##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546408_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	267959
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.017301154281064	31.0	31.0	33.0	30.0	33.0
2	31.1749707977713	31.0	31.0	33.0	30.0	34.0
3	31.161819532092597	31.0	31.0	34.0	28.0	34.0
4	35.11024447769994	35.0	35.0	37.0	33.0	37.0
5	32.86112800838934	35.0	35.0	37.0	28.0	37.0
6	34.03192652607302	35.0	35.0	37.0	28.0	37.0
7	15.186584514795173	0.0	0.0	35.0	0.0	37.0
8	24.83800879985371	17.0	17.0	35.0	17.0	37.0
9	32.67294250239775	32.0	32.0	37.0	27.0	39.0
10	35.5376792718289	37.0	35.0	37.0	32.0	39.0
11	36.49242981202348	37.0	35.0	39.0	32.0	39.0
12	36.20090386962185	37.0	35.0	39.0	32.0	39.0
13	35.72789120723693	37.0	35.0	39.0	31.0	39.0
14	36.762728626394335	39.0	35.0	40.0	31.0	41.0
15	37.20036274206129	39.0	35.0	41.0	32.0	41.0
16	37.30791277770107	39.0	35.0	41.0	32.0	41.0
17	37.313297929907186	39.0	35.0	41.0	32.0	41.0
18	37.20191521837296	39.0	35.0	40.0	32.0	41.0
19	37.111128941367895	39.0	35.0	40.0	32.0	41.0
20	37.18873782929478	39.0	35.0	40.0	32.0	41.0
21	37.03791998029549	39.0	35.0	40.0	32.0	41.0
22	37.099705551968775	39.0	35.0	40.0	32.0	41.0
23	37.10070570497725	39.0	35.0	40.0	32.0	41.0
24	36.925652805093314	38.0	35.0	40.0	32.0	41.0
25	36.68056307121612	38.0	35.0	40.0	31.0	41.0
26	36.5550289409947	38.0	35.0	40.0	31.0	41.0
27	36.426651092144695	38.0	35.0	40.0	31.0	41.0
28	36.353360028959656	38.0	35.0	40.0	31.0	41.0
29	36.44343351035047	38.0	35.0	40.0	31.0	41.0
30	36.21227128030781	38.0	35.0	40.0	30.0	41.0
31	36.08864415824809	38.0	35.0	40.0	30.0	41.0
32	35.96627842319161	38.0	34.0	40.0	30.0	41.0
33	35.7626129370538	38.0	34.0	40.0	29.0	41.0
34	35.62861109348818	38.0	34.0	40.0	29.0	41.0
35	35.46684007628033	38.0	34.0	40.0	28.0	41.0
36	35.23781996499464	38.0	34.0	40.0	27.0	41.0
37	35.039259737497154	38.0	34.0	40.0	26.0	41.0
38	35.03746468676178	38.0	34.0	40.0	26.0	41.0
39	34.974316966401574	38.0	34.0	40.0	26.0	41.0
40	34.80234662765572	37.0	34.0	40.0	24.0	41.0
41	34.73997141353715	37.0	34.0	40.0	24.0	41.0
42	34.67565187211476	37.0	33.0	40.0	24.0	41.0
43	34.59434838911923	37.0	33.0	40.0	24.0	41.0
44	34.379169947641245	37.0	33.0	40.0	23.0	41.0
45	34.312782925746106	37.0	33.0	40.0	23.0	41.0
46	34.29751939662411	37.0	33.0	40.0	23.0	41.0
47	34.25889408454278	36.0	33.0	40.0	23.0	41.0
48	34.2442575170082	36.0	33.0	40.0	23.0	41.0
49	34.158117473195524	36.0	33.0	40.0	24.0	41.0
50	34.05422098156808	36.0	33.0	40.0	24.0	41.0
51	33.86941285793722	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	5.0
15	6.0
16	33.0
17	66.0
18	137.0
19	279.0
20	557.0
21	865.0
22	1315.0
23	1936.0
24	2825.0
25	3743.0
26	4568.0
27	4861.0
28	5340.0
29	6393.0
30	7826.0
31	10305.0
32	13917.0
33	21386.0
34	27343.0
35	27328.0
36	30223.0
37	46007.0
38	42968.0
39	7721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.826577946626166	24.6858661213096	29.971749409424575	15.515806522639657
2	29.791497953045052	24.83775502968738	32.24747069514366	13.123276322123905
3	30.072137901693917	22.659809896290103	34.47579667038614	12.79225553162984
4	26.265585406722668	27.424344769162445	31.09654835254647	15.213521471568411
5	21.554790098485217	37.16799958202561	28.44203777443564	12.835172545053533
6	21.896633440190477	39.500445963748184	28.091611030045645	10.511309566015695
7	37.47774846151837	57.512529901962615	4.163323493519531	0.8463981429994888
8	87.94554390783664	3.7251967651767623	6.702891113939073	1.6263682130475183
9	84.43530540119943	4.833575285771331	8.042275124179445	2.688844188849787
10	58.02492172309943	20.253471613194556	12.691493847939423	9.030112815766591
