0	17.0	35.0	17.0	37.0
9	33.25391189587343	32.0	32.0	37.0	28.0	39.0
10	36.102638718204375	37.0	35.0	39.0	32.0	39.0
11	37.02707314056933	39.0	37.0	39.0	34.0	39.0
12	36.843520300507805	39.0	35.0	39.0	33.0	39.0
13	36.204012760760854	38.0	35.0	39.0	32.0	39.0
14	37.47863673955217	40.0	35.0	41.0	33.0	41.0
15	37.82830168262728	40.0	35.0	41.0	33.0	41.0
16	37.93053752096521	40.0	35.0	41.0	33.0	41.0
17	37.98927102897643	40.0	35.0	41.0	34.0	41.0
18	37.95930237051808	40.0	36.0	41.0	34.0	41.0
19	37.88344791739127	40.0	36.0	41.0	34.0	41.0
20	37.90573480650173	40.0	36.0	41.0	34.0	41.0
21	37.74909472797397	40.0	35.0	41.0	33.0	41.0
22	37.65828154829534	39.0	35.0	41.0	33.0	41.0
23	37.56365791731398	39.0	35.0	41.0	33.0	41.0
24	37.43583099527751	39.0	35.0	41.0	33.0	41.0
25	37.26123812615454	39.0	35.0	41.0	33.0	41.0
26	37.01097920096459	38.0	35.0	40.0	33.0	41.0
27	36.77823250709146	38.0	35.0	40.0	32.0	41.0
28	36.847441857768914	38.0	35.0	40.0	32.0	41.0
29	36.85575644801014	38.0	35.0	40.0	32.0	41.0
30	36.67670098391572	38.0	35.0	40.0	32.0	41.0
31	36.45497696725176	38.0	35.0	40.0	31.0	41.0
32	36.203832092811155	38.0	35.0	40.0	31.0	41.0
33	35.988406334778674	38.0	35.0	40.0	30.0	41.0
34	35.786602167242485	38.0	35.0	40.0	30.0	41.0
35	35.620514217698116	38.0	35.0	40.0	29.0	41.0
36	35.417056986729115	38.0	35.0	40.0	29.0	41.0
37	35.21102499594222	38.0	34.0	40.0	27.0	41.0
38	35.07401106035662	38.0	34.0	40.0	27.0	41.0
39	35.0192116694105	38.0	34.0	40.0	27.0	41.0
40	34.77603260911571	37.0	34.0	40.0	26.0	40.0
41	34.63866216832456	37.0	34.0	40.0	25.0	40.0
42	34.64720380117637	37.0	34.0	40.0	25.0	40.0
43	34.49889860180397	37.0	33.0	40.0	24.0	41.0
44	34.30514720090276	37.0	33.0	40.0	24.0	40.0
45	34.09213872206893	36.0	33.0	39.0	24.0	40.0
46	34.06862000602871	36.0	33.0	39.0	24.0	40.0
47	33.9291018387553	36.0	33.0	39.0	23.0	40.0
48	33.91297408429367	36.0	33.0	39.0	23.0	40.0
49	33.80557520037718	36.0	33.0	39.0	24.0	40.0
50	33.84015717145485	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	9.0
15	20.0
16	58.0
17	169.0
18	379.0
19	768.0
20	1389.0
21	2434.0
22	3873.0
23	6135.0
24	9224.0
25	13643.0
26	16743.0
27	18415.0
28	19096.0
29	21866.0
30	26526.0
31	35487.0
32	50076.0
33	97697.0
34	91460.0
35	107366.0
36	129980.0
37	244644.0
38	112262.0
39	25325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.497812661828245	23.63465269243552	30.295020134331935	15.5725145114043
2	31.499505336950556	24.662720955936344	30.78581862870128	13.051955078411822
3	28.256950402300184	24.147092695218	34.34980793161284	13.246148970868985
4	25.311289911192524	27.39728012613908	32.637520192300265	14.653909770368138
5	17.11002774750543	48.85145423207426	23.848459201892087	10.190058818528223
6	22.37171609432606	38.88553960782495	28.037443674109802	10.70530062373919
7	38.96466637296048	55.8804036141319	4.141450444810289	1.013479568097325
8	87.6352594275821	3.2296086751532296	7.646891738354163	1.4882401589105045
9	84.05194734930167	4.6348575138544295	8.98267519960427	2.3305199372396257
10	56.57302849722912	20.920189208616414	13.157747273556394	9.349035020598079
11	51.709292709130395	17.43030274924448	19.385574388820615	11.474830152804508
12	44.953470756911756	20.482238504880932	22.29877261730857	12.265518120898742
13	20.622328626305254	42.28914987517487	23.814547731119717	13.273973767400161
14	14.681734566899312	44.21331184640712	27.52481044357363	13.580143143119933
15	13.843995640781879	21.20056267921874	52.035364543480114	12.920077136519273
16	14.488700040964284	16.549184192423926	51.096470888306634	17.86564487830516
17	15.758689452083383	17.255914701540412	29.6071293312001	37.37826651517611
18	21.483834566126404	22.474030189904237	34.32942240359868	21.71271284037069
