##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546410_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	398354
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991088328471662	31.0	31.0	33.0	30.0	33.0
2	31.139586900093885	31.0	31.0	33.0	30.0	34.0
3	31.081708731429835	31.0	31.0	34.0	28.0	34.0
4	35.042035475983674	35.0	35.0	37.0	32.0	37.0
5	32.70487054228149	35.0	35.0	37.0	28.0	37.0
6	33.909916807663535	35.0	35.0	37.0	28.0	37.0
7	15.480276337127279	0.0	0.0	35.0	0.0	37.0
8	25.0070439860024	17.0	17.0	35.0	17.0	37.0
9	32.75813221406086	32.0	32.0	37.0	27.0	39.0
10	35.54576331604553	37.0	35.0	37.0	32.0	39.0
11	36.440106036339536	37.0	35.0	39.0	32.0	39.0
12	36.14451216756955	37.0	35.0	39.0	32.0	39.0
13	35.614428372753885	37.0	35.0	39.0	30.0	39.0
14	36.611496307304556	38.0	35.0	40.0	31.0	41.0
15	37.0627984154797	39.0	35.0	40.0	32.0	41.0
16	37.185520918579954	39.0	35.0	41.0	32.0	41.0
17	37.193835131566395	39.0	35.0	41.0	32.0	41.0
18	37.08500228440031	38.0	35.0	40.0	32.0	41.0
19	36.99981674590942	39.0	35.0	40.0	32.0	41.0
20	37.0983647710328	39.0	35.0	40.0	32.0	41.0
21	36.926208849415346	39.0	35.0	40.0	32.0	41.0
22	36.96170491572822	39.0	35.0	40.0	32.0	41.0
23	36.972564603342754	38.0	35.0	40.0	32.0	41.0
24	36.8209758154807	38.0	35.0	40.0	32.0	41.0
25	36.55612093765846	38.0	35.0	40.0	31.0	41.0
26	36.41818583470983	38.0	35.0	40.0	31.0	41.0
27	36.30203286524046	38.0	35.0	40.0	31.0	41.0
28	36.25813723472088	38.0	35.0	40.0	31.0	41.0
29	36.33741345637298	38.0	35.0	40.0	31.0	41.0
30	36.10659112246896	38.0	35.0	40.0	30.0	41.0
31	35.95463833675575	38.0	34.0	40.0	30.0	41.0
32	35.80806267791964	38.0	34.0	40.0	30.0	41.0
33	35.587977025459764	38.0	34.0	40.0	29.0	41.0
34	35.461122519166366	38.0	34.0	40.0	28.0	41.0
35	35.25534072709198	38.0	34.0	40.0	27.0	41.0
36	35.04432238662095	38.0	34.0	40.0	25.0	41.0
37	34.85266371117147	38.0	34.0	40.0	25.0	41.0
38	34.79531773246911	38.0	33.0	40.0	25.0	41.0
39	34.69704835397661	37.0	33.0	40.0	24.0	41.0
40	34.509519171390274	37.0	33.0	40.0	23.0	41.0
41	34.42048027633713	37.0	33.0	40.0	23.0	41.0
42	34.3827374646671	37.0	33.0	40.0	23.0	41.0
43	34.27951018440884	37.0	33.0	40.0	23.0	41.0
44	34.08101337001762	37.0	33.0	40.0	23.0	41.0
45	33.988695983974054	36.0	33.0	40.0	23.0	41.0
46	33.985929600305255	36.0	33.0	40.0	23.0	41.0
47	33.95883309819909	36.0	33.0	40.0	23.0	41.0
48	33.95201504189741	36.0	33.0	40.0	23.0	41.0
49	33.874152136039804	36.0	33.0	40.0	23.0	41.0
50	33.71179654277351	36.0	33.0	40.0	23.0	41.0
51	33.496259608288106	35.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	5.0
15	10.0
16	70.0
17	110.0
18	276.0
19	515.0
20	958.0
21	1395.0
22	2170.0
23	3185.0
24	4554.0
25	6170.0
26	7245.0
27	8036.0
28	8573.0
29	10067.0
30	12076.0
31	15934.0
32	20953.0
33	32397.0
34	40334.0
35	39922.0
36	43896.0
37	66925.0
38	61233.0
39	11342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.028065489489247	24.24953684411353	30.59891453330455	15.123483133092677
2	31.1326106930017	24.39287668756935	31.424562072930108	13.049950546498842
3	28.75583024144354	23.86997494690652	33.97505736104068	13.399137450609258
4	25.615909467458593	28.022060780110152	31.36556931774251	14.996460434688744
5	21.017486958835608	37.52140056331805	28.98878886618435	12.47232361166199
6	22.37657962515752	39.648152146081124	27.432886327236577	10.542381901524776
7	38.26019068466741	56.582838379933435	4.423452507066579	0.7335184283325886
8	88.17132500238482	3.4441727709524694	6.991771138233832	1.3927310884288848
9	84.46683100960453	4.817323285319088	8.306430963414451	2.409414741661939
10	56.806257750643894	21.566998197583054	12.56144032694538	9.065303724827665
