FastQCFastQC Report
Thu 26 May 2016
SRR1546407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546407_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1720526
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.3591924795091734No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA56570.32879479879990187No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.1577424578297567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.14344450476191584No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20450.11885900009648213No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17830.1036310988616272No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17700.1028755159759283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA408.3164196E-944.036
CGTATCT351.4466423E-744.036
GGTCGTC302.5284535E-644.08
GGTCGTA207.857575E-444.08
ACGTTAT302.5284535E-644.033
GACGTAG207.857575E-444.02
CGGCACT207.857575E-444.042
TTCGTTA351.4466423E-744.01
ATCGTCC207.857575E-444.025
GTAATCG207.857575E-444.022
ATTCACG254.443765E-544.01
GTCACGA302.5284535E-644.042
CGTAAGA254.443765E-544.03
CGTCGGT302.5284535E-644.019
CCGAATC302.5284535E-644.028
CACGTTA408.3164196E-944.01
GTGCGTA207.857575E-444.01
ATCGCGA302.5284535E-644.01
AGTACGT408.3164196E-944.015
GTACGTT207.857575E-444.036
Produced by FastQC (version 0.11.5)