19	29.526456744035055	22.078299750349743	27.358924417031865	21.03631908858333
20	32.530955086140935	21.999076371337367	24.848026371723826	20.621942170797876
21	23.02801415972979	26.556836011469997	28.803591717485567	21.611558111314643
22	22.088540821295243	24.7490937618352	26.926673932030205	26.23569148483935
23	19.877339021958402	29.79127538046545	25.702769340165865	24.628616257410286
24	20.399633640179008	22.796527310810706	39.491308615639085	17.312530433371208
25	16.636909592598602	24.148445289493818	37.52289748881211	21.691747629095463
26	16.367936559463907	34.340629613312615	29.07169522572866	20.21973860149481
27	18.076552971456398	32.72746771164236	30.77857258793795	18.417406728963297
28	14.467155146427993	28.102078357718675	40.44643340212241	16.984333093730918
29	14.914284168463684	23.797736916548796	40.17021432822439	21.117764586763126
30	18.20475958602886	28.659926882618002	33.307054358831664	19.828259172521467
31	29.014982880021023	26.032126046328287	24.86503041404843	20.08786065960226
32	30.62659895966177	26.58408112474011	26.416939117799366	16.37238079779875
33	28.149515771249256	27.226176950247716	25.943627735138854	18.680679543364175
34	20.09472024485821	27.097777107921566	29.171787202139416	23.635715445080805
35	20.27335930314343	25.322883576413847	31.257874030962817	23.145883089479906
36	30.587277111786122	24.096080568244176	26.716828591524255	18.599813728445444
37	21.24954591477883	31.374003910929734	29.201834117838015	18.17461605645342
38	21.21234957219375	32.52293613436285	23.8961864570532	22.368527836390196
39	21.38606132275991	31.41690047224863	26.730740989789847	20.466297215201614
40	23.85609169816279	26.26003818180413	24.91333735247061	24.97053276756247
41	17.5446935794282	24.380415207797128	26.497321863333873	31.577569349440797
42	22.778557129717655	25.750496595326982	23.251578670747637	28.21936760420773
43	22.917101429112467	27.334481106190246	25.08927122220419	24.6591462424931
44	20.245727734365943	31.630513753951504	26.854986435411693	21.26877207627086
45	17.68632952288203	36.28884843987912	22.910628299363893	23.11419373787496
46	21.541513050602486	33.08957652205501	25.40114081665778	19.967769610684723
47	22.060039727626158	27.00251582535303	27.54819100176997	23.389253445250848
48	24.391235962003694	22.279932911323918	30.173190035631198	23.155641091041186
49	20.560785586755397	22.86203151931118	32.363040168185435	24.214142725747983
50	18.887723081441635	32.630853834798	26.586303243907526	21.89511983985284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	405.0
1	855.0
2	1305.0
3	6248.5
4	11192.0
5	8466.5
6	5741.0
7	5956.5
8	6172.0
9	6497.5
10	6823.0
11	6763.0
12	6703.0
13	6541.5
14	6380.0
15	5906.0
16	5432.0
17	5169.0
18	4906.0
19	4427.0
20	3948.0
21	4202.0
22	4456.0
23	4887.0
24	5318.0
25	6283.0
26	7248.0
27	8132.0
28	9016.0
29	10832.5
30	12649.0
31	15314.0
32	17979.0
33	19421.0
34	20863.0
35	21543.0
36	22223.0
37	29150.0
38	36077.0
39	52065.5
40	68054.0
41	86622.0
42	105190.0
43	108402.5
44	111615.0
45	105256.0
46	98897.0
47	91558.5
48	84220.0
49	79970.0
50	75720.0
51	70850.5
52	65981.0
53	59764.5
54	53548.0
55	48064.0
56	42580.0
57	39699.5
58	36819.0
59	34547.0
60	32275.0
61	28317.0
62	24359.0
63	19909.5
64	15460.0
65	12321.5
66	9183.0
67	7784.5
68	6386.0
69	5190.0
70	3994.0
71	3327.0
72	2660.0
73	2138.5
74	1617.0
75	1211.0
76	805.0
77	650.0
78	495.0
79	360.0
80	225.0
81	154.5
82	84.0
83	52.5
84	21.0
85	17.5
86	14.0
87	10.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1035048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.35986505573709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78127393482352	15.225366460091161