11	54.05080628006523	17.43774234117906	17.496333394287934	11.015117984467773
12	46.42426639896402	20.8143783190712	20.983434032818455	11.777921249146324
13	20.912900854235165	44.45754760989554	21.32826290589232	13.301288629976973
14	14.021548072652907	45.54801294227848	26.40665176388925	14.02378722117936
15	12.698211293518785	22.009710440776388	52.68194014755989	12.61013811814494
16	14.443254378468348	15.459454618057238	51.20186297157401	18.8954280319004
17	15.32585208931217	17.100004105105633	28.81075089845835	38.76339290712385
18	21.561507544064575	21.938804070772022	34.71949066834852	21.780197716814886
19	31.664545695423552	22.327296340111733	25.10570647001967	20.902451494445046
20	34.05931504446576	21.357745028157293	24.880298851689997	19.70264107568695
21	23.396116570072287	27.890460854085887	27.08884568161547	21.624576894226355
22	23.614060359980446	24.62242357972675	24.41642191529301	27.347094144999794
23	20.72742471796058	30.75246586231476	24.566444866565405	23.95366455315925
24	20.46133923473367	22.50866737075448	40.038961184360296	16.991032210151552
25	16.699196518870423	24.584358054777038	37.33892125287824	21.3775241734743
26	16.40512167906284	35.67933900335499	27.59937154564691	20.31616777193526
27	18.210621774226652	35.163588459428496	29.175732108270296	17.450057658074556
28	14.838090901966345	28.873447057199048	40.422228773804946	15.866233267029658
29	15.127314253299945	24.001806246478004	39.528062128907784	21.34281737131427
30	18.468870237610975	31.035344959490068	31.311879802507097	19.183905000391853
31	29.45711843976131	26.732447874488262	24.550770826880232	19.2596628588702
32	32.08214689560717	26.11295011550274	25.713635294951843	16.091267693938253
33	29.66200052993182	27.309775002892234	24.156680686224384	18.871543780951562
34	19.62203172873462	27.112356741143234	28.016226362988366	25.249385167133774
35	20.455741363417538	24.767221851104086	31.265604066293722	23.51143271918465
36	31.687310372109167	24.014867946215652	26.078243313342714	18.219578368332467
37	20.910288514287632	33.03938289066611	28.969357252415485	17.080971342630775
38	19.83363126448449	33.442056434006695	23.51926973902724	23.205042562481573
39	21.02299232345247	32.32919961635922	27.15975205161984	19.488056008568474
40	24.351486608025855	26.029355237181807	24.773939296683448	24.84521885810889
41	17.34108576312048	24.210793442280348	26.724610854645675	31.723509939953498
42	22.552703958441402	24.571669546460466	23.935378173526548	28.94024832157158
43	22.69339712418691	26.6798278841166	24.894480125690873	25.732294866005624
44	19.82355509611545	30.75209267089368	27.81619576129184	21.60815647169903
45	17.42094872723066	38.63538824969492	21.839161961344832	22.104501061729593
46	21.82684664444934	34.96654338910057	23.839467978310115	19.367141988139977
47	22.539642258703758	27.483682205113467	26.05137353102527	23.925302005157505
48	23.855515209416367	22.72996988345232	31.099533883915075	22.314981023216237
49	20.50574901384167	23.832004149888604	32.460562996577835	23.201683839691896
50	18.71890848973164	34.38996264353875	25.931579084859997	20.95954978186961
51	18.123668173116037	35.798013875257034	22.600472460339084	23.477845491287848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	195.5
2	303.0
3	1174.5
4	2046.0
5	1647.5
6	1249.0
7	1265.5
8	1282.0
9	1296.5
10	1311.0
11	1356.5
12	1402.0
13	1314.0
14	1226.0
15	1172.5
16	1119.0
17	1057.5
18	996.0
19	928.0
20	860.0
21	918.5
22	977.0
23	1046.5
24	1116.0
25	1305.5
26	2025.5
27	2556.0
28	3107.5
29	3659.0
30	4042.0
31	4425.0
32	4978.5
33	5532.0
34	5809.5
35	6087.0
36	6973.5
37	7860.0
38	11532.5
39	15205.0
40	19795.5
41	24386.0
42	27146.5
43	29907.0
44	28235.0
45	26563.0
46	24722.0
47	22881.0
