FastQCFastQC Report
Thu 26 May 2016
SRR1546410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546410_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences398354
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10440.26207845283340947No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.2570577928174438No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.2118718526737525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCG302.161918E-645.00000441
GCGATGA600.045.0000049
TTGCATA302.161918E-645.0000041
TCCCCGC302.161918E-645.00000432
ACGGTCT600.045.00000430
AGCGTAA502.1827873E-1145.01
GGTACTA351.2092642E-745.038
CGAAATG207.027731E-445.045
TTTACGG351.2092642E-745.02
TATGGTA207.027731E-445.01
CCGGATA351.2092642E-745.020
GCCGGAT351.2092642E-745.019
TACGGGT207.027731E-445.04
CGACAAG207.027731E-445.02
ATACTCG453.8380676E-1045.044
TGATTCG207.027731E-445.015
TTAAGCC207.027731E-445.014
CGCTAAG207.027731E-445.02
CAAGGCG207.027731E-445.05
GCGATAT207.027731E-445.09