11	52.23720610311432	17.639335867093088	18.800363495785156	11.323094534007442
12	45.31170767709123	20.87515124738298	21.667411397902367	12.145729677623422
13	20.86460786134945	42.982874528685535	23.045582572284953	13.106935037680053
14	14.641499771559968	44.97457035701913	26.958936021729418	13.42499384969148
15	13.899697254201037	21.448761654207065	51.72560084748741	12.925940244104488
16	14.520250832174398	16.86590319163357	50.78698845750262	17.82685751868941
17	15.65592412778584	17.54795985480251	29.26567826606486	37.53043775134679
18	21.460058139242985	22.566862639762622	34.374199832309955	21.598879388684438
19	30.262028246233253	21.905892748660737	26.953162262711057	20.878916742394953
20	33.09920322125547	21.978441285891442	24.409695898622832	20.512659594230257
21	23.109595987488515	27.166791346390397	28.31401216003856	21.40960050608253
22	22.038438173082234	24.98656973445729	26.2442450684567	26.730747024003776
23	19.88281779522736	30.161112979912343	25.33575663856771	24.620312586292595
24	19.989506820566632	23.062401783338437	39.85048474472454	17.09760665137039
25	16.357059299015447	24.452120475757745	37.785487280157845	21.40533294506896
26	16.20769466354047	35.036675921416624	28.82386018465988	19.931769230383026
27	17.91722939897679	33.82167619755293	30.164376408922717	18.09671799454756
28	14.349799424632362	28.734492436375685	40.148209883671306	16.767498255320646
29	14.77881482299663	24.181004834895596	40.02294441627296	21.01723592583481
30	18.239053706000192	29.602062487134557	32.71612686203728	19.442756944827966
31	29.570432329033974	26.38231321889576	24.29597794926121	19.75127650280906
32	31.423055874925314	26.661210882782648	26.029611852774163	15.886121389517866
33	29.0251886513001	27.14595560732414	25.302871315463133	18.525984425912633
34	20.292001586528567	27.06662917907188	28.68754926522641	23.95381996917315
35	20.435341429984387	25.345797958599636	31.266160249426388	22.952700361989585
36	31.09821917189234	24.465174191799257	26.175462026237966	18.26114461007044
37	21.078990044031187	32.45153807919589	28.64889018310347	17.82058169366945
38	21.031042740878718	33.469978963434535	23.56095332292383	21.93802497276292
39	21.48566350532441	32.07950717201283	26.318801869693793	20.116027452968968
40	24.419737218654763	26.186256445272292	24.44810394774497	24.94590238832797
41	17.495996023637268	24.548768181065086	26.29269443761077	31.66254135768688
42	22.54677999969876	25.62670388649292	23.0533645953097	28.773151518498626
43	22.64920146402446	26.978014529790084	25.113090366859623	25.259693639325825
44	19.98423512754987	31.560119893361183	27.37841216606335	21.0772328130256
45	17.597664388960574	37.04092339979014	22.76894420540524	22.59246800584405
46	21.414872199099293	33.78326814843079	24.899210250179486	19.902649402290425
47	21.836607640440413	27.240595048625092	27.297077473804706	23.62571983712979
48	24.012310658359148	22.744091938326214	30.46410981187587	22.77948759143877
49	20.5061327362095	23.399539103410536	32.5082715373763	23.58605662300366
50	19.012486381459706	33.746617330314244	26.011788509717487	21.229107778508563
51	19.041857242553107	34.456538656571794	23.257956490960304	23.2436476099148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	313.5
2	474.0
3	2028.0
4	3582.0
5	2798.0
6	2014.0
7	2029.5
8	2045.0
9	2179.5
10	2314.0
11	2270.0
12	2226.0
13	2149.5
14	2073.0
15	1968.0
16	1863.0
17	1832.5
18	1802.0
19	1647.5
20	1493.0
21	1580.0
22	1667.0
23	1716.5
24	1766.0
25	2143.5
26	2891.5
27	3262.0
28	4031.5
29	4801.0
30	5531.5
31	6262.0
32	7015.5
33	7769.0
34	8367.0
35	8965.0
36	10316.5