48	21041.5
49	19202.0
50	18196.5
51	17191.0
52	15754.5
53	14318.0
54	13010.5
55	11703.0
56	10772.5
57	9842.0
58	9514.0
59	9186.0
60	8236.0
61	7286.0
62	6185.0
63	5084.0
64	4160.5
65	3237.0
66	2723.0
67	2209.0
68	1890.5
69	1572.0
70	1263.5
71	955.0
72	810.5
73	666.0
74	563.0
75	366.0
76	272.0
77	215.5
78	159.0
79	110.0
80	61.0
81	38.0
82	15.0
83	10.5
84	6.0
85	3.5
86	1.0
87	2.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	267959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.458085005541893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69639110365087	18.980515675905643
2	6.9017519932857745	3.9282128982418953
3	3.9983738984473356	3.413581928578626
4	2.8587914393621485	3.254229191779339
5	2.271296684851028	3.2318377065148027
6	1.938208140998741	3.30946152209853
7	1.6523289970625261	3.2915483338869005
8	1.4530004196391104	3.307968756414228
9	1.3782522031053295	3.530017651954217
>10	10.774234158623583	51.44331782100993
>50	0.0642572387746538	1.1554006396500958
>100	0.01049097775912715	0.5519501117708306
>500	0.0013113722198908938	0.19779145317007454
>1k	0.0013113722198908938	0.40416630902488815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1083	0.40416630902488815	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.19779145317007454	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.17689273358984023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010822551211192757	0.0
2	0.0	0.0	0.0	0.03657275926541001	0.0
3	0.0	0.0	0.0	0.04814169331875399	0.0
4	0.0	0.0	0.0	0.06829403005683705	0.0
5	0.0	0.0	0.0	0.10971827779623002	0.0
6	0.0	0.0	0.0	0.1485301855880937	0.0
7	0.0	0.0	0.0	0.16868252232617675	0.0
8	0.0	0.0	0.0	0.32206419638825345	0.0
9	0.0	0.0	0.0	0.3799088666549733	0.0
10	0.0	0.0	0.0	0.5086599069260596	0.0
11	0.0	0.0	0.0	0.6351717986706922	0.0
12	0.0	0.0	0.0	0.7538466705727369	0.0
13	0.0	0.0	0.0	0.8019883638914909	0.0
14	0.0	0.0	0.0	0.8221407006295739	0.0
15	0.0	0.0	0.0	0.8516228228945473	0.0
16	0.0	0.0	0.0	0.9146921730563258	0.0
17	0.0	0.0	0.0	0.9979138599561873	0.0
18	0.0	0.0	0.0	1.1124836262264004	0.0
19	0.0	0.0	0.0	1.1665963822823642	0.0
20	0.0	0.0	0.0	1.2281729667598402	0.0
21	0.0	0.0	0.0	1.3016916767117357	0.0
22	0.0	0.0	0.0	1.385286555032673	0.0
23	0.0	0.0	0.0	1.4729865389854417	0.0
24	0.0	0.0	0.0	1.5506103545691692	0.0
25	0.0	0.0	0.0	1.6140528961520233	0.0
26	0.0	0.0	0.0	1.6782418205770286	0.0
27	0.0	0.0	0.0	1.7342205337383705	0.0
28	0.0	0.0	0.0	1.795423926794771	0.0
29	0.0	0.0	0.0	1.8618519997462297	0.0
30	0.0	0.0	0.0	1.940595389593184	0.0
31	0.0	0.0	0.0	2.015606865229382	0.0
32	0.0	0.0	0.0	2.0853936609705217	0.0
33	0.0	0.0	0.0	2.152194925343056	0.0
34	0.0	0.0	0.0	2.229818740926784	0.0
35	0.0	0.0	0.0	2.3358051045122576	0.0
36	0.0	0.0	0.0	2.437313171044824	0.0
37	0.0	0.0	0.0	2.519042092260383	0.0
38	0.0	0.0	0.0	2.6082348418974544	0.0
39	0.0	0.0	0.0	2.684739083217955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCT	25	3.8827733E-5	45.000004	5
AATCCCC	25	3.8827733E-5	45.000004	37
GATACCC	25	3.8827733E-5	45.000004	25
ACACGGG	25	3.8827733E-5	45.000004	3
ATTAGGC	25	3.8827733E-5	45.000004	4
ATAGGTC	25	3.8827733E-5	45.000004	5
TTAGCTA	25	3.8827733E-5	45.000004	1
CGACGGG	25	3.8827733E-5	45.000004	3
ACGTTGA	25	3.8827733E-5	45.000004	1
GCCGATT	25	3.8827733E-5	45.000004	9
CTAGTAG	25	3.8827733E-5	45.000004	2
GAGCGTA	25	3.8827733E-5	45.000004	36
TCATGTA	25	3.8827733E-5	45.000004	1
TCGTCCT	25	3.8827733E-5	45.000004	10
GTCGTCC	25	3.8827733E-5	45.000004	9
CGTACTA	25	3.8827733E-5	45.000004	39
GCGCTCC	25	3.8827733E-5	45.000004	29
CTTAGGC	25	3.8827733E-5	45.000004	4
CGTCAAG	25	3.8827733E-5	45.000004	2
GCGTACT	25	3.8827733E-5	45.000004	38
>>END_MODULE