FastQCFastQC Report
Thu 26 May 2016
SRR1546408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546408_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences267959
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10830.40416630902488815No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.19779145317007454No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.17689273358984023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCT253.8827733E-545.0000045
AATCCCC253.8827733E-545.00000437
GATACCC253.8827733E-545.00000425
ACACGGG253.8827733E-545.0000043
ATTAGGC253.8827733E-545.0000044
ATAGGTC253.8827733E-545.0000045
TTAGCTA253.8827733E-545.0000041
CGACGGG253.8827733E-545.0000043
ACGTTGA253.8827733E-545.0000041
GCCGATT253.8827733E-545.0000049
CTAGTAG253.8827733E-545.0000042
GAGCGTA253.8827733E-545.00000436
TCATGTA253.8827733E-545.0000041
TCGTCCT253.8827733E-545.00000410
GTCGTCC253.8827733E-545.0000049
CGTACTA253.8827733E-545.00000439
GCGCTCC253.8827733E-545.00000429
CTTAGGC253.8827733E-545.0000044
CGTCAAG253.8827733E-545.0000042
GCGTACT253.8827733E-545.00000438