FastQCFastQC Report
Thu 26 May 2016
SRR1546409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546409_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences710004
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29270.4122511985847967No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.292251874637326No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.12506971791708216No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.12295705376307738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA302.5263435E-644.00000430
TAGTTAC302.5263435E-644.00000415
CGGATCC302.5263435E-644.00000444
AAGCACG302.5263435E-644.0000041
TACGAAC302.5263435E-644.00000438
ACGGATC302.5263435E-644.00000443
CGCAGGA302.5263435E-644.0000044
GTATACG302.5263435E-644.0000041
CGCAAAT302.5263435E-644.00000431
TTCGAAA302.5263435E-644.0000041
ACGGTCT302.5263435E-644.00000430
CAGCGAA254.4411165E-544.01
TCGTTGC207.8544463E-444.011
TCGTTAA207.8544463E-444.01
GTACCGT207.8544463E-444.016
ACACGCT254.4411165E-544.023
CAAGTCG254.4411165E-544.019
CGAGTGA207.8544463E-444.030
TCCGATA207.8544463E-444.013
TAGACGC207.8544463E-444.043
Produced by FastQC (version 0.11.5)