2	5.90055018043243	2.4026881085641882
3	2.291826095224318	1.3998381008995193
4	1.4743490514887958	1.2007019093346343
5	1.1130638109163804	1.1330914494340985
6	0.9666961645297744	1.1809082075834894
7	0.7642629093636847	1.0892202791224752
8	0.6843167781178694	1.1146077806285282
9	0.6081428426609149	1.1143535590069722
>10	9.4890310743961	45.023181762827804
>50	1.774201526981586	24.07705346072903
>100	0.14982146227718784	4.065286767276138
>500	4.928337574907495E-4	0.054183755506087
>1k	0.001971335029962998	0.9195183989958913
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3619	0.3496456202997349	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2583	0.24955364388897905	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1483	0.14327837936018425	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1479	0.14289192385280683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010144457068657686	0.0
2	0.0	0.0	0.0	0.042703333565206635	0.0
3	0.0	0.0	0.0	0.05903107875190329	0.0
4	0.0	0.0	0.0	0.08588973651463506	0.0
5	9.661387684435891E-5	0.0	0.0	0.1366120218579235	0.0
6	9.661387684435891E-5	0.0	0.0	0.21844397554509548	0.0
7	9.661387684435891E-5	0.0	0.0	0.27390034085375753	0.0
8	9.661387684435891E-5	0.0	0.0	0.5592977330519937	0.0
9	9.661387684435891E-5	0.0	0.0	0.6513707576846678	0.0
10	9.661387684435891E-5	0.0	0.0	0.8355168069500158	0.0
11	9.661387684435891E-5	0.0	0.0	1.0355075320178388	0.0
12	9.661387684435891E-5	0.0	0.0	1.202456311204891	0.0
13	9.661387684435891E-5	0.0	0.0	1.2717284609022963	0.0
14	9.661387684435891E-5	0.0	0.0	1.2960751578670748	0.0
15	9.661387684435891E-5	0.0	0.0	1.3423532048755227	0.0
16	9.661387684435891E-5	0.0	0.0	1.437711101320905	0.0
17	9.661387684435891E-5	0.0	0.0	1.5681398350607894	0.0
18	9.661387684435891E-5	0.0	0.0	1.747841645991297	0.0
19	9.661387684435891E-5	0.0	0.0	1.831509263338512	0.0
20	9.661387684435891E-5	0.0	0.0	1.9112157117351078	0.0
21	9.661387684435891E-5	0.0	0.0	2.0327559688053114	0.0
22	9.661387684435891E-5	0.0	0.0	2.1537165426144487	0.0
23	9.661387684435891E-5	0.0	0.0	2.2943863472998354	0.0
24	9.661387684435891E-5	0.0	0.0	2.3996954730601865	0.0
25	9.661387684435891E-5	0.0	0.0	2.4844258430526893	0.0
26	9.661387684435891E-5	0.0	0.0	2.568769757537815	0.0
27	9.661387684435891E-5	0.0	0.0	2.6510847806092084	0.0
28	9.661387684435891E-5	0.0	0.0	2.738713566907042	0.0
29	9.661387684435891E-5	0.0	0.0	2.834844374367179	0.0
30	9.661387684435891E-5	0.0	0.0	2.966142632998663	0.0
31	9.661387684435891E-5	0.0	0.0	3.087296434561489	0.0
32	9.661387684435891E-5	0.0	0.0	3.195310748873482	0.0
33	9.661387684435891E-5	0.0	0.0	3.2954993391610823	0.0
34	9.661387684435891E-5	0.0	0.0	3.404093336734142	0.0
35	9.661387684435891E-5	0.0	0.0	3.554134687473431	0.0
36	9.661387684435891E-5	0.0	0.0	3.676544469435234	0.0
37	9.661387684435891E-5	0.0	0.0	3.7976016571212154	0.0
38	9.661387684435891E-5	0.0	0.0	3.916436725639777	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.8561174E-4	44.000004	1
TCGTTAA	20	7.8561174E-4	44.000004	1
GATCGCT	20	7.8561174E-4	44.000004	8
CAACGAT	40	8.310963E-9	44.000004	12
CGACATC	20	7.8561174E-4	44.000004	32
CGTTAAG	80	0.0	44.000004	2
GTTCGCT	20	7.8561174E-4	44.000004	21
GTTCGAA	40	8.310963E-9	44.000004	1
CGTAGGA	40	8.310963E-9	44.000004	4
AACGACA	20	7.8561174E-4	44.000004	30
CGTAATT	20	7.8561174E-4	44.000004	20
TATAGCG	20	7.8561174E-4	44.000004	1
ACCGTAT	40	8.310963E-9	44.000004	26
ACGTAAT	20	7.8561174E-4	44.000004	19
CGCACGG	20	7.8561174E-4	44.000004	2
GCGTGAT	20	7.8561174E-4	44.000004	16
CACGTCG	20	7.8561174E-4	44.000004	32
CGCGTAG	20	7.8561174E-4	44.000004	24
TAAGCGT	20	7.8561174E-4	44.000004	32
CGTCACT	20	7.8561174E-4	44.000004	42
>>END_MODULE