37	11668.0
38	16705.0
39	21742.0
40	29393.0
41	37044.0
42	40297.5
43	43551.0
44	41055.5
45	38560.0
46	36094.0
47	33628.0
48	31433.0
49	29238.0
50	27326.5
51	25415.0
52	23834.0
53	22253.0
54	20470.0
55	18687.0
56	17129.5
57	15572.0
58	14360.5
59	13149.0
60	11904.5
61	10660.0
62	9049.0
63	7438.0
64	6026.5
65	4615.0
66	3743.5
67	2872.0
68	2449.0
69	2026.0
70	1662.0
71	1298.0
72	1087.5
73	877.0
74	664.5
75	362.0
76	272.0
77	203.5
78	135.0
79	112.5
80	90.0
81	64.0
82	38.0
83	25.5
84	13.0
85	10.5
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.93156024176844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.33858861870216	17.461946673615383
2	7.051691332885943	3.657227172101765
3	3.752268820923621	2.9190655491927098
4	2.683699611150122	2.7837007254939974
5	2.072546563892959	2.6872183037730233
6	1.6930676714339148	2.6342331789107516
7	1.473433505189228	2.674590079143815
8	1.2757758967299555	2.64662876168392
9	1.1320262469742397	2.6419686136807985
>10	11.349211552235692	55.94807094755427
>50	0.15872325056886927	2.6026211435087916
>100	0.015972151000640932	0.5889161628687588
>500	9.982594375400583E-4	0.21848142481812413
>1k	0.0019965188750801165	0.5353312636538872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1044	0.26207845283340947	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.2570577928174438	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.2118718526737525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007782023024746833	0.0
2	0.0	0.0	0.0	0.044432841141296434	0.0
3	0.0	0.0	0.0	0.05623139217881583	0.0
4	0.0	0.0	0.0	0.08459812126902203	0.0
5	0.0	0.0	0.0	0.143590876456619	0.0
6	0.0	0.0	0.0	0.2003243346370314	0.0
7	0.0	0.0	0.0	0.2429999447727398	0.0
8	0.0	0.0	0.0	0.47570753651274994	0.0
9	0.0	0.0	0.0	0.5495112387474458	0.0
10	0.0	0.0	0.0	0.7124316562655326	0.0
11	0.0	0.0	0.0	0.8705824467684522	0.0
12	0.0	0.0	0.0	0.9978561781731826	0.0
13	0.0	0.0	0.0	1.0573509993623762	0.0
14	0.0	0.0	0.0	1.077433639426239	0.0
15	0.0	0.0	0.0	1.1183520185563594	0.0
16	0.0	0.0	0.0	1.2021970408229865	0.0
17	0.0	0.0	0.0	1.3176722211901977	0.0
18	0.0	0.0	0.0	1.4662837576627823	0.0
19	0.0	0.0	0.0	1.534815766880714	0.0
20	0.0	0.0	0.0	1.6048539741034356	0.0
21	0.0	0.0	0.0	1.7070244054283377	0.0
22	0.0	0.0	0.0	1.8059314077428619	0.0
23	0.0	0.0	0.0	1.9342092711507854	0.0
24	0.0	0.0	0.0	2.015794996410228	0.0
25	0.0	0.0	0.0	2.0843270056281598	0.0
26	0.0	0.0	0.0	2.1588838068652505	0.0
27	0.0	0.0	0.0	2.2276668490839806	0.0
28	0.0	0.0	0.0	2.307244310337037	0.0
29	0.0	0.0	0.0	2.388579002595681	0.0
30	0.0	0.0	0.0	2.481963278892643	0.0
31	0.0	0.0	0.0	2.5926688322446867	0.0
32	0.0	0.0	0.0	2.683793811534464	0.0
33	0.0	0.0	0.0	2.775922922827435	0.0
34	0.0	0.0	0.0	2.868554100122002	0.0
35	0.0	0.0	0.0	3.0048650195554707	0.0
36	0.0	0.0	0.0	3.1168257379115056	0.0
37	0.0	0.0	0.0	3.2242678622531717	0.0
38	0.0	0.0	0.0	3.326438293578074	0.0
39	0.0	0.0	0.0	3.429612856906169	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCG	30	2.161918E-6	45.000004	41
GCGATGA	60	0.0	45.000004	9
TTGCATA	30	2.161918E-6	45.000004	1
TCCCCGC	30	2.161918E-6	45.000004	32
ACGGTCT	60	0.0	45.000004	30
AGCGTAA	50	2.1827873E-11	45.0	1
GGTACTA	35	1.2092642E-7	45.0	38
CGAAATG	20	7.027731E-4	45.0	45
TTTACGG	35	1.2092642E-7	45.0	2
TATGGTA	20	7.027731E-4	45.0	1
CCGGATA	35	1.2092642E-7	45.0	20
GCCGGAT	35	1.2092642E-7	45.0	19
TACGGGT	20	7.027731E-4	45.0	4
CGACAAG	20	7.027731E-4	45.0	2
ATACTCG	45	3.8380676E-10	45.0	44
TGATTCG	20	7.027731E-4	45.0	15
TTAAGCC	20	7.027731E-4	45.0	14
CGCTAAG	20	7.027731E-4	45.0	2
CAAGGCG	20	7.027731E-4	45.0	5
GCGATAT	20	7.027731E-4	45.0	9
>>END_